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Peer-reviewedCC BY 4.0Cells

Selective Estradiol Sensitivity in 12Z Human Endometriotic Epithelial Cell Line

  • Shohini Banerjee1
  • Esha Chopra1
  • Ian M. Smith1
  • Kimberly M. Stroka1,2,3,4
4 affiliations
  1. 1Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA
  2. 2Marlene and Stewart Greenebaum Comprehensive Cancer Center, University of Maryland, Baltimore, MD 21201, USA
  3. 3Biophysics Program, University of Maryland, College Park, MD 20742, USA
  4. 4Center for Stem Cell Biology and Regenerative Medicine, University of Maryland, Baltimore, MD 21201, USA
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Abstract

Estradiol (E2) is a potent estrogen molecule that plays a crucial role in regulating numerous healthy and pathophysiological processes. To model estrogen-dependent cellular activities in vitro in a physiologically meaningful way, cells chosen for experimentation should exhibit sensitivity to E2. The 12Z human endometriotic epithelial cell line is gaining attention as a model for estrogen-dependent conditions, but its functional responsiveness to E2 has not been well characterized. For the passage range in the following study, we found that E2 does not statistically significantly influence 12Z proliferation, wound closure, or single-cell migration. However, 12Zs were selectively responsive to E2 in 3D migration models where the cells were physically confined. Upregulating ESR1, the primary mediator of estrogenic action, in the 12Zs did not enhance their functional E2 sensitivity. These results highlight the importance of verifying hormone responsiveness when using 12Z cells to model estrogen-dependent processes and conditions.

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Cite

BibTeXRIS
BibTeX
@article{banerjee2026selective,
  title = {{Selective Estradiol Sensitivity in 12Z Human Endometriotic Epithelial Cell Line}},
  author = {Shohini Banerjee and Esha Chopra and Ian M. Smith and Kimberly M. Stroka},
  journal = {Cells},
  year = {2026},
  month = oct,
  doi = {10.3390/cells15201875},
  url = {https://www.mdpi.com/2073-4409/15/20/1875},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Selective Estradiol Sensitivity in 12Z Human Endometriotic Epithelial Cell Line
AU  - Shohini Banerjee
AU  - Esha Chopra
AU  - Ian M. Smith
AU  - Kimberly M. Stroka
PY  - 2026
DA  - 2026/10/15
JO  - Cells
DO  - 10.3390/cells15201875
UR  - https://www.mdpi.com/2073-4409/15/20/1875
AB  - Estradiol (E2) is a potent estrogen molecule that plays a crucial role in regulating numerous healthy and pathophysiological processes. To model estrogen-dependent cellular activities in vitro in a physiologically meaningful way, cells chosen for experimentation should exhibit sensitivity to E2. The 12Z human endometriotic epithelial cell line is gaining attention as a model for estrogen-dependent conditions, but its functional responsiveness to E2 has not been well characterized. For the passage range in the following study, we found that E2 does not statistically significantly influence 12Z proliferation, wound closure, or single-cell migration. However, 12Zs were selectively responsive to E2 in 3D migration models where the cells were physically confined. Upregulating ESR1, the primary mediator of estrogenic action, in the 12Zs did not enhance their functional E2 sensitivity. These results highlight the importance of verifying hormone responsiveness when using 12Z cells to model estrogen-dependent processes and conditions.
SN  - 2073-4409
ER  -