Evaluation of BCR::ABL1 Positivity in Peripheral Blood Neutrophils Enables the Diagnosis of De Novo Blast Phase Chronic Myeloid Leukemia with Additional Structural Abnormalities of the Philadelphia Chromosome
Abstract
A 60-year-old man with no history of hematologic disease presented with leukocytosis and thrombocytopenia. A bone marrow (BM) examination revealed 85.0% blasts with a B/myeloid immunophenotype and a complex Philadelphia (Ph) chromosome-positive karyotype: 46,XY,t(9;22)(q34;q11.2)[3]/45,XY,der(9)t(9;22),add(19)(p13),-22[17]. Fluorescence in situ hybridization detected single (6%) or duplicated (92%) BCR::ABL1 signals in bone marrow (BM) blasts but only single BCR::ABL1 signals (95%) in peripheral blood (PB) neutrophils. These findings indicate that the duplicated-signal blasts arrested their differentiation, while single-signal blasts retained differentiation potential, supporting a diagnosis of de novo blast phase chronic myeloid leukemia. An evaluation of BCR::ABL1 positivity in PB neutrophils provides valuable diagnostic insights into Philadelphia chromosome-positive leukemias.
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BibTeX
@article{sakai2026evaluation,
title = {{Evaluation of BCR::ABL1 Positivity in Peripheral Blood Neutrophils Enables the Diagnosis of De Novo Blast Phase Chronic Myeloid Leukemia with Additional Structural Abnormalities of the Philadelphia Chromosome}},
author = {Hirotaka Sakai and Ikuo Miura and Ayako Arai},
journal = {Internal Medicine},
year = {2026},
month = sep,
doi = {10.2169/internalmedicine.7790-26},
url = {https://www.jstage.jst.go.jp/article/internalmedicine/advpub/0/advpub_7790-26/_article},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Evaluation of BCR::ABL1 Positivity in Peripheral Blood Neutrophils Enables the Diagnosis of De Novo Blast Phase Chronic Myeloid Leukemia with Additional Structural Abnormalities of the Philadelphia Chromosome
AU - Hirotaka Sakai
AU - Ikuo Miura
AU - Ayako Arai
PY - 2026
DA - 2026/09/01
JO - Internal Medicine
DO - 10.2169/internalmedicine.7790-26
UR - https://www.jstage.jst.go.jp/article/internalmedicine/advpub/0/advpub_7790-26/_article
AB - A 60-year-old man with no history of hematologic disease presented with leukocytosis and thrombocytopenia. A bone marrow (BM) examination revealed 85.0% blasts with a B/myeloid immunophenotype and a complex Philadelphia (Ph) chromosome-positive karyotype: 46,XY,t(9;22)(q34;q11.2)[3]/45,XY,der(9)t(9;22),add(19)(p13),-22[17]. Fluorescence in situ hybridization detected single (6%) or duplicated (92%) BCR::ABL1 signals in bone marrow (BM) blasts but only single BCR::ABL1 signals (95%) in peripheral blood (PB) neutrophils. These findings indicate that the duplicated-signal blasts arrested their differentiation, while single-signal blasts retained differentiation potential, supporting a diagnosis of de novo blast phase chronic myeloid leukemia. An evaluation of BCR::ABL1 positivity in PB neutrophils provides valuable diagnostic insights into Philadelphia chromosome-positive leukemias.
SN - 1349-7235
SN - 0918-2918
ER -