An in vivo chemical genetic approach for targeted glycoproteome analysis in Drosophila melanogaster
- 1TUD Dresden University of Technology
Abstract
Glycans impact every aspect of physiology. Despite the rapid increase in relevance and translational potential, there remains a lack of tools for studying glycosylation in vivo. Here, we present FlyMOE, a chemical-genetic platform for targeted glycoprotein profiling in Drosophila melanogaster. Transgenic flies are equipped with biosynthetic capabilities to install bioorthogonally-tagged monosaccharides into the cellular glycoproteome. The genetic tractability of Drosophila allows for versatile application based on developmental stages, genetic drivers and glycosyltransferase enzymes. We employ FlyMOE to create an overview of the glycoproteome of Drosophila tissues and embryos based on mass spectrometry glycoproteomics, and investigate the substrates of individual members of the PGANT glycosyltransferase family in vivo. FlyMOE will allow the detailed investigation of the glycoproteome in Drosophila and similarly genetically modifiable in vivo model systems.
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BibTeX
@misc{schmidt2026vivo,
title = {{An in vivo chemical genetic approach for targeted glycoproteome analysis in Drosophila melanogaster}},
author = {S. D. Schmidt and C. Alexandre and L. Di Vagno and L. Zhang and H. Flynn and J. Kurth and S. Murray and S. Ruiz Herrera and H. Shaw and G. Bineva-Todd and O. Yilmaz Tastan and M. Skehel and N. L. Samara and K. Ten Hagen and J.-P. Vincent and B. Schumann},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.15.751681},
url = {https://www.biorxiv.org/content/10.64898/2026.09.15.751681v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - An in vivo chemical genetic approach for targeted glycoproteome analysis in Drosophila melanogaster
AU - S. D. Schmidt
AU - C. Alexandre
AU - L. Di Vagno
AU - L. Zhang
AU - H. Flynn
AU - J. Kurth
AU - S. Murray
AU - S. Ruiz Herrera
AU - H. Shaw
AU - G. Bineva-Todd
AU - O. Yilmaz Tastan
AU - M. Skehel
AU - N. L. Samara
AU - K. Ten Hagen
AU - J.-P. Vincent
AU - B. Schumann
PY - 2026
DA - 2026/09/15
PB - bioRxiv
DO - 10.64898/2026.09.15.751681
UR - https://www.biorxiv.org/content/10.64898/2026.09.15.751681v1
AB - Glycans impact every aspect of physiology. Despite the rapid increase in relevance and translational potential, there remains a lack of tools for studying glycosylation in vivo. Here, we present FlyMOE, a chemical-genetic platform for targeted glycoprotein profiling in Drosophila melanogaster. Transgenic flies are equipped with biosynthetic capabilities to install bioorthogonally-tagged monosaccharides into the cellular glycoproteome. The genetic tractability of Drosophila allows for versatile application based on developmental stages, genetic drivers and glycosyltransferase enzymes. We employ FlyMOE to create an overview of the glycoproteome of Drosophila tissues and embryos based on mass spectrometry glycoproteomics, and investigate the substrates of individual members of the PGANT glycosyltransferase family in vivo. FlyMOE will allow the detailed investigation of the glycoproteome in Drosophila and similarly genetically modifiable in vivo model systems.
ER -