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Peer-reviewedCC BY-NC-ND 4.0Scientific Data

A near telomere-to-telomere genome assembly of Rhodiola macrocarpa (Crassulaceae)

  • Ning Miao1,2
  • Xinrui Song1,2
  • Hongqiang Lin3
  • Rui Li4
  • Guanghui Yang1,2
  • Yujie Huang1,2
  • Qi Zhao4
  • Mingcheng Wang4,5
5 affiliations
  1. 1Southwest Bio-resources R&D Key Laboratory of Sichuan Province, Chengdu Botanical Garden-Sichuan University Joint Laboratory for Ex Situ Conservation and Resource Utilization of Mountain Plants, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064 Sichuan China
  2. 2Chengdu Botanical Garden, Chengdu, China
  3. 3Sichuan Wolong National Natural Reserve Administration Bureau, Wenchuan, 623006 China
  4. 4Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, Chengdu University, Chengdu, 610106 China
  5. 5Institute for Advanced Study, Chengdu University, No. 2025 Chengluo Road, Chengdu, 610106 China
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Abstract

Rhodiola macrocarpa, a medicinal species of the family Crassulaceae, is distributed in regions of southwestern China and northern Myanmar. Here, we present a near telomere-to-telomere (T2T) chromosome-level genome assembly generated using Oxford Nanopore Technologies long reads in combination with Hi-C scaffolding. The final assembly spans 727.48 Mb and exhibits a contig N50 of 71.78 Mb. Notably, 99.80% of the assembled sequence was anchored to ten pseudo-chromosomes, six of which are gap-free, while the remaining four contain a single gap each. Assembly evaluation showed a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness of 97.71% and a Long Terminal Repeat Assembly Index (LAI) of 15.81, both indicating reference-level quality. Telomeric repeat motifs were identified at both ends of seven pseudo-chromosomes, and centromeric regions were identified across all ten chromosomes. Genome annotation predicted 37,627 protein-coding genes, of which 93.69% were functionally annotated, along with 1,753 transcription factors and 6,580 non-coding RNAs. This near T2T genome assembly provides a high-quality genomic resource for comparative studies and for investigating high-altitude adaptation, specialized metabolite biosynthesis, and the evolutionary history of the genus Rhodiola.

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Cite

BibTeXRIS
BibTeX
@article{miao2026near,
  title = {{A near telomere-to-telomere genome assembly of Rhodiola macrocarpa (Crassulaceae)}},
  author = {Ning Miao and Xinrui Song and Hongqiang Lin and Rui Li and Guanghui Yang and Yujie Huang and Qi Zhao and Mingcheng Wang},
  journal = {Scientific Data},
  year = {2026},
  month = jul,
  doi = {10.1038/s41597-026-07834-8},
  url = {https://doi.org/10.1038/s41597-026-07834-8},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - A near telomere-to-telomere genome assembly of Rhodiola macrocarpa (Crassulaceae)
AU  - Ning Miao
AU  - Xinrui Song
AU  - Hongqiang Lin
AU  - Rui Li
AU  - Guanghui Yang
AU  - Yujie Huang
AU  - Qi Zhao
AU  - Mingcheng Wang
PY  - 2026
DA  - 2026/07/06
JO  - Scientific Data
DO  - 10.1038/s41597-026-07834-8
UR  - https://doi.org/10.1038/s41597-026-07834-8
AB  - Rhodiola macrocarpa, a medicinal species of the family Crassulaceae, is distributed in regions of southwestern China and northern Myanmar. Here, we present a near telomere-to-telomere (T2T) chromosome-level genome assembly generated using Oxford Nanopore Technologies long reads in combination with Hi-C scaffolding. The final assembly spans 727.48 Mb and exhibits a contig N50 of 71.78 Mb. Notably, 99.80% of the assembled sequence was anchored to ten pseudo-chromosomes, six of which are gap-free, while the remaining four contain a single gap each. Assembly evaluation showed a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness of 97.71% and a Long Terminal Repeat Assembly Index (LAI) of 15.81, both indicating reference-level quality. Telomeric repeat motifs were identified at both ends of seven pseudo-chromosomes, and centromeric regions were identified across all ten chromosomes. Genome annotation predicted 37,627 protein-coding genes, of which 93.69% were functionally annotated, along with 1,753 transcription factors and 6,580 non-coding RNAs. This near T2T genome assembly provides a high-quality genomic resource for comparative studies and for investigating high-altitude adaptation, specialized metabolite biosynthesis, and the evolutionary history of the genus Rhodiola.
SN  - 2052-4463
ER  -