Peer-reviewedCC BYSTAR Protocols
Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing
Abstract
Single-nucleus RNA sequencing (snRNA-seq) enables transcriptomic profiling of tissues that are difficult to dissociate. Here, we present an integrated protocol combining tissue-specific nuclei extraction and selective enrichment to isolate high-quality nuclei from fresh mouse liver and visceral adipose tissue (VAT). We describe steps for single-nucleus extraction, magnetic enrichment, BD Rhapsody capture, cDNA synthesis, whole-transcriptome amplification, index PCR, library quality control, and shallow sequencing-based assessment of cell-type recovery, while separating whole-liver and non-parenchymal cell workflows to reduce dissociation bias.
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BibTeX
@article{li2026protocol,
title = {{Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing}},
author = {Lei Li and Jonathan Pol and Christophe Desterke and Wanchao Hu and Nicolas Trainel and Morgane Benardeau and Manuela Lizarralde-Guerrero and Maria Chiara Maiuri and Guido Kroemer and Gabriel Perlemuter and Anne-Marie Cassard and Cosmin Sebastian Voican},
journal = {STAR Protocols},
year = {2026},
month = jul,
doi = {10.1016/j.xpro.2026.104699},
url = {https://hal.science/hal-05793481v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing
AU - Lei Li
AU - Jonathan Pol
AU - Christophe Desterke
AU - Wanchao Hu
AU - Nicolas Trainel
AU - Morgane Benardeau
AU - Manuela Lizarralde-Guerrero
AU - Maria Chiara Maiuri
AU - Guido Kroemer
AU - Gabriel Perlemuter
AU - Anne-Marie Cassard
AU - Cosmin Sebastian Voican
PY - 2026
DA - 2026/07/08
JO - STAR Protocols
DO - 10.1016/j.xpro.2026.104699
UR - https://hal.science/hal-05793481v1
AB - Single-nucleus RNA sequencing (snRNA-seq) enables transcriptomic profiling of tissues that are difficult to dissociate. Here, we present an integrated protocol combining tissue-specific nuclei extraction and selective enrichment to isolate high-quality nuclei from fresh mouse liver and visceral adipose tissue (VAT). We describe steps for single-nucleus extraction, magnetic enrichment, BD Rhapsody capture, cDNA synthesis, whole-transcriptome amplification, index PCR, library quality control, and shallow sequencing-based assessment of cell-type recovery, while separating whole-liver and non-parenchymal cell workflows to reduce dissociation bias.
SN - 2666-1667
ER -