Development and Optimization of a Molecularly Imprinted Polymer for Electrochemical Detection of Escherichia coli
- 1Department of Biomedical Engineering, School of Engineering, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
- 2Department of Physics, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand
Abstract
In this study, a molecularly imprinted polymer (MIP)-based electrochemical biosensor was developed for the selective detection of Escherichia coli (E. coli). The MIP recognition layer was synthesized by free-radical polymerization using MAM, AAM and VP as functional monomers with E. coli cells as the imprinting template. The MIP was immobilized onto a screen-printed electrode and evaluated using cyclic voltammetry (CV). Six MIP formulations with different MAM:AAM ratios were investigated to determine the optimal monomer composition. The optimized formulation, with an MAM:AAM ratio of 2:3:1, was evaluated over an E. coli concentration range of 10–105 CFU/mL and exhibited a linear response with a slope of 4.1107 and an R2 value of 0.9893 with a detection limit of 1.52 CFU/mL. The selectivity of the optimized sensor was further evaluated using Enterococcus faecalis and Pseudomonas aeruginosa, which showed lower responses than E. coli. These results indicate that the proposed MIP-based electrochemical biosensor provides preferential recognition of E. coli under the investigated conditions and demonstrates potential for bacterial detection.
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BibTeX
@article{pintavirooj2026development,
title = {{Development and Optimization of a Molecularly Imprinted Polymer for Electrochemical Detection of Escherichia coli}},
author = {Chuchart Pintavirooj and Naphatsawan Vongmanee and Sarinporn Visitsattapongse},
journal = {Polymers},
year = {2026},
month = oct,
doi = {10.3390/polym18202508},
url = {https://www.mdpi.com/2073-4360/18/20/2508},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Development and Optimization of a Molecularly Imprinted Polymer for Electrochemical Detection of Escherichia coli
AU - Chuchart Pintavirooj
AU - Naphatsawan Vongmanee
AU - Sarinporn Visitsattapongse
PY - 2026
DA - 2026/10/14
JO - Polymers
DO - 10.3390/polym18202508
UR - https://www.mdpi.com/2073-4360/18/20/2508
AB - In this study, a molecularly imprinted polymer (MIP)-based electrochemical biosensor was developed for the selective detection of Escherichia coli (E. coli). The MIP recognition layer was synthesized by free-radical polymerization using MAM, AAM and VP as functional monomers with E. coli cells as the imprinting template. The MIP was immobilized onto a screen-printed electrode and evaluated using cyclic voltammetry (CV). Six MIP formulations with different MAM:AAM ratios were investigated to determine the optimal monomer composition. The optimized formulation, with an MAM:AAM ratio of 2:3:1, was evaluated over an E. coli concentration range of 10–105 CFU/mL and exhibited a linear response with a slope of 4.1107 and an R2 value of 0.9893 with a detection limit of 1.52 CFU/mL. The selectivity of the optimized sensor was further evaluated using Enterococcus faecalis and Pseudomonas aeruginosa, which showed lower responses than E. coli. These results indicate that the proposed MIP-based electrochemical biosensor provides preferential recognition of E. coli under the investigated conditions and demonstrates potential for bacterial detection.
SN - 2073-4360
ER -