High-resolution holographic reconstruction combined with fluorescence microscopy to identify unwanted
- 1Department of Chemical Engineering, University of Almeria, 04120, Almeria, Spain; CIESOL Solar Energy Research Centre, 04120, Almeria, Spain
- 2HUN-REN SZTAKI, 13-17 Kende Street, 1111, Budapest, Hungary
- 3Department of Chemical Engineering, University of Almeria, 04120, Almeria, Spain; CIESOL Solar Energy Research Centre, 04120, Almeria, Spain; Corresponding author. Department of Chemical Engineering, University of Almeria, 04120, Almeria, Spain.
Abstract
This study evaluated the performance of HOLODETECT HiRes + FLUOR3, a laboratory instrument designed for detecting and counting small particles or cells in fluids using fluorescence. The test species were Haematococcus pluvialis and Chlorella sorokiniana because of their industrial importance in the functional food markets, and because they have been identified growing in co-culture, especially when the goal is to produce H . pluvialis . Initial trials focused on optimising the operating conditions to ensure accurate and reproducible measurements. The equipment demonstrated a low coefficient of variation (<5 %) for all the different determinations. Due to cell overlap, sample dilution was necessary in dense cultures prior to analysis. A second set of trials aimed to develop models (i) to identify the cell concentration in monocultures and co-cultures, and (ii) to determine the relative abundance of the two test species in mixed cultures. The models that were developed were able to provide cell count results comparable to those obtained using a Neubauer counting chamber. However, the model development step proved to be important, as different modelling approaches led to different results with some models overestimating and others underestimating the total cell count. Similarly, the system accurately quantifies the relative abundance of C . sorokiniana and H . pluvialis cells in cultures with different cell concentration ratios.
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BibTeX
@article{salinasgarcia2026highresolution,
title = {{High-resolution holographic reconstruction combined with fluorescence microscopy to identify unwanted}},
author = {María Salinas-García and Barbara Bicsák and Gabriel Acién and Tomás Lafarga},
journal = {Water Biology and Security},
year = {2026},
month = nov,
doi = {10.1016/j.watbs.2025.100546},
url = {https://doi.org/10.1016/j.watbs.2025.100546},
note = {CC BY},
}
RIS
TY - JOUR
TI - High-resolution holographic reconstruction combined with fluorescence microscopy to identify unwanted
AU - María Salinas-García
AU - Barbara Bicsák
AU - Gabriel Acién
AU - Tomás Lafarga
PY - 2026
DA - 2026/11/01
JO - Water Biology and Security
DO - 10.1016/j.watbs.2025.100546
UR - https://doi.org/10.1016/j.watbs.2025.100546
AB - This study evaluated the performance of HOLODETECT HiRes + FLUOR3, a laboratory instrument designed for detecting and counting small particles or cells in fluids using fluorescence. The test species were Haematococcus pluvialis and Chlorella sorokiniana because of their industrial importance in the functional food markets, and because they have been identified growing in co-culture, especially when the goal is to produce H . pluvialis . Initial trials focused on optimising the operating conditions to ensure accurate and reproducible measurements. The equipment demonstrated a low coefficient of variation (<5 %) for all the different determinations. Due to cell overlap, sample dilution was necessary in dense cultures prior to analysis. A second set of trials aimed to develop models (i) to identify the cell concentration in monocultures and co-cultures, and (ii) to determine the relative abundance of the two test species in mixed cultures. The models that were developed were able to provide cell count results comparable to those obtained using a Neubauer counting chamber. However, the model development step proved to be important, as different modelling approaches led to different results with some models overestimating and others underestimating the total cell count. Similarly, the system accurately quantifies the relative abundance of C . sorokiniana and H . pluvialis cells in cultures with different cell concentration ratios.
SN - 2772-7351
ER -