Skip to content
DraconiticResearch
Search Sources API
194,161 studies31 sourcesUpdated 45 min ago
PreprintCC BY-NCbioRxiv

Collagen staining with fast green FCF enables 3D imaging of pulmonary fibrosis

  • M. Saqib
  • A. K. Rivers
  • S. Masala
  • J. R. Baker
  • C. Hobbs
  • A. Boden
  • A. A. Jose
  • D. Herzog
  • S. J. Cleary1
  1. 1King's College London
Read full textPDFDOICite

Abstract

Current approaches for imaging fibrotic remodeling have sensitivity, specificity and cost drawbacks that limit both preclinical research and clinical diagnosis. Here, we show that fast green FCF, a small molecule that binds to fibrillar collagen, enables highly sensitive and specific imaging of fibrosis in lung samples from mice and humans using fluorescence microscopy. We report strategies for using fast green FCF staining to assess fibrotic remodeling using precision-cut lung slice and whole-biopsy preparations. Our findings demonstrate that fluorescence imaging of fast green FCF-stained collagen will be useful for fibrosis research and may help to improve detection of fibrosis in clinical pathology.

Related

  1. A Case of Autosomal Dominant Alport Syndrome with Normal Type IV Collagen α5 Staining Diagnosed by a Genetic Analysis
    Fuya Tanno, Yoichi Iwafuchi, Naofumi Imai +3
    Internal MedicinePeer-reviewedCC BY-NC-NDFull textGeneral medicineJ-STAGE
  2. Anatomopathological Aspects and Clinical Correlation of Conjunctival Bleb Fibrosis Following Encapsulation of a Biodegradable Collagen Matrix Implant in Glaucoma Surgery
    Luisa Castro-Roger, Sara Labay-Tejado, Xavier Suárez +2
    Turkish Journal of OphthalmologyPeer-reviewedCC BY-NC-ND 4.0Full textGeneral medicinePMCEurope PMC
  3. Therapeutic Targeting of Hepatic Stellate Cell Inactivation and Collagen Degradation by IC-2 Mitigates Liver Fibrosis
    Noriko Itaba, Ken-ichiro Abe, Yohei Kono +5
    International Journal of Molecular SciencesPeer-reviewedCC BY 4.0General medicineCrossref
  4. Remote ischemic conditioning mitigates diesel exhaust-induced pulmonary fibrosis by alleviating pulmonary inflammation and fibrogenesis
    Swati Kumari, Kaushlendra Mishra, Kanika Singh +8
    Scientific ReportsPeer-reviewedCC BY-NC-ND 4.0Full textGeneral medicineCrossref
  5. Urinary collagen type I degradation products as common fibrosis biomarkers in chronic diseases
    I. K. Mina, Y. Hussain, J. Siwy +14
    medRxivPreprintCC BYFull textGeneral medicinemedRxiv

Cite

BibTeXRIS
BibTeX
@misc{saqib2026collagen,
  title = {{Collagen staining with fast green FCF enables 3D imaging of pulmonary fibrosis}},
  author = {M. Saqib and A. K. Rivers and S. Masala and J. R. Baker and C. Hobbs and A. Boden and A. A. Jose and D. Herzog and S. J. Cleary},
  howpublished = {bioRxiv},
  year = {2026},
  month = aug,
  doi = {10.64898/2026.08.27.747478},
  url = {https://www.biorxiv.org/content/10.64898/2026.08.27.747478v1},
  note = {CC BY-NC},
}
RIS
TY  - UNPB
TI  - Collagen staining with fast green FCF enables 3D imaging of pulmonary fibrosis
AU  - M. Saqib
AU  - A. K. Rivers
AU  - S. Masala
AU  - J. R. Baker
AU  - C. Hobbs
AU  - A. Boden
AU  - A. A. Jose
AU  - D. Herzog
AU  - S. J. Cleary
PY  - 2026
DA  - 2026/08/27
PB  - bioRxiv
DO  - 10.64898/2026.08.27.747478
UR  - https://www.biorxiv.org/content/10.64898/2026.08.27.747478v1
AB  - Current approaches for imaging fibrotic remodeling have sensitivity, specificity and cost drawbacks that limit both preclinical research and clinical diagnosis. Here, we show that fast green FCF, a small molecule that binds to fibrillar collagen, enables highly sensitive and specific imaging of fibrosis in lung samples from mice and humans using fluorescence microscopy. We report strategies for using fast green FCF staining to assess fibrotic remodeling using precision-cut lung slice and whole-biopsy preparations. Our findings demonstrate that fluorescence imaging of fast green FCF-stained collagen will be useful for fibrosis research and may help to improve detection of fibrosis in clinical pathology.
ER  -