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Peer-reviewedCC BYJournal of proteome research

Toward Optimized Proteomics Analysis to Detect Bacterial and Host Proteins in Calcified Coronary Artery Plaques.

  • Kirsikka Musta1
  • Markus J T Ojanen1
  • Maria Stensland2
  • Jonna Kankaanranta1
  • Terho Lehtimäki1,3,4
  • Pekka J Karhunen1,5,4
  • Tuula A Nyman2
  • Vesa P Hytönen1,5
5 affiliations
  1. 1Faculty of Medicine and Health Technology, Tampere University, Tampere33520, Finland.
  2. 2Department of Immunology, University of Oslo and Oslo University Hospital, Oslo0372, Norway.
  3. 3Department of Clinical Chemistry, Fimlab laboratoriesTampere33520, Finland.
  4. 4Finnish Cardiovascular Research Center - Tampere, Faculty of Medicine and Health Technology, Tampere University, Tampere33520, Finland.
  5. 5Fimlab Laboratories, Tampere33520, Finland.
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Abstract

Coronary calcification is the final stage in coronary heart disease, associated with significantly increased morbidity and mortality. The molecular pathology of calcification and especially events triggering calcification are poorly understood. Metagenomic and molecular microbial techniques have identified the presence of oral biofilm-producing and dental calculus-forming bacteria in atherosclerotic plaques, suggesting that they may be involved in calcification. To enable studying this at the proteome level, we compared four different protein extraction methods to find a robust and reliable method for the identification and quantification of human and bacterial proteins from 10 calcified arterial plaques. We identified 3 913 human proteins and 24 bacterial proteins. These findings provide further support for the presence of bacterial proteins in calcified areas of coronary artery plaques, with Fusobacteria being the most abundant bacterial protein source. Comparisons of the extraction methods showed that the urea-based extraction method was most optimal due to its simplicity and reproducible identification of a high number of proteins, which makes it suitable for further large-scale analysis of coronary artery plaque samples.

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Cite

BibTeXRIS
BibTeX
@article{musta2026toward,
  title = {{Toward Optimized Proteomics Analysis to Detect Bacterial and Host Proteins in Calcified Coronary Artery Plaques.}},
  author = {Kirsikka Musta and Markus J T Ojanen and Maria Stensland and Jonna Kankaanranta and Terho Lehtimäki and Pekka J Karhunen and Tuula A Nyman and Vesa P Hytönen},
  journal = {Journal of proteome research},
  year = {2026},
  month = oct,
  doi = {10.1021/acs.jproteome.6c00422},
  url = {https://europepmc.org/article/MED/42918121},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Toward Optimized Proteomics Analysis to Detect Bacterial and Host Proteins in Calcified Coronary Artery Plaques.
AU  - Kirsikka Musta
AU  - Markus J T Ojanen
AU  - Maria Stensland
AU  - Jonna Kankaanranta
AU  - Terho Lehtimäki
AU  - Pekka J Karhunen
AU  - Tuula A Nyman
AU  - Vesa P Hytönen
PY  - 2026
DA  - 2026/10/01
JO  - Journal of proteome research
DO  - 10.1021/acs.jproteome.6c00422
UR  - https://europepmc.org/article/MED/42918121
AB  - Coronary calcification is the final stage in coronary heart disease, associated with significantly increased morbidity and mortality. The molecular pathology of calcification and especially events triggering calcification are poorly understood. Metagenomic and molecular microbial techniques have identified the presence of oral biofilm-producing and dental calculus-forming bacteria in atherosclerotic plaques, suggesting that they may be involved in calcification. To enable studying this at the proteome level, we compared four different protein extraction methods to find a robust and reliable method for the identification and quantification of human and bacterial proteins from 10 calcified arterial plaques. We identified 3 913 human proteins and 24 bacterial proteins. These findings provide further support for the presence of bacterial proteins in calcified areas of coronary artery plaques, with Fusobacteria being the most abundant bacterial protein source. Comparisons of the extraction methods showed that the urea-based extraction method was most optimal due to its simplicity and reproducible identification of a high number of proteins, which makes it suitable for further large-scale analysis of coronary artery plaque samples.
SN  - 1535-3893
SN  - 1535-3907
ER  -