GEODESIC LENGTH MORPHOMETRY: A simple method for quantifying severe degeneration laterally in rodent models of retinal photic injury.
4 affiliations
- 1Institute of Photonics Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Chuo-ku, Hamamatsu, 431-3192, Japan.
- 2Department of Ophthalmology, Seirei Hamamatsu General Hospital, 2-12-12 Sumiyoshi, Chuo-ku, Hamamatsu, 430-8558, Japan.
- 3Department of Ophthalmology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Chuo-ku, Hamamatsu, 431-3192, Japan.
- 4Organization for Innovation and Social Collaboration, Shizuoka University, 3-5-1 Johoku, Chuo-ku, Hamamatsu, 432-8561, Japan.
Abstract
Quantification of degeneration extent in curvilinear microscopic specimens, such as the retina, is challenging due to inherent geometric complexity. In the rodent retinal photic injury model, degeneration arc lengths are a critical, yet difficult-to-quantify parameter for assessing severity. We developed a novel, precise platform, termed "Geodesic Length Morphometry," that quantifies the actual arc lengths of curvilinear structures using a precisely determined digital scale factor. In this methodology, defining a curved coordinate axis along the retinal contour enables precise numerical localization of any position and the measurement of dorsoventral arc lengths between any two points. This robust platform resulted in two analytical innovations. One is a new visualization method, termed "Geodesic Equidistant-Projection Plot," which converts arc length data measured from curved anatomical imagery into a cumulative line chart, allowing for the direct visual comparison of actual degeneration arc length and location. The second is the definition of the "Geodesic Index" (0 ≤ x ≤ 1), which normalizes the degeneration arc length against the entire retinal arc length to enable an objective, size-independent comparison of extent of degeneration. The Geodesic Length Morphometry platform offers a robust standard for the quantitative analysis of curvilinear tissue arc length and serves as a versatile tool applicable to diverse biological and morphometric fields.
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BibTeX
@article{ohishi2026geodesic,
title = {{GEODESIC LENGTH MORPHOMETRY: A simple method for quantifying severe degeneration laterally in rodent models of retinal photic injury.}},
author = {Kentaro Ohishi and Masafumi Ohtsubo and Akira Obana and Yoshihiro Hotta and Yu Nagashima and Shinsei Minoshima},
journal = {MethodsX},
year = {2026},
month = sep,
doi = {10.1016/j.mex.2026.104183},
url = {https://europepmc.org/article/MED/42927176},
note = {CC BY},
}
RIS
TY - JOUR
TI - GEODESIC LENGTH MORPHOMETRY: A simple method for quantifying severe degeneration laterally in rodent models of retinal photic injury.
AU - Kentaro Ohishi
AU - Masafumi Ohtsubo
AU - Akira Obana
AU - Yoshihiro Hotta
AU - Yu Nagashima
AU - Shinsei Minoshima
PY - 2026
DA - 2026/09/29
JO - MethodsX
DO - 10.1016/j.mex.2026.104183
UR - https://europepmc.org/article/MED/42927176
AB - Quantification of degeneration extent in curvilinear microscopic specimens, such as the retina, is challenging due to inherent geometric complexity. In the rodent retinal photic injury model, degeneration arc lengths are a critical, yet difficult-to-quantify parameter for assessing severity. We developed a novel, precise platform, termed "Geodesic Length Morphometry," that quantifies the actual arc lengths of curvilinear structures using a precisely determined digital scale factor. In this methodology, defining a curved coordinate axis along the retinal contour enables precise numerical localization of any position and the measurement of dorsoventral arc lengths between any two points. This robust platform resulted in two analytical innovations. One is a new visualization method, termed "Geodesic Equidistant-Projection Plot," which converts arc length data measured from curved anatomical imagery into a cumulative line chart, allowing for the direct visual comparison of actual degeneration arc length and location. The second is the definition of the "Geodesic Index" (0 ≤ x ≤ 1), which normalizes the degeneration arc length against the entire retinal arc length to enable an objective, size-independent comparison of extent of degeneration. The Geodesic Length Morphometry platform offers a robust standard for the quantitative analysis of curvilinear tissue arc length and serves as a versatile tool applicable to diverse biological and morphometric fields.
SN - 2215-0161
ER -