Annexin 5 is a key player in orchestrating the pollen sequestrome
- 1Institute of Biochemistry and Biophysics PAS, Warsaw, Poland
Abstract
Like dry seeds, mature pollen grains store a large reservoir of RNA species that enables rapid activation and germination under favorable conditions. The molecular mechanisms governing the accumulation of these RNA reservoirs remain poorly understood. Here, we show that the Arabidopsis pollen-specific protein annexin 5 (ANN5) localizes to the nucleolus and exerts dose-dependent control over ribosome biogenesis, as evidenced by altered steady-state rRNA levels in mature pollen. Ectopic expression of ANN5 also induced the accumulation of translationally repressed RNA in plant somatic tissues. Mechanistically, ANN5 interacts with histone deacetylase 2B (HD2B) and nucleolin (NUC1), which are known epigenetic modulators of ribosome biogenesis. In metabolically active cells, ANN5 associates with ribosomal and ribosome-associated proteins, suggesting it modulates the regulatory landscape of translation. We propose that the primary physiological role of ANN5 is to coordinate the formation and subsequent availability of RNA reserves during pollen maturation and activation.
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BibTeX
@misc{lichocka2026annexin,
title = {{Annexin 5 is a key player in orchestrating the pollen sequestrome}},
author = {M. Lichocka and A. Fiodor and O. Iwanska and A. Golisz-Mocydlarz and M. Zakrzewska-Placzek and M. Gorecka and M. Grech-Baran and S. Kubala and A. Anielska-Mazur and N. Lewkowicz and I. Barymow-Filoniuk and A. L. Starosta and J. Hennig and M. Krzymowska},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.22.760708},
url = {https://www.biorxiv.org/content/10.64898/2026.10.22.760708v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Annexin 5 is a key player in orchestrating the pollen sequestrome
AU - M. Lichocka
AU - A. Fiodor
AU - O. Iwanska
AU - A. Golisz-Mocydlarz
AU - M. Zakrzewska-Placzek
AU - M. Gorecka
AU - M. Grech-Baran
AU - S. Kubala
AU - A. Anielska-Mazur
AU - N. Lewkowicz
AU - I. Barymow-Filoniuk
AU - A. L. Starosta
AU - J. Hennig
AU - M. Krzymowska
PY - 2026
DA - 2026/10/22
PB - bioRxiv
DO - 10.64898/2026.10.22.760708
UR - https://www.biorxiv.org/content/10.64898/2026.10.22.760708v1
AB - Like dry seeds, mature pollen grains store a large reservoir of RNA species that enables rapid activation and germination under favorable conditions. The molecular mechanisms governing the accumulation of these RNA reservoirs remain poorly understood. Here, we show that the Arabidopsis pollen-specific protein annexin 5 (ANN5) localizes to the nucleolus and exerts dose-dependent control over ribosome biogenesis, as evidenced by altered steady-state rRNA levels in mature pollen. Ectopic expression of ANN5 also induced the accumulation of translationally repressed RNA in plant somatic tissues. Mechanistically, ANN5 interacts with histone deacetylase 2B (HD2B) and nucleolin (NUC1), which are known epigenetic modulators of ribosome biogenesis. In metabolically active cells, ANN5 associates with ribosomal and ribosome-associated proteins, suggesting it modulates the regulatory landscape of translation. We propose that the primary physiological role of ANN5 is to coordinate the formation and subsequent availability of RNA reserves during pollen maturation and activation.
ER -