Genome-wide analysis of the rice phloem protein 2 gene family identifies OsPP2–10 and OsPP2–13 as candidate genes associated with salt and osmotic stress responses
- 1Biotechnology Research Institute, Chongqing Academy of Agricultural Sciences
- 2Chongqing Key Laboratory of Adversity Agriculture Research
- 3Key Laboratory of Evaluation and Utilization for Special Crops Germplasm Resource in the Southwest Mountains, Ministry of Agriculture and Rural Affairs
Abstract
Introduction: Phloem protein 2 (PP2) proteins are major structural components of the phloem and play important roles in long-distance signaling and plant responses to environmental stresses. However, the evolutionary history and potential functions of the PP2 gene family in rice remain poorly understood.
Methods: We identified 20 OsPP2 genes and analyzed their evolutionary relationships, gene structures, duplication events, promoter cis-regulatory elements, and expression profiles. Quantitative real-time PCR (qRT-PCR) was used to validate stress-responsive expression patterns. Subcellular localization and yeast heterologous expression assays were performed to further investigate the potential functions of OsPP2-10 and OsPP2-13 .
Results: Both segmental and tandem duplication contributed to the expansion of the OsPP2 family. Promoter analysis identified multiple predicted stress- and hormone-responsive cis-regulatory elements in most OsPP2 genes. Expression profiling revealed distinct tissue-specific patterns and differential responses to salt, drought, heat, and cold stresses. OsPP2-10 and OsPP2-13 showed relatively strong expression responses to salt stress and PEG6000-induced osmotic stress, consistent with the qRT-PCR results. Subcellular localization analysis detected both proteins in the nucleus and peripheral cellular regions. Heterologous expression of these genes enhanced yeast tolerance to salt and osmotic stress.
Discussion: These findings provide a systematic overview of the rice PP2 gene family and preliminary evidence supporting the potential involvement of OsPP2-10 and OsPP2-13 in abiotic stress responses. Both genes represent promising candidates for further functional investigation in rice.
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BibTeX
@article{dai2026genomewide,
title = {{Genome-wide analysis of the rice phloem protein 2 gene family identifies OsPP2–10 and OsPP2–13 as candidate genes associated with salt and osmotic stress responses}},
author = {Yongdong Dai and Yang Chen},
journal = {Frontiers in Plant Science},
year = {2026},
month = oct,
doi = {10.3389/fpls.2026.1953442},
url = {https://www.frontiersin.org/articles/10.3389/fpls.2026.1953442/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Genome-wide analysis of the rice phloem protein 2 gene family identifies OsPP2–10 and OsPP2–13 as candidate genes associated with salt and osmotic stress responses
AU - Yongdong Dai
AU - Yang Chen
PY - 2026
DA - 2026/10/21
JO - Frontiers in Plant Science
DO - 10.3389/fpls.2026.1953442
UR - https://www.frontiersin.org/articles/10.3389/fpls.2026.1953442/full
AB - Introduction: Phloem protein 2 (PP2) proteins are major structural components of the phloem and play important roles in long-distance signaling and plant responses to environmental stresses. However, the evolutionary history and potential functions of the PP2 gene family in rice remain poorly understood. Methods: We identified 20 OsPP2 genes and analyzed their evolutionary relationships, gene structures, duplication events, promoter cis-regulatory elements, and expression profiles. Quantitative real-time PCR (qRT-PCR) was used to validate stress-responsive expression patterns. Subcellular localization and yeast heterologous expression assays were performed to further investigate the potential functions of OsPP2-10 and OsPP2-13 . Results: Both segmental and tandem duplication contributed to the expansion of the OsPP2 family. Promoter analysis identified multiple predicted stress- and hormone-responsive cis-regulatory elements in most OsPP2 genes. Expression profiling revealed distinct tissue-specific patterns and differential responses to salt, drought, heat, and cold stresses. OsPP2-10 and OsPP2-13 showed relatively strong expression responses to salt stress and PEG6000-induced osmotic stress, consistent with the qRT-PCR results. Subcellular localization analysis detected both proteins in the nucleus and peripheral cellular regions. Heterologous expression of these genes enhanced yeast tolerance to salt and osmotic stress. Discussion: These findings provide a systematic overview of the rice PP2 gene family and preliminary evidence supporting the potential involvement of OsPP2-10 and OsPP2-13 in abiotic stress responses. Both genes represent promising candidates for further functional investigation in rice.
SN - 1664-462X
ER -