Isolation to purification: An integrated study on lysine production by Bacillus megaterium NTJT4 from medicinal plant rhizosphere
- 1Institute of Technology Transfer and Innovation (ITTI), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh.
Abstract
L-lysine stands out as a commercially important amino acid vital for maintaining balanced nutrition, immune function and healthy development in humans and animals. As global demand for lysine continues to rise each year, large-scale production is required to keep pace with this growing need. In the present study, L-lysine production was accomplished through a fermentation process utilizing isolated bacterial strains from soil. After conducting primary screening, Bacillus megaterium NTJT4 was identified as the highest lysine producer. Various fermentation parameters including fermentation time, pH, temperature, different carbon and nitrogen sources, glucose levels, ammonium sulfate concentration, growth stimulators, and vitamin concentrations were evaluated to optimize lysine production. The study found that the optimal conditions for lysine production were 72 hours, pH 7.0, and 37 °C. The effect of different carbon and nitrogen sources showed that 8% glucose and 1% ammonium sulfate resulted in increased lysine production (4.86 ± 0.20 mg/mL and 3.95 ± 0.14 mg/mL respectively). Media with combination of peptone and yeast extract as growth stimulators showed increased lysine yield (4.86 ± 0.31 mg/mL) and addition of 100 µg/mL thiamine-HCL as a source of vitamin resulted in increased lysine yield (5.37 ± 0.16 mg/mL). Amberlite IR‑120–based ion‑exchange chromatography achieved 100.0% purity and 58.0% recovery of lysine. The purified lysine concentration reached 13.44 mg/mL with a purification fold of 3.85. These results indicate that the newly isolated Bacillus megaterium NTJT4 possesses considerable potential for diverse biotechnological and industrial applications, particularly in microbial amino acid production.
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BibTeX
@article{jannat2026isolation,
title = {{Isolation to purification: An integrated study on lysine production by Bacillus megaterium NTJT4 from medicinal plant rhizosphere}},
author = {Taslimul Jannat and Md Rezaul Karim and Md Rakibul Hasan and Debabrata Karmakar and Nishat Tasnim and Nourin Tarannum},
journal = {PLOS One},
year = {2026},
month = oct,
doi = {10.1371/journal.pone.0361763},
url = {https://doi.org/10.1371/journal.pone.0361763},
note = {CC BY},
}
RIS
TY - JOUR
TI - Isolation to purification: An integrated study on lysine production by Bacillus megaterium NTJT4 from medicinal plant rhizosphere
AU - Taslimul Jannat
AU - Md Rezaul Karim
AU - Md Rakibul Hasan
AU - Debabrata Karmakar
AU - Nishat Tasnim
AU - Nourin Tarannum
PY - 2026
DA - 2026/10/30
JO - PLOS One
DO - 10.1371/journal.pone.0361763
UR - https://doi.org/10.1371/journal.pone.0361763
AB - L-lysine stands out as a commercially important amino acid vital for maintaining balanced nutrition, immune function and healthy development in humans and animals. As global demand for lysine continues to rise each year, large-scale production is required to keep pace with this growing need. In the present study, L-lysine production was accomplished through a fermentation process utilizing isolated bacterial strains from soil. After conducting primary screening, Bacillus megaterium NTJT4 was identified as the highest lysine producer. Various fermentation parameters including fermentation time, pH, temperature, different carbon and nitrogen sources, glucose levels, ammonium sulfate concentration, growth stimulators, and vitamin concentrations were evaluated to optimize lysine production. The study found that the optimal conditions for lysine production were 72 hours, pH 7.0, and 37 °C. The effect of different carbon and nitrogen sources showed that 8% glucose and 1% ammonium sulfate resulted in increased lysine production (4.86 ± 0.20 mg/mL and 3.95 ± 0.14 mg/mL respectively). Media with combination of peptone and yeast extract as growth stimulators showed increased lysine yield (4.86 ± 0.31 mg/mL) and addition of 100 µg/mL thiamine-HCL as a source of vitamin resulted in increased lysine yield (5.37 ± 0.16 mg/mL). Amberlite IR‑120–based ion‑exchange chromatography achieved 100.0% purity and 58.0% recovery of lysine. The purified lysine concentration reached 13.44 mg/mL with a purification fold of 3.85. These results indicate that the newly isolated Bacillus megaterium NTJT4 possesses considerable potential for diverse biotechnological and industrial applications, particularly in microbial amino acid production.
SN - 1932-6203
ER -