Modulation of Osteoprotegerin Expression by Chicken Feet Collagen–Chitosan Combination in Wistar Rats: An In vivo Experimental Study
Abstract
Background: Bone regeneration is a regulated process involving osteoblast and osteoclast activity. Osteoprotegerin (OPG) plays a key role by inhibiting osteoclastogenesis and maintaining the balance between bone formation and resorption. Natural biomaterials such as collagen and chitosan are widely studied for their biocompatibility and bioactivity; however, evidence on the combined use of chicken feet-derived collagen and shrimp shell chitosan remains limited. Aim: This study aimed to evaluate the effects of chicken feet collagen, shrimp shell chitosan, and their combination on OPG expression in the mandibular bone of Wistar rats. Subjects and Methods: A posttest-only in vivo experimental design was conducted using 24 male Wistar rats randomly assigned to four groups: control, collagen, chitosan, and collagen–chitosan combination, with observation periods of 21 and 28 days postsurgery. Treatments were administered orally once daily. Mandibular bone samples were collected, and OPG expression was analyzed using immunohistochemistry. Statistical analysis was performed using the Kruskal–Wallis test followed by Mann–Whitney U test post hoc tests. Results: The results showed a significant difference in OPG expression among groups (P = 0.004). The combination group demonstrated the highest OPG expression, significantly higher than the control group (P < 0.01). No significant difference was observed between the 21- and 28-day combination groups (P > 0.05). Conclusion: The combination of chicken feet collagen and shrimp shell chitosan synergistically enhances OPG expression, indicating its potential as a sustainable biomaterial for bone regeneration
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BibTeX
@article{azzahra2026modulation,
title = {{Modulation of Osteoprotegerin Expression by Chicken Feet Collagen–Chitosan Combination in Wistar Rats: An In vivo Experimental Study}},
author = {Khusnul Fatimah Azzahra and Sherly Horax and Lusy Damayanti and Yayah Inayah},
journal = {Contemporary Clinical Dentistry},
year = {2026},
month = jul,
doi = {10.4103/ccd.ccd_47_26},
url = {https://doi.org/10.4103/ccd.ccd_47_26},
note = {CC BY-NC-SA},
}
RIS
TY - JOUR
TI - Modulation of Osteoprotegerin Expression by Chicken Feet Collagen–Chitosan Combination in Wistar Rats: An In vivo Experimental Study
AU - Khusnul Fatimah Azzahra
AU - Sherly Horax
AU - Lusy Damayanti
AU - Yayah Inayah
PY - 2026
DA - 2026/07/01
JO - Contemporary Clinical Dentistry
DO - 10.4103/ccd.ccd_47_26
UR - https://doi.org/10.4103/ccd.ccd_47_26
AB - Background: Bone regeneration is a regulated process involving osteoblast and osteoclast activity. Osteoprotegerin (OPG) plays a key role by inhibiting osteoclastogenesis and maintaining the balance between bone formation and resorption. Natural biomaterials such as collagen and chitosan are widely studied for their biocompatibility and bioactivity; however, evidence on the combined use of chicken feet-derived collagen and shrimp shell chitosan remains limited. Aim: This study aimed to evaluate the effects of chicken feet collagen, shrimp shell chitosan, and their combination on OPG expression in the mandibular bone of Wistar rats. Subjects and Methods: A posttest-only in vivo experimental design was conducted using 24 male Wistar rats randomly assigned to four groups: control, collagen, chitosan, and collagen–chitosan combination, with observation periods of 21 and 28 days postsurgery. Treatments were administered orally once daily. Mandibular bone samples were collected, and OPG expression was analyzed using immunohistochemistry. Statistical analysis was performed using the Kruskal–Wallis test followed by Mann–Whitney U test post hoc tests. Results: The results showed a significant difference in OPG expression among groups (P = 0.004). The combination group demonstrated the highest OPG expression, significantly higher than the control group (P < 0.01). No significant difference was observed between the 21- and 28-day combination groups (P > 0.05). Conclusion: The combination of chicken feet collagen and shrimp shell chitosan synergistically enhances OPG expression, indicating its potential as a sustainable biomaterial for bone regeneration
SN - 0976-237X
SN - 0976-2361
ER -