Profiling of Bioactive Metabolites and In Vitro Multifunctional Properties of Postbiotic Cell-Free Supernatant Derived from Probiotic Lactic Acid Bacteria Isolated from Fermented Olives
4 affiliations
- 1Research Unit of Microbiology, Biomolecules, and Biotechnology, Laboratory of Chemistry-Physics and Biotechnology of Molecules and Materials, Faculty of Sciences and Techniques—Mohammedia, Hassan II University of Casablanca, Casablanca 28806, Morocco
- 2Laboratory of Bioresources, Biotechnology, and Sustainable Development (L2BD), Faculty of Sciences, Mohammed Premier University, Oujda 60000, Morocco
- 3Laboratory of Natural Resources and Environment, Faculty of Applied Sciences of Taza, Sidi Mohamed Ben Abdellah University, B. P. 1223, Taza 35000, Morocco
- 4Department of Food Science and Agricultural Chemistry, Macdonald Campus, McGill University, 21111 Lakeshore, Sainte-Anne-de-Bellevue, QC H9X 3V9, Canada
Abstract
This work aims to characterize the bioactive metabolite profile and in vitro multifunctional properties of postbiotic cell-free supernatants (Postbiotic-CFSs) derived from three lactic acid bacteria (Levilactobacillus brevis S27, Lactiplantibacillus pentosus S42, and Lactiplantibacillus plantarum S62) isolated from fermented green olives. The postbiotic-CFSs were analyzed for their bioactive metabolites, emulsification index E24%, and antimicrobial, antioxidant, anti-inflammatory, and antidiabetic activities. The bioactive metabolites identified in all postbiotics included organic acids (lactate, citrate, and oxalate), SCFAs (acetate), hydrogen peroxide, diacetyl, exopolysaccharides (EPSs), proteins, biosurfactants, polyphenols, and flavonoids. The postbiotic-CFSs exhibited emulsification index (E24%) values ranging from 21.5% to 38.2%. Furthermore, they demonstrated antimicrobial properties with inhibition diameters ranging from 14.10 to 16.9 mm against pathogenic bacteria and from 15.8 to 23.50 mm against yeast, while fungal mycelial growth was inhibited by 35.77 to 45.09%. All postbiotics demonstrated antioxidant activities of DPPH from 65.91 to 68.55% and FRAP values from 1707.31 to 1765 µg AAE/mL, as well as anti-inflammatory activity (11.83–12.88%) and antidiabetic activity (34.38–93.75%). These findings highlight the significant potential of postbiotic-CFSs as natural bio-preservative agents in food products and as a source of bioactive metabolites with multifunctional health benefits for consumers.
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BibTeX
@article{abouloifa2026profiling,
title = {{Profiling of Bioactive Metabolites and In Vitro Multifunctional Properties of Postbiotic Cell-Free Supernatant Derived from Probiotic Lactic Acid Bacteria Isolated from Fermented Olives}},
author = {Houssam Abouloifa and Imane Maiouet and Asma Bouqafa and Nada Mouhtadi and Moussa Bouzzit and Nour Eddine Bentouhami and Nabil Ghabbour and Fouad Mellouki and Salwa Karboune},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27209138},
url = {https://www.mdpi.com/1422-0067/27/20/9138},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Profiling of Bioactive Metabolites and In Vitro Multifunctional Properties of Postbiotic Cell-Free Supernatant Derived from Probiotic Lactic Acid Bacteria Isolated from Fermented Olives
AU - Houssam Abouloifa
AU - Imane Maiouet
AU - Asma Bouqafa
AU - Nada Mouhtadi
AU - Moussa Bouzzit
AU - Nour Eddine Bentouhami
AU - Nabil Ghabbour
AU - Fouad Mellouki
AU - Salwa Karboune
PY - 2026
DA - 2026/10/01
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27209138
UR - https://www.mdpi.com/1422-0067/27/20/9138
AB - This work aims to characterize the bioactive metabolite profile and in vitro multifunctional properties of postbiotic cell-free supernatants (Postbiotic-CFSs) derived from three lactic acid bacteria (Levilactobacillus brevis S27, Lactiplantibacillus pentosus S42, and Lactiplantibacillus plantarum S62) isolated from fermented green olives. The postbiotic-CFSs were analyzed for their bioactive metabolites, emulsification index E24%, and antimicrobial, antioxidant, anti-inflammatory, and antidiabetic activities. The bioactive metabolites identified in all postbiotics included organic acids (lactate, citrate, and oxalate), SCFAs (acetate), hydrogen peroxide, diacetyl, exopolysaccharides (EPSs), proteins, biosurfactants, polyphenols, and flavonoids. The postbiotic-CFSs exhibited emulsification index (E24%) values ranging from 21.5% to 38.2%. Furthermore, they demonstrated antimicrobial properties with inhibition diameters ranging from 14.10 to 16.9 mm against pathogenic bacteria and from 15.8 to 23.50 mm against yeast, while fungal mycelial growth was inhibited by 35.77 to 45.09%. All postbiotics demonstrated antioxidant activities of DPPH from 65.91 to 68.55% and FRAP values from 1707.31 to 1765 µg AAE/mL, as well as anti-inflammatory activity (11.83–12.88%) and antidiabetic activity (34.38–93.75%). These findings highlight the significant potential of postbiotic-CFSs as natural bio-preservative agents in food products and as a source of bioactive metabolites with multifunctional health benefits for consumers.
SN - 1422-0067
ER -