Interfacial Assembly of Marine Exopolysaccharide EPS MO245 Reduces Pseudomonas aeruginosa Adhesion on Silica
Abstract
Marine exopolysaccharides are increasingly explored as non-biocidal anti-adhesive agents, yet how their assembly at the solid-liquid interface, rather than their intrinsic chemistry alone, governs this performance remains poorly understood. Here we compare the adsorption of the marine exopolysaccharide EPS MO245 and hyaluronic acid (HA) on silica and examine how the resulting interfacial layers control the adhesion of Pseudomonas aeruginosa PAO1. Quartz crystal microbalance with dissipation (QCM-D) measurements show that EPS MO245 adsorbs onto bare silica under specific physicochemical conditions, promoted at low pH and high ionic strength, whereas HA shows no measurable adsorption under the same conditions. Although both polysaccharides are negatively charged, these contrasting behaviors demonstrate that electrostatic interactions alone cannot account for their interfacial assembly. Purification and ion-complexation experiments indicate that natively associated species, including divalent cations, contribute to EPS MO245 adsorption but do not fully
Related
- Membrane damage induces TagQ/TagR complex-mediated H1-T6SS assembly in Pseudomonas aeruginosa
- Host immune stress reveals a role for potassium homeostasis in Pseudomonas aeruginosa aggregate stability
- LytM family endopeptidase LmdC is controlled by regulated auto processing and proteolytic destruction in Pseudomonas aeruginosa
- Mining the Pseudomonas aeruginosa genome for regulators of type III secretion system gene expression using FT-Tn-seq
- Auranofin shows bactericidal activity in Pseudomonas aeruginosa by targeting thiol homeostasis
BibTeX
@article{champion2026interfacial,
title = {{Interfacial Assembly of Marine Exopolysaccharide EPS MO245 Reduces Pseudomonas aeruginosa Adhesion on Silica}},
author = {Marie Champion and Jean-Paul Chapel and Xavier Moppert and Eric Balnois and Fabienne Faÿ and Guillaume Vignaud},
journal = {Colloids and Surfaces A: Physicochemical and Engineering Aspects},
year = {2026},
month = sep,
doi = {10.1016/j.colsurfa.2026.141804},
url = {https://hal.science/hal-05755145v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Interfacial Assembly of Marine Exopolysaccharide EPS MO245 Reduces Pseudomonas aeruginosa Adhesion on Silica
AU - Marie Champion
AU - Jean-Paul Chapel
AU - Xavier Moppert
AU - Eric Balnois
AU - Fabienne Faÿ
AU - Guillaume Vignaud
PY - 2026
DA - 2026/09/18
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
DO - 10.1016/j.colsurfa.2026.141804
UR - https://hal.science/hal-05755145v1
AB - Marine exopolysaccharides are increasingly explored as non-biocidal anti-adhesive agents, yet how their assembly at the solid-liquid interface, rather than their intrinsic chemistry alone, governs this performance remains poorly understood. Here we compare the adsorption of the marine exopolysaccharide EPS MO245 and hyaluronic acid (HA) on silica and examine how the resulting interfacial layers control the adhesion of Pseudomonas aeruginosa PAO1. Quartz crystal microbalance with dissipation (QCM-D) measurements show that EPS MO245 adsorbs onto bare silica under specific physicochemical conditions, promoted at low pH and high ionic strength, whereas HA shows no measurable adsorption under the same conditions. Although both polysaccharides are negatively charged, these contrasting behaviors demonstrate that electrostatic interactions alone cannot account for their interfacial assembly. Purification and ion-complexation experiments indicate that natively associated species, including divalent cations, contribute to EPS MO245 adsorption but do not fully
SN - 0927-7757
SN - 1873-4359
ER -