13C/31P MRS Metabolic Biomarkers of Disease Progression and Response to AAV Delivery of hGAA in a Mouse Model of Pompe Disease
Abstract
The development of therapeutic clinical trials for glycogen storage disorders, including Pompe disease, has called for non-invasive and objective biomarkers. Glycogen accumulation can be measured in vivo with 13 C MRS. However, clinical implementation remains challenging due to low signal-to-noise. On the other hand, the buildup of glycolytic intermediates may be detected with 31 P MRS. We sought to identify new biomarkers of disease progression in muscle using 13 C/ 31 P MRS and 1 H HR-MAS in a mouse model of Pompe disease ( Gaa −/− ). We evaluated the sensitivity of these MR biomarkers in vivo after treatment using an adeno-associated virus vector 2/9 encoding hGAA driven by the desmin promotor. 31 P MRS showed significantly elevated phosphomonoesters (PMEs) in Gaa −/− compared to control at 2 (0.06 ± 0.02 versus 0.03 ± 0.01; p = 0.003), 6, 12, and 18 months of age. Correlative 1 H HR-MAS measures in intact gastrocnemius muscles revealed high glucose-6-phosphate (G-6-P). After intramuscular AAV injections, glycogen, PME, and G-6-P were decreased within normal range. The changes in PME levels likely partly resulted from changes in G-6-P, one of the overlapping phosphomonoesters in the 31 P MR spectra in vivo. Because 31 P MRS is inherently more sensitive than 13 C MRS, PME levels have greater potential as a clinical biomarker and should be considered as a complementary approach for future studies in Pompe patients.
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BibTeX
@article{baligand202613c31p,
title = {{13C/31P MRS Metabolic Biomarkers of Disease Progression and Response to AAV Delivery of hGAA in a Mouse Model of Pompe Disease}},
author = {Celine Baligand and Adrian Todd and Brittany Lee-Mcmullen and Ravneet Vohra and Barry Byrne and Darin Falk and Glenn Walter},
journal = {Molecular Therapy - Methods and Clinical Development},
year = {2026},
month = oct,
doi = {10.1016/j.omtm.2017.09.002},
url = {https://hal.science/hal-05780238v1},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - 13C/31P MRS Metabolic Biomarkers of Disease Progression and Response to AAV Delivery of hGAA in a Mouse Model of Pompe Disease
AU - Celine Baligand
AU - Adrian Todd
AU - Brittany Lee-Mcmullen
AU - Ravneet Vohra
AU - Barry Byrne
AU - Darin Falk
AU - Glenn Walter
PY - 2026
DA - 2026/10/06
JO - Molecular Therapy - Methods and Clinical Development
DO - 10.1016/j.omtm.2017.09.002
UR - https://hal.science/hal-05780238v1
AB - The development of therapeutic clinical trials for glycogen storage disorders, including Pompe disease, has called for non-invasive and objective biomarkers. Glycogen accumulation can be measured in vivo with 13 C MRS. However, clinical implementation remains challenging due to low signal-to-noise. On the other hand, the buildup of glycolytic intermediates may be detected with 31 P MRS. We sought to identify new biomarkers of disease progression in muscle using 13 C/ 31 P MRS and 1 H HR-MAS in a mouse model of Pompe disease ( Gaa −/− ). We evaluated the sensitivity of these MR biomarkers in vivo after treatment using an adeno-associated virus vector 2/9 encoding hGAA driven by the desmin promotor. 31 P MRS showed significantly elevated phosphomonoesters (PMEs) in Gaa −/− compared to control at 2 (0.06 ± 0.02 versus 0.03 ± 0.01; p = 0.003), 6, 12, and 18 months of age. Correlative 1 H HR-MAS measures in intact gastrocnemius muscles revealed high glucose-6-phosphate (G-6-P). After intramuscular AAV injections, glycogen, PME, and G-6-P were decreased within normal range. The changes in PME levels likely partly resulted from changes in G-6-P, one of the overlapping phosphomonoesters in the 31 P MR spectra in vivo. Because 31 P MRS is inherently more sensitive than 13 C MRS, PME levels have greater potential as a clinical biomarker and should be considered as a complementary approach for future studies in Pompe patients.
SN - 2329-0501
ER -