Phenolic-Rich Aronia melanocarpa (cv. Nero) Extract Attenuates Doxorubicin-Induced Nephrotoxicity Associated with Reduced Oxidative Stress, Inflammation, and Endoplasmic Reticulum Stress
6 affiliations
- 1Vocational School of Health Sciences, Karadeniz Technical University, Trabzon 61080, Türkiye
- 2Department of Medical Biochemistry, Trabzon University, Trabzon 61080, Türkiye
- 3Department of Histology and Embryology, Faculty of Medicine, Karadeniz Technical University, Trabzon 61080, Türkiye
- 4Department of Chemistry, Faculty of Science, Karadeniz Technical University, Trabzon 61080, Türkiye
- 5Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon 61080, Türkiye
- 6Department of Biochemistry, Faculty of Medicine, Nakhchivan State University, Nakhchivan AZ7000, Azerbaijan
Abstract
Aronia melanocarpa (black chokeberry) is a polyphenol-rich berry recognized as a functional food because of its potent antioxidant properties. This study investigated the phenolic composition, antioxidant capacity, and nephroprotective effects of A. melanocarpa extract (AME) against doxorubicin (DXR)-induced renal injury. Antioxidant activity was evaluated by determining total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity. Thirty-six female Sprague–Dawley rats (180–220 g) were randomly allocated to four groups (n = 9 per group): Control, AME, DXR, and AME + DXR. AME (200 mg/kg/day) was administered orally for 21 consecutive days as a pretreatment, and acute nephrotoxicity was induced by a single intraperitoneal injection of DXR (20 mg/kg) on day 21. Samples were collected 24 h after DXR administration. Renal injury was evaluated using biochemical, histopathological, oxidative stress (OS), inflammatory, endoplasmic reticulum stress (ERS), and apoptosis-related parameters, together with TUNEL staining. HPLC–PDA analysis of 29 targeted phenolic compounds identified cya-nidin-3-galactoside (2440 µg/g FW), cyanidin-3-arabinoside (350 µg/g FW), chlorogenic acid (251.83 µg/g FW), and rutin (32.29 µg/g FW) as the major constituents. DXR markedly impaired renal function and increased oxidative stress, ERS, inflammation, and apoptosis, whereas AME pretreatment significantly attenuated these alterations. These findings are consistent with a protective effect of phenolic-rich AME against acute DXR-induced renal injury in rats, associated with attenuation of oxidative stress, inflammation, ERS, and apoptosis. Further studies are needed to confirm these findings and evaluate their potential clinical relevance.
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BibTeX
@article{malkoc2026phenolicrich,
title = {{Phenolic-Rich Aronia melanocarpa (cv. Nero) Extract Attenuates Doxorubicin-Induced Nephrotoxicity Associated with Reduced Oxidative Stress, Inflammation, and Endoplasmic Reticulum Stress}},
author = {Meltem Arıkan Malkoç and Serap Özer Yaman and Hüseyin Çınkılıç and Esin Yuluğ and Yakup Kara and Birgül Kural and Sevgi Kolaylı},
journal = {Molecules},
year = {2026},
month = oct,
doi = {10.3390/molecules31203732},
url = {https://www.mdpi.com/1420-3049/31/20/3732},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Phenolic-Rich Aronia melanocarpa (cv. Nero) Extract Attenuates Doxorubicin-Induced Nephrotoxicity Associated with Reduced Oxidative Stress, Inflammation, and Endoplasmic Reticulum Stress
AU - Meltem Arıkan Malkoç
AU - Serap Özer Yaman
AU - Hüseyin Çınkılıç
AU - Esin Yuluğ
AU - Yakup Kara
AU - Birgül Kural
AU - Sevgi Kolaylı
PY - 2026
DA - 2026/10/01
JO - Molecules
DO - 10.3390/molecules31203732
UR - https://www.mdpi.com/1420-3049/31/20/3732
AB - Aronia melanocarpa (black chokeberry) is a polyphenol-rich berry recognized as a functional food because of its potent antioxidant properties. This study investigated the phenolic composition, antioxidant capacity, and nephroprotective effects of A. melanocarpa extract (AME) against doxorubicin (DXR)-induced renal injury. Antioxidant activity was evaluated by determining total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity. Thirty-six female Sprague–Dawley rats (180–220 g) were randomly allocated to four groups (n = 9 per group): Control, AME, DXR, and AME + DXR. AME (200 mg/kg/day) was administered orally for 21 consecutive days as a pretreatment, and acute nephrotoxicity was induced by a single intraperitoneal injection of DXR (20 mg/kg) on day 21. Samples were collected 24 h after DXR administration. Renal injury was evaluated using biochemical, histopathological, oxidative stress (OS), inflammatory, endoplasmic reticulum stress (ERS), and apoptosis-related parameters, together with TUNEL staining. HPLC–PDA analysis of 29 targeted phenolic compounds identified cya-nidin-3-galactoside (2440 µg/g FW), cyanidin-3-arabinoside (350 µg/g FW), chlorogenic acid (251.83 µg/g FW), and rutin (32.29 µg/g FW) as the major constituents. DXR markedly impaired renal function and increased oxidative stress, ERS, inflammation, and apoptosis, whereas AME pretreatment significantly attenuated these alterations. These findings are consistent with a protective effect of phenolic-rich AME against acute DXR-induced renal injury in rats, associated with attenuation of oxidative stress, inflammation, ERS, and apoptosis. Further studies are needed to confirm these findings and evaluate their potential clinical relevance.
SN - 1420-3049
ER -