Skip to content
DraconiticResearch
Search Sources API
203,234 studies31 sourcesUpdated 1 h ago
Peer-reviewedCC BYVeterinary sciences

Mechanistic Investigation of Cryptotanshinone and Curcumin Combination in Attenuating BVDV-1-Induced Injury in Bovine Ovarian Granulosa Cells.

  • Xiaoliang Chen1
  • Kang Zhang2
  • Haipeng Feng2
  • Lei Wang2
  • Jingyan Zhang2
  • Xiaorong Lu2
  • Guowei Xu2
  • Siqi Liu2,3
  • Xiaowei Feng2
  • Junyan Wang2,3
  • Jianxi Li1,2
  1. 1Hebei Key Laboratory of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071000, China.
  2. 2Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
  3. 3College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Read full textPDFDOICite

Abstract

Bovine viral diarrhea virus (BVDV) is a widespread pathogen causing significant economic losses in cattle. There is an urgent need for drugs to treat BVDV-induced reproductive dysfunction. This study investigated the mechanisms and targets of the cryptotanshinone-curcumin (CRY-CUR) combination against BVDV-1. Overlapping targets of CRY, CUR, and BVDV were screened using TCMSP, ChEMBL, and GeneCards databases. A Venn diagram was generated, followed by GO and KEGG enrichment analyses via DAVID. A PPI network was constructed using Cytoscape, and molecular docking was performed with AutoDock. Anti-BVDV-1 activity was verified in bovine ovarian granulosa cells (BOGCs) using RT-qPCR and immunofluorescence. A total of 108 overlapping targets were identified, with four core targets (STAT3, TNF, BCL-2, and EGFR) selected. Molecular docking showed strong binding affinities. Enrichment analysis indicated involvement in calcium ion binding, apoptosis, and TNF/NF-κB pathways. In vitro, the CRY-CUR combination suppressed BVDV-1 replication and reversed abnormal mRNA expression of the core targets. The CRY-CUR combination may inhibit BVDV-1 replication, potentially through modulation of BVDV-1-related targets such as STAT3, TNF, BCL-2, and EGFR and their associated signaling pathways.

Related

  1. Ultrasound-activated phase-change nanodroplets for root canal biofilm eradication: mechanistic Investigation and experimental validation
    Xiaoyu Qian, Jie Dang, Ziqi Shi +2
    Frontiers in Bioengineering and BiotechnologyPeer-reviewedCC BYFull textSystems & synthetic biologyDOAJ
  2. Mechanistic Investigation of Reversible Hibernation-Driven Immune Suppression in Thirteen-Lined Ground Squirrels
    M. Zhao, J. Chen, R. Burrett +7
    bioRxivPreprintCC BY-NC-NDFull textImmunologybioRxiv
  3. Identification and mechanistic investigation of novel biomarkers for diabetic retinopathy through hypoxia- and cuproptosis-associated gene analysis
    Rong Feng, Xia Wang, Xiaodong Li +4
    PLOS OnePeer-reviewedCC BY 4.0Full textCell biologyImmunologyPLOS
  4. The CLEC7A Y238∗ variant and susceptibility to pneumonia: a prospective population-based cohort study and mechanistic investigation.
    Oskar Thofte, Valdemar Öhnström, Linda Yamba Yamba +13
    EBioMedicinePeer-reviewedCC BY-NCFull textImmunologyEurope PMCCrossref
  5. Mechanistic investigation of clarithromycin in ulcerative colitis involving Kv1.3 downregulation and modulation of the Th17/Treg balance
    Jiaying Wu, Wen Zhang, Xuwen Mao
    Frontiers in ImmunologyPeer-reviewedCC BY 4.0Full textGenetics & genomicsCrossrefEurope PMCPMC

Cite

BibTeXRIS
BibTeX
@article{chen2026mechanistic,
  title = {{Mechanistic Investigation of Cryptotanshinone and Curcumin Combination in Attenuating BVDV-1-Induced Injury in Bovine Ovarian Granulosa Cells.}},
  author = {Xiaoliang Chen and Kang Zhang and Haipeng Feng and Lei Wang and Jingyan Zhang and Xiaorong Lu and Guowei Xu and Siqi Liu and Xiaowei Feng and Junyan Wang and Jianxi Li},
  journal = {Veterinary sciences},
  year = {2026},
  month = sep,
  doi = {10.3390/vetsci13101002},
  url = {https://europepmc.org/article/MED/42944098},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Mechanistic Investigation of Cryptotanshinone and Curcumin Combination in Attenuating BVDV-1-Induced Injury in Bovine Ovarian Granulosa Cells.
AU  - Xiaoliang Chen
AU  - Kang Zhang
AU  - Haipeng Feng
AU  - Lei Wang
AU  - Jingyan Zhang
AU  - Xiaorong Lu
AU  - Guowei Xu
AU  - Siqi Liu
AU  - Xiaowei Feng
AU  - Junyan Wang
AU  - Jianxi Li
PY  - 2026
DA  - 2026/09/23
JO  - Veterinary sciences
DO  - 10.3390/vetsci13101002
UR  - https://europepmc.org/article/MED/42944098
AB  - Bovine viral diarrhea virus (BVDV) is a widespread pathogen causing significant economic losses in cattle. There is an urgent need for drugs to treat BVDV-induced reproductive dysfunction. This study investigated the mechanisms and targets of the cryptotanshinone-curcumin (CRY-CUR) combination against BVDV-1. Overlapping targets of CRY, CUR, and BVDV were screened using TCMSP, ChEMBL, and GeneCards databases. A Venn diagram was generated, followed by GO and KEGG enrichment analyses via DAVID. A PPI network was constructed using Cytoscape, and molecular docking was performed with AutoDock. Anti-BVDV-1 activity was verified in bovine ovarian granulosa cells (BOGCs) using RT-qPCR and immunofluorescence. A total of 108 overlapping targets were identified, with four core targets (STAT3, TNF, BCL-2, and EGFR) selected. Molecular docking showed strong binding affinities. Enrichment analysis indicated involvement in calcium ion binding, apoptosis, and TNF/NF-κB pathways. In vitro, the CRY-CUR combination suppressed BVDV-1 replication and reversed abnormal mRNA expression of the core targets. The CRY-CUR combination may inhibit BVDV-1 replication, potentially through modulation of BVDV-1-related targets such as STAT3, TNF, BCL-2, and EGFR and their associated signaling pathways.
SN  - 2306-7381
ER  -