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Peer-reviewedCC BYThe Plant journal : for cell and molecular biology

The plant longevity gene AHL15 delays leaf senescence by repressing ORESARA1 and cytokinin degradation.

  • Thalia Luden1
  • Petra Amakorová2
  • Ellora Basu1
  • Ondřej Novák2
  • Salma Balazadeh3
  • Remko Offringa1
  1. 1Plant Developmental Genetics, Institute of Biology Leiden, Leiden University, Sylviusweg 72, Leiden, 2333 BE, the Netherlands.
  2. 2Laboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, The Czech Academy of Sciences, CZ-77900, Olomouc, Czech Republic.
  3. 3Plant Stress Biology, Institute of Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE, Leiden, the Netherlands.
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Abstract

The Arabidopsis thaliana (Arabidopsis) AT-HOOK MOTIF NUCLEAR LOCALIZED 15 (AHL15) gene is associated with various longevity phenotypes and extends the life span of plants when overexpressed. In this study, we show that, in addition to previously described longevity phenotypes, constitutive overexpression of AHL15 in Arabidopsis delays leaf senescence, whereas ahl15 loss of function accelerates this process. Dexamethasone-induced nuclear localization of AHL15-GR during dark-triggered senescence results in a stay-green phenotype and represses the expression of several early senescence-associated genes. Among these, the ORESARA1 (ORE1) locus is directly bound by AHL15, suggesting a direct delaying effect of AHL15 on senescence. Furthermore, we demonstrate that AHL15 acts by directly repressing the expression of several CYTOKININ OXIDASE (CKX) genes involved in cytokinin inactivation, resulting in delayed degradation of cytokinins during dark-induced senescence. Cytokinins are known to inhibit senescence, and this, together with the downregulation of ORE1 expression, explains the delaying effect of AHL15 on senescence.

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Cite

BibTeXRIS
BibTeX
@article{luden2026plant,
  title = {{The plant longevity gene AHL15 delays leaf senescence by repressing ORESARA1 and cytokinin degradation.}},
  author = {Thalia Luden and Petra Amakorová and Ellora Basu and Ondřej Novák and Salma Balazadeh and Remko Offringa},
  journal = {The Plant journal : for cell and molecular biology},
  year = {2026},
  month = oct,
  doi = {10.1111/tpj.71177},
  url = {https://europepmc.org/article/MED/42876059},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - The plant longevity gene AHL15 delays leaf senescence by repressing ORESARA1 and cytokinin degradation.
AU  - Thalia Luden
AU  - Petra Amakorová
AU  - Ellora Basu
AU  - Ondřej Novák
AU  - Salma Balazadeh
AU  - Remko Offringa
PY  - 2026
DA  - 2026/10/01
JO  - The Plant journal : for cell and molecular biology
DO  - 10.1111/tpj.71177
UR  - https://europepmc.org/article/MED/42876059
AB  - The Arabidopsis thaliana (Arabidopsis) AT-HOOK MOTIF NUCLEAR LOCALIZED 15 (AHL15) gene is associated with various longevity phenotypes and extends the life span of plants when overexpressed. In this study, we show that, in addition to previously described longevity phenotypes, constitutive overexpression of AHL15 in Arabidopsis delays leaf senescence, whereas ahl15 loss of function accelerates this process. Dexamethasone-induced nuclear localization of AHL15-GR during dark-triggered senescence results in a stay-green phenotype and represses the expression of several early senescence-associated genes. Among these, the ORESARA1 (ORE1) locus is directly bound by AHL15, suggesting a direct delaying effect of AHL15 on senescence. Furthermore, we demonstrate that AHL15 acts by directly repressing the expression of several CYTOKININ OXIDASE (CKX) genes involved in cytokinin inactivation, resulting in delayed degradation of cytokinins during dark-induced senescence. Cytokinins are known to inhibit senescence, and this, together with the downregulation of ORE1 expression, explains the delaying effect of AHL15 on senescence.
SN  - 0960-7412
SN  - 1365-313X
ER  -