Cumulative cgMLST provides increased discrimination of nested phylogenetic groups.
- 1Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, Paris, France.
- 2Multidrug-Resistant Organism Repository and Surveillance Network (MRSN), Diagnostics and Countermeasures Branch, CIDR, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
- 3Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, Paris, France. [email protected].
Abstract
Background
Core genome multilocus sequence typing (cgMLST) is a powerful method for bacterial strain genotyping. However, the size of the core genome decreases as the phylogenetic breadth of the target group increases, reducing discriminatory power. To overcome this discrimination/applicability tradeoff, here we developed a cumulative cgMLST approach, where sets of core loci conserved within nested phylogenetic entities are added. We illustrate this approach using the Klebsiella pneumoniae species complex (KpSC), for which a widely used cgMLST scheme (KpSC-cgMLST) comprises only 629 genes.Methods
We created non-redundant cgMLST schemes for the individual species K. pneumoniae sensu stricto (Kpn-cgMLST scheme), and its multidrug resistant sublineages (SLs) SL147 and SL307. To extract core genes, we used 37,874 genome assemblies originating from over 80 countries worldwide. A methodology was set to filter redundant loci before importing them into the genotyping tool BIGSdb, where they were combined into schemes together with preexisting loci conserved at higher phylogenetic levels. The performance of the cumulative cgMLST schemes was evaluated on previously published datasets and on novel data from an inter-hospital outbreak of SL307.Results
The Kpn-cgMLST, SL147 and SL307 schemes comprise 2752, 852, and 947 additional loci, respectively. The mean allele call rate of the novel loci was > 99% in the validation datasets. Compared to the KpSC scheme used alone, pairwise allelic distances among isolates increased on average 5.6-fold using the Kpn scheme, and further by 1.2-fold and 1.3-fold using the SL147 and SL307 schemes, respectively. We demonstrated the added value of this increased discriminatory power for epidemiological analyses and observed a level of discrimination that approaches that of whole-genome single nucleotide polymorphism analysis.Conclusions
The cumulative cgMLST strategy combines broad phylogenetic applicability and nearly complete genotyping resolution, expanding the utility of this harmonized approach for genomic epidemiology.
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BibTeX
@article{ovsepian2026cumulative,
title = {{Cumulative cgMLST provides increased discrimination of nested phylogenetic groups.}},
author = {Armen Ovsepian and Jose F Delgado-Blas and Martin Rethoret-Pasty and Melissa J Martin and François Lebreton and Sylvain Brisse},
journal = {Genome medicine},
year = {2026},
month = jul,
doi = {10.1186/s13073-026-01720-z},
url = {https://europepmc.org/article/MED/42897578},
note = {CC BY},
}
RIS
TY - JOUR
TI - Cumulative cgMLST provides increased discrimination of nested phylogenetic groups.
AU - Armen Ovsepian
AU - Jose F Delgado-Blas
AU - Martin Rethoret-Pasty
AU - Melissa J Martin
AU - François Lebreton
AU - Sylvain Brisse
PY - 2026
DA - 2026/07/01
JO - Genome medicine
DO - 10.1186/s13073-026-01720-z
UR - https://europepmc.org/article/MED/42897578
AB - Background Core genome multilocus sequence typing (cgMLST) is a powerful method for bacterial strain genotyping. However, the size of the core genome decreases as the phylogenetic breadth of the target group increases, reducing discriminatory power. To overcome this discrimination/applicability tradeoff, here we developed a cumulative cgMLST approach, where sets of core loci conserved within nested phylogenetic entities are added. We illustrate this approach using the Klebsiella pneumoniae species complex (KpSC), for which a widely used cgMLST scheme (KpSC-cgMLST) comprises only 629 genes.Methods We created non-redundant cgMLST schemes for the individual species K. pneumoniae sensu stricto (Kpn-cgMLST scheme), and its multidrug resistant sublineages (SLs) SL147 and SL307. To extract core genes, we used 37,874 genome assemblies originating from over 80 countries worldwide. A methodology was set to filter redundant loci before importing them into the genotyping tool BIGSdb, where they were combined into schemes together with preexisting loci conserved at higher phylogenetic levels. The performance of the cumulative cgMLST schemes was evaluated on previously published datasets and on novel data from an inter-hospital outbreak of SL307.Results The Kpn-cgMLST, SL147 and SL307 schemes comprise 2752, 852, and 947 additional loci, respectively. The mean allele call rate of the novel loci was > 99% in the validation datasets. Compared to the KpSC scheme used alone, pairwise allelic distances among isolates increased on average 5.6-fold using the Kpn scheme, and further by 1.2-fold and 1.3-fold using the SL147 and SL307 schemes, respectively. We demonstrated the added value of this increased discriminatory power for epidemiological analyses and observed a level of discrimination that approaches that of whole-genome single nucleotide polymorphism analysis.Conclusions The cumulative cgMLST strategy combines broad phylogenetic applicability and nearly complete genotyping resolution, expanding the utility of this harmonized approach for genomic epidemiology.
SN - 1756-994X
ER -