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PreprintCC BYbioRxiv

Electrical Stunning does not affect Brain Tissue Integrity and Stress Response in Larval Zebrafish

  • D.-S. Burkhardt
  • K. Schultz
  • M. Glaser
  • S. Krais
  • D. Maile
  • H.-R. Koehler
  • R. Triebskorn
  • A. B. Arrenberg1
  1. 1Werner Reichardt Centre for Integrative Neuroscience, University of Tuebingen
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Abstract

Electrical stunning is a humane euthanasia method for larval zebrafish, and a suitable euthanasia protocol has recently been established. Here, we assess the associated stress response and tissue integrity. When compared to MS-222 overdose and hypothermia, electrical stunning caused no significant Hsp70 increase. Brain tissue was preserved and muscle tissue affected only slightly. Our results support the suitability of electrical stunning for humane euthanasia and artifact-free post-mortem analyses.

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Cite

BibTeXRIS
BibTeX
@misc{burkhardt2026electrical,
  title = {{Electrical Stunning does not affect Brain Tissue Integrity and Stress Response in Larval Zebrafish}},
  author = {D.-S. Burkhardt and K. Schultz and M. Glaser and S. Krais and D. Maile and H.-R. Koehler and R. Triebskorn and A. B. Arrenberg},
  howpublished = {bioRxiv},
  year = {2026},
  month = sep,
  doi = {10.64898/2026.09.04.749313},
  url = {https://www.biorxiv.org/content/10.64898/2026.09.04.749313v1},
  note = {CC BY},
}
RIS
TY  - UNPB
TI  - Electrical Stunning does not affect Brain Tissue Integrity and Stress Response in Larval Zebrafish
AU  - D.-S. Burkhardt
AU  - K. Schultz
AU  - M. Glaser
AU  - S. Krais
AU  - D. Maile
AU  - H.-R. Koehler
AU  - R. Triebskorn
AU  - A. B. Arrenberg
PY  - 2026
DA  - 2026/09/04
PB  - bioRxiv
DO  - 10.64898/2026.09.04.749313
UR  - https://www.biorxiv.org/content/10.64898/2026.09.04.749313v1
AB  - Electrical stunning is a humane euthanasia method for larval zebrafish, and a suitable euthanasia protocol has recently been established. Here, we assess the associated stress response and tissue integrity. When compared to MS-222 overdose and hypothermia, electrical stunning caused no significant Hsp70 increase. Brain tissue was preserved and muscle tissue affected only slightly. Our results support the suitability of electrical stunning for humane euthanasia and artifact-free post-mortem analyses.
ER  -