Same-day hybrid capture workflow for diagnosis and surveillance of respiratory pathogens
Abstract
Hybrid capture approaches can detect a broad range of pathogens directly from clinical samples with greater sensitivity than untargeted approaches. Clinical adoption has been limited by long turnaround times, complex workflows, and perceived high costs. We iteratively developed and validated a rapid hybrid capture workflow based on Illuminas RPIP protocol (standard workflow). Rapid-RPIP v1 reduced wet-lab time from [~]11.4 to [~]7.3 h, for a turnaround time (TAT) of <15 h. Rapid-RPIP v2 further reduced wet-lab time by [~]0.7 h and used Illumina Turbo Sequencing shortening the sequencing time from [~]5.5 to [~]2.5 h thus achieving a TAT of <10 h without compromising sensitivity and yielding higher RPKMs for RNA viruses than the standard workflow. This approach could be readily adapted to a broad range of clinical and public health applications.
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BibTeX
@misc{buggiotti2026sameday,
title = {{Same-day hybrid capture workflow for diagnosis and surveillance of respiratory pathogens}},
author = {L. Buggiotti and A. Busam and L. M. Martin Bernal and T. Brooks and S. Buddle and A. Kopec and L. Ahmed and G. Kuczmar and A. Lennon and S. Castellano and P. Simmonds and H. Harvala and T. Golubchik and J. R. Brown and R. Williams and J. Breuer and S. Morfopoulou and O. E. Torres Monteguth},
howpublished = {medRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.09.26365131},
url = {https://www.medrxiv.org/content/10.64898/2026.10.09.26365131v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Same-day hybrid capture workflow for diagnosis and surveillance of respiratory pathogens
AU - L. Buggiotti
AU - A. Busam
AU - L. M. Martin Bernal
AU - T. Brooks
AU - S. Buddle
AU - A. Kopec
AU - L. Ahmed
AU - G. Kuczmar
AU - A. Lennon
AU - S. Castellano
AU - P. Simmonds
AU - H. Harvala
AU - T. Golubchik
AU - J. R. Brown
AU - R. Williams
AU - J. Breuer
AU - S. Morfopoulou
AU - O. E. Torres Monteguth
PY - 2026
DA - 2026/10/09
PB - medRxiv
DO - 10.64898/2026.10.09.26365131
UR - https://www.medrxiv.org/content/10.64898/2026.10.09.26365131v1
AB - Hybrid capture approaches can detect a broad range of pathogens directly from clinical samples with greater sensitivity than untargeted approaches. Clinical adoption has been limited by long turnaround times, complex workflows, and perceived high costs. We iteratively developed and validated a rapid hybrid capture workflow based on Illuminas RPIP protocol (standard workflow). Rapid-RPIP v1 reduced wet-lab time from [~]11.4 to [~]7.3 h, for a turnaround time (TAT) of <15 h. Rapid-RPIP v2 further reduced wet-lab time by [~]0.7 h and used Illumina Turbo Sequencing shortening the sequencing time from [~]5.5 to [~]2.5 h thus achieving a TAT of <10 h without compromising sensitivity and yielding higher RPKMs for RNA viruses than the standard workflow. This approach could be readily adapted to a broad range of clinical and public health applications.
ER -