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Peer-reviewedCC BY 4.0Emerging Infectious Diseases

Differentiating Avian and Seasonal Influenza Viruses in Wastewater by Digital PCR

  • Valentina Paracchini
  • Mauro Petrillo
  • Francisco Mayo Leon
  • Gerhard Buttinger
  • Sabrina Marciano
  • Marika Crimaudo
  • Calogero Terregino
  • Carolina Veneri
  • Giuseppina La Rosa
  • Elisabetta Suffredini
  • Clementina Cocuzza
  • Bernd M. Gawlik
4 more authors
  • Tobias Wiesenthal
  • Ursula Vincent
  • Valentina Panzarin
  • Antonio Marchini
Read full textDOICite

Abstract

Highly pathogenic avian influenza A(H5Nx) viruses, particularly clade 2.3.4.4b, pose substantial global health risks. We combined and piloted 2 duplex reverse transcription digital PCRs to discriminate between avian and seasonal influenza viruses in wastewater. This approach enabled rapid, strain-specific detection to reduce ambiguity in environmental surveillance for influenza viruses.

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Cite

BibTeXRIS
BibTeX
@article{paracchini2026differentiating,
  title = {{Differentiating Avian and Seasonal Influenza Viruses in Wastewater by Digital PCR}},
  author = {Valentina Paracchini and Mauro Petrillo and Francisco Mayo Leon and Gerhard Buttinger and Sabrina Marciano and Marika Crimaudo and Calogero Terregino and Carolina Veneri and Giuseppina La Rosa and Elisabetta Suffredini and Clementina Cocuzza and Bernd M. Gawlik and Tobias Wiesenthal and Ursula Vincent and Valentina Panzarin and Antonio Marchini},
  journal = {Emerging Infectious Diseases},
  year = {2026},
  month = oct,
  doi = {10.3201/eid3211.261234},
  url = {https://doi.org/10.3201/eid3211.261234},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Differentiating Avian and Seasonal Influenza Viruses in Wastewater by Digital PCR
AU  - Valentina Paracchini
AU  - Mauro Petrillo
AU  - Francisco Mayo Leon
AU  - Gerhard Buttinger
AU  - Sabrina Marciano
AU  - Marika Crimaudo
AU  - Calogero Terregino
AU  - Carolina Veneri
AU  - Giuseppina La Rosa
AU  - Elisabetta Suffredini
AU  - Clementina Cocuzza
AU  - Bernd M. Gawlik
AU  - Tobias Wiesenthal
AU  - Ursula Vincent
AU  - Valentina Panzarin
AU  - Antonio Marchini
PY  - 2026
DA  - 2026/10/30
JO  - Emerging Infectious Diseases
DO  - 10.3201/eid3211.261234
UR  - https://doi.org/10.3201/eid3211.261234
AB  - Highly pathogenic avian influenza A(H5Nx) viruses, particularly clade 2.3.4.4b, pose substantial global health risks. We combined and piloted 2 duplex reverse transcription digital PCRs to discriminate between avian and seasonal influenza viruses in wastewater. This approach enabled rapid, strain-specific detection to reduce ambiguity in environmental surveillance for influenza viruses.
SN  - 1080-6040
SN  - 1080-6059
ER  -