Neuronal primary cilia are not required for hippocampal circuit function or behavior in adult mice
- 1St. Jude Children's Research Hospital
Abstract
Primary cilia, which are present in most brain cells, are essential for brain development and function. During early brain development, dysfunction of the primary cilia can lead to a broad spectrum of disorders, collectively termed ciliopathies, that include brain malformations and intellectual disability. Although the role of primary cilia in brain development is well-established, cilia-mediated signaling in mature neurons and the contribution of cilia to neuronal circuit function remain controversial. Using mouse genetic and behavioral studies, single-cell electrophysiology, and 2-photon imaging, we show that deletion of primary cilia from adult hippocampal neurons is not required for hippocampal circuit function or behavior. Chronic genetic deletion or acute laser ablation of primary cilia from mature pyramidal neurons in the CA1 or CA3 regions of the hippocampus did not affect neuronal excitability, basal synaptic transmission, or long-term synaptic plasticity at excitatory CA3-CA1 hippocampal synapses. Moreover, the loss of primary cilia did not affect hippocampal-dependent learning and memory or anxiety-like behaviors. These results challenge the prevailing view of cilia function in mature hippocampal neurons and suggest that neuronal cilia in the adult hippocampus do not serve as major signaling hubs for pathways essential for neuronal function or behavior.
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BibTeX
@misc{eom2026neuronal,
title = {{Neuronal primary cilia are not required for hippocampal circuit function or behavior in adult mice}},
author = {T.-Y. Eom and I. T. Bayazitov and B. J. Teubner and D. Eddins and S. S. Zakharenko},
howpublished = {bioRxiv},
year = {2026},
month = aug,
doi = {10.64898/2026.08.24.746770},
url = {https://www.biorxiv.org/content/10.64898/2026.08.24.746770v2},
note = {CC BY},
}
RIS
TY - UNPB
TI - Neuronal primary cilia are not required for hippocampal circuit function or behavior in adult mice
AU - T.-Y. Eom
AU - I. T. Bayazitov
AU - B. J. Teubner
AU - D. Eddins
AU - S. S. Zakharenko
PY - 2026
DA - 2026/08/24
PB - bioRxiv
DO - 10.64898/2026.08.24.746770
UR - https://www.biorxiv.org/content/10.64898/2026.08.24.746770v2
AB - Primary cilia, which are present in most brain cells, are essential for brain development and function. During early brain development, dysfunction of the primary cilia can lead to a broad spectrum of disorders, collectively termed ciliopathies, that include brain malformations and intellectual disability. Although the role of primary cilia in brain development is well-established, cilia-mediated signaling in mature neurons and the contribution of cilia to neuronal circuit function remain controversial. Using mouse genetic and behavioral studies, single-cell electrophysiology, and 2-photon imaging, we show that deletion of primary cilia from adult hippocampal neurons is not required for hippocampal circuit function or behavior. Chronic genetic deletion or acute laser ablation of primary cilia from mature pyramidal neurons in the CA1 or CA3 regions of the hippocampus did not affect neuronal excitability, basal synaptic transmission, or long-term synaptic plasticity at excitatory CA3-CA1 hippocampal synapses. Moreover, the loss of primary cilia did not affect hippocampal-dependent learning and memory or anxiety-like behaviors. These results challenge the prevailing view of cilia function in mature hippocampal neurons and suggest that neuronal cilia in the adult hippocampus do not serve as major signaling hubs for pathways essential for neuronal function or behavior.
ER -