CD177 gene polymorphisms governing the differential expression of HNA-2 on neutrophils in Indians
Abstract
BackgroundCD177gp, also known as human neutrophil antigen (HNA)-2, is encoded by the CD177 gene located on chromosome 19. Different molecular mechanisms involving polymorphisms in the gene, mRNA splicing defects, DNA methylation, and so on govern the expression of HNA-2 on neutrophils ranging from 0% to 100%, with the unique biphenotypic expression observed in the majority of individuals. Its genotyping is often complicated by the presence of the pseudogene, CD177P1.MethodsIn the present study, the CD177gp expression on the neutrophils of 102 individuals from Mumbai, India, was compared via flow cytometry using two clones, namely MEM-166 and TAG4. For genotyping of the CD177 gene, long-range PCR was employed. Phenotypic expression was then correlated with the CD177 gene polymorphisms to determine the molecular basis of HNA-2 in Indians.ResultsOverall, the median CD177 expression using the MEM-166 clone was 60.4% (Q1–Q3: 48.5–70.8), and using the TAG4 clone, it was 58.4% (Q1–Q3: 45.9–72.9). A statistically significant difference of 1.56% clone-dependent variation in expression was observed between the two clones (p = 0.007). On molecular genotyping, 17 different genotype patterns and 8 novel mutations were identified. The multi-locus genotype pattern containing NM_020406.4:c.787A>T was found in a heterozygous state in 8.8% of the samples, of which 66.67% of the samples had low expression of CD177gp.ConclusionThis is the first study reporting the molecular characterization of the CD177 gene in individuals from Mumbai, India.
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BibTeX
@article{maru2026cd177,
title = {{CD177 gene polymorphisms governing the differential expression of HNA-2 on neutrophils in Indians}},
author = {Harita Maru and Amrutha Jose and Manisha Madkaikar},
journal = {Frontiers in Immunology},
year = {2026},
month = nov,
doi = {10.3389/fimmu.2026.1961339},
url = {https://www.frontiersin.org/articles/10.3389/fimmu.2026.1961339},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - CD177 gene polymorphisms governing the differential expression of HNA-2 on neutrophils in Indians
AU - Harita Maru
AU - Amrutha Jose
AU - Manisha Madkaikar
PY - 2026
DA - 2026/11/06
JO - Frontiers in Immunology
DO - 10.3389/fimmu.2026.1961339
UR - https://www.frontiersin.org/articles/10.3389/fimmu.2026.1961339
AB - BackgroundCD177gp, also known as human neutrophil antigen (HNA)-2, is encoded by the CD177 gene located on chromosome 19. Different molecular mechanisms involving polymorphisms in the gene, mRNA splicing defects, DNA methylation, and so on govern the expression of HNA-2 on neutrophils ranging from 0% to 100%, with the unique biphenotypic expression observed in the majority of individuals. Its genotyping is often complicated by the presence of the pseudogene, CD177P1.MethodsIn the present study, the CD177gp expression on the neutrophils of 102 individuals from Mumbai, India, was compared via flow cytometry using two clones, namely MEM-166 and TAG4. For genotyping of the CD177 gene, long-range PCR was employed. Phenotypic expression was then correlated with the CD177 gene polymorphisms to determine the molecular basis of HNA-2 in Indians.ResultsOverall, the median CD177 expression using the MEM-166 clone was 60.4% (Q1–Q3: 48.5–70.8), and using the TAG4 clone, it was 58.4% (Q1–Q3: 45.9–72.9). A statistically significant difference of 1.56% clone-dependent variation in expression was observed between the two clones (p = 0.007). On molecular genotyping, 17 different genotype patterns and 8 novel mutations were identified. The multi-locus genotype pattern containing NM_020406.4:c.787A>T was found in a heterozygous state in 8.8% of the samples, of which 66.67% of the samples had low expression of CD177gp.ConclusionThis is the first study reporting the molecular characterization of the CD177 gene in individuals from Mumbai, India.
SN - 1664-3224
ER -