Development of a peptide-based bioassay for zearalenone detection via phage display and in silico analysis
- 1School of Life Science and Technology, Changchun University of Science and Technology, Changchun, China
- 2Lingkang Times Biotechnology (Changchun) Co., Ltd, Changchun City, Jilin, China
Abstract
Zearalenone (ZEN) is a prevalent mycotoxin threatening food and feed safety, necessitating rapid and reliable detection methods. A ZEN-binding peptide was isolated from a 12-mer phage display library and characterized by molecular docking, synthesis, HPLC purification, and streptavidin-HRP-based ELISA. Specificity, storage stability, and actual grain sample recovery rates were evaluated. The predicted binding free energy between the peptide G-W-H-V-H-S-H-R-F-A-S-I and ZEN was −4.95 kcal/mol. The biotinylated probe reached 98.77% purity and detected ZEN with a limit of detection of approximately 5 ng/mL. The cross-reactivity with other mycotoxins and ZEN analogues was below 10%. The detection responses were highly consistent between mixed and single-ZEN mycotoxin systems. The probe remained stable at 4 and 25 °C for 28 days. With recoveries of 87.43%–108.10% in spiked grains and 80.0%–89.5% in reference materials. This peptide provides a stable, synthesis-friendly recognition element for ZEN detection and future bioassay applications.
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BibTeX
@article{wang2026development,
title = {{Development of a peptide-based bioassay for zearalenone detection via phage display and in silico analysis}},
author = {Siya Wang and Yulin Sui and Yan Pang and Shuhua Zhang},
journal = {Food and Agricultural Immunology},
year = {2026},
month = oct,
doi = {10.1080/09540105.2026.2749211},
url = {https://doi.org/10.1080/09540105.2026.2749211},
note = {CC BY},
}
RIS
TY - JOUR
TI - Development of a peptide-based bioassay for zearalenone detection via phage display and in silico analysis
AU - Siya Wang
AU - Yulin Sui
AU - Yan Pang
AU - Shuhua Zhang
PY - 2026
DA - 2026/10/30
JO - Food and Agricultural Immunology
DO - 10.1080/09540105.2026.2749211
UR - https://doi.org/10.1080/09540105.2026.2749211
AB - Zearalenone (ZEN) is a prevalent mycotoxin threatening food and feed safety, necessitating rapid and reliable detection methods. A ZEN-binding peptide was isolated from a 12-mer phage display library and characterized by molecular docking, synthesis, HPLC purification, and streptavidin-HRP-based ELISA. Specificity, storage stability, and actual grain sample recovery rates were evaluated. The predicted binding free energy between the peptide G-W-H-V-H-S-H-R-F-A-S-I and ZEN was −4.95 kcal/mol. The biotinylated probe reached 98.77% purity and detected ZEN with a limit of detection of approximately 5 ng/mL. The cross-reactivity with other mycotoxins and ZEN analogues was below 10%. The detection responses were highly consistent between mixed and single-ZEN mycotoxin systems. The probe remained stable at 4 and 25 °C for 28 days. With recoveries of 87.43%–108.10% in spiked grains and 80.0%–89.5% in reference materials. This peptide provides a stable, synthesis-friendly recognition element for ZEN detection and future bioassay applications.
SN - 1465-3443
SN - 0954-0105
ER -