Integrated genomic and transcriptomic analysis reveals the biocontrol and plant growth-promoting mechanisms of Burkholderia gladioli PNB20-1 against peanut southern blight
Abstract
IntroductionPeanut southern blight, caused by Sclerotium rolfsii, is a devastating soilborne disease that threatens peanut production worldwide. Members of Burkholderia have attracted attention as biocontrol agents owing to their diverse secondary metabolites and plant growth-promoting (PGP) capacity.MethodsWe isolated B. gladioli PNB20-1 from the disease-healthy junction of peanut plants. Its biocontrol and PGP potential was evaluated by dual-culture, greenhouse pot, and biosafety assays, its genome was sequenced on the Oxford Nanopore platform, and host defense responses were profiled by RNA-Seq.ResultsPNB20-1 exhibited in vitro antagonism against S. rolfsii with a 52.7% inhibition rate and reduced sclerotial biomass by 19.3% after 4 weeks of co-culture. The complete genome of PNB20-1 comprised 8,278,972 bp across three circular replicons, encoding 6,923 CDSs and 21 biosynthetic gene clusters (BGCs). Among these, the ornibactin-type siderophore BGC and phosphate solubilization genes were supported by phenotypic assays. Transcriptomic profiling of PNB20-1-inoculated peanut roots identified 1,552 differentially expressed genes, of which 1,057 were upregulated and 495 downregulated. GO and KEGG enrichment analyses pointed to a coordinated defense response encompassing stilbenoid phytoalexin biosynthesis, cutin-, suberin-, and lignin-mediated apoplastic barrier reinforcement, and salicylic acid (SA)-dominated multi-hormonal signaling. Preliminary biosafety evaluation showed that PNB20-1 is non-hemolytic and lacks key virulence determinants of clinically significant Burkholderia species.DiscussionThese findings suggest that B. gladioli PNB20-1 is a promising biocontrol agent and provide insights into the molecular basis of Burkholderia-mediated disease suppression in peanut.
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BibTeX
@article{xu2026integrated,
title = {{Integrated genomic and transcriptomic analysis reveals the biocontrol and plant growth-promoting mechanisms of Burkholderia gladioli PNB20-1 against peanut southern blight}},
author = {Jinyan Xu and Yonghui Liu and Man Liang and Weiqi Chen and Yi Shen},
journal = {Frontiers in Microbiology},
year = {2026},
month = nov,
doi = {10.3389/fmicb.2026.1984673},
url = {https://www.frontiersin.org/articles/10.3389/fmicb.2026.1984673},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Integrated genomic and transcriptomic analysis reveals the biocontrol and plant growth-promoting mechanisms of Burkholderia gladioli PNB20-1 against peanut southern blight
AU - Jinyan Xu
AU - Yonghui Liu
AU - Man Liang
AU - Weiqi Chen
AU - Yi Shen
PY - 2026
DA - 2026/11/04
JO - Frontiers in Microbiology
DO - 10.3389/fmicb.2026.1984673
UR - https://www.frontiersin.org/articles/10.3389/fmicb.2026.1984673
AB - IntroductionPeanut southern blight, caused by Sclerotium rolfsii, is a devastating soilborne disease that threatens peanut production worldwide. Members of Burkholderia have attracted attention as biocontrol agents owing to their diverse secondary metabolites and plant growth-promoting (PGP) capacity.MethodsWe isolated B. gladioli PNB20-1 from the disease-healthy junction of peanut plants. Its biocontrol and PGP potential was evaluated by dual-culture, greenhouse pot, and biosafety assays, its genome was sequenced on the Oxford Nanopore platform, and host defense responses were profiled by RNA-Seq.ResultsPNB20-1 exhibited in vitro antagonism against S. rolfsii with a 52.7% inhibition rate and reduced sclerotial biomass by 19.3% after 4 weeks of co-culture. The complete genome of PNB20-1 comprised 8,278,972 bp across three circular replicons, encoding 6,923 CDSs and 21 biosynthetic gene clusters (BGCs). Among these, the ornibactin-type siderophore BGC and phosphate solubilization genes were supported by phenotypic assays. Transcriptomic profiling of PNB20-1-inoculated peanut roots identified 1,552 differentially expressed genes, of which 1,057 were upregulated and 495 downregulated. GO and KEGG enrichment analyses pointed to a coordinated defense response encompassing stilbenoid phytoalexin biosynthesis, cutin-, suberin-, and lignin-mediated apoplastic barrier reinforcement, and salicylic acid (SA)-dominated multi-hormonal signaling. Preliminary biosafety evaluation showed that PNB20-1 is non-hemolytic and lacks key virulence determinants of clinically significant Burkholderia species.DiscussionThese findings suggest that B. gladioli PNB20-1 is a promising biocontrol agent and provide insights into the molecular basis of Burkholderia-mediated disease suppression in peanut.
SN - 1664-302X
ER -