Modeling Gene Expression Noise: From Transcriptional Dynamics to Cell Growth and Division
Abstract
Gene expression is inherently noisy, leading to substantial cell-to-cell variation in mRNA and protein levels. The telegraph model and the simpler bursty model provide good fits to single-cell count distributions and have been used extensively to understand transcriptional kinetics and estimate the kinetic parameters. However, these models all describe a static cell. Here, we review models of stochastic gene expression in growing and dividing cells which aim to understand how a cell's own life history, from its birth, through copying its genome, to its division, leaves a distinct fluctuation signature on molecule numbers. We discuss the biochemical and biophysical processes captured by these models, their analytical tractability, the concept of cyclo-stationary behavior, when this is well approximated by the steady-state of static models and how the distributions of molecular numbers measured under these conditions sensitively depend on the experimental setup. This is followed by a discussion of how these models are fitted to single-cell measurements, how biological variability can be separated from measurement noise, and which computational advances make this feasible for thousands of genes. We finish by highlighting the open problems in the field.
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BibTeX
@misc{sukys2026modeling,
title = {{Modeling Gene Expression Noise: From Transcriptional Dynamics to Cell Growth and Division}},
author = {Augustinas Sukys and Lucy Ham and Kaan Öcal and James Holehouse and Ramon Grima},
howpublished = {arXiv},
year = {2026},
month = nov,
doi = {10.48550/arxiv.2611.06537},
eprint = {2611.06537},
archivePrefix = {arXiv},
url = {https://arxiv.org/abs/2611.06537},
note = {CC BY 4.0},
}
RIS
TY - UNPB
TI - Modeling Gene Expression Noise: From Transcriptional Dynamics to Cell Growth and Division
AU - Augustinas Sukys
AU - Lucy Ham
AU - Kaan Öcal
AU - James Holehouse
AU - Ramon Grima
PY - 2026
DA - 2026/11/04
PB - arXiv
DO - 10.48550/arxiv.2611.06537
UR - https://arxiv.org/abs/2611.06537
AB - Gene expression is inherently noisy, leading to substantial cell-to-cell variation in mRNA and protein levels. The telegraph model and the simpler bursty model provide good fits to single-cell count distributions and have been used extensively to understand transcriptional kinetics and estimate the kinetic parameters. However, these models all describe a static cell. Here, we review models of stochastic gene expression in growing and dividing cells which aim to understand how a cell's own life history, from its birth, through copying its genome, to its division, leaves a distinct fluctuation signature on molecule numbers. We discuss the biochemical and biophysical processes captured by these models, their analytical tractability, the concept of cyclo-stationary behavior, when this is well approximated by the steady-state of static models and how the distributions of molecular numbers measured under these conditions sensitively depend on the experimental setup. This is followed by a discussion of how these models are fitted to single-cell measurements, how biological variability can be separated from measurement noise, and which computational advances make this feasible for thousands of genes. We finish by highlighting the open problems in the field.
ER -