Fridericia chica standardized extract combined with tocotrienol-enriched fraction from Bixa orellana promotes gastric mucosal protection: in vitro combination selection and in vivo evaluation.
- 1School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil.
Abstract
Context
Fridericia chica (Bonpl.) L.G. Lohmann is a medicinal plant rich in bioactive 3-deoxyanthocyanidins with reported wound-healing and gastroprotective properties. A 70% tocotrienol-enriched fraction (TRF) from Bixa orellana L. exhibits antioxidant and anti-inflammatory activities that may complement those of F. chica in gastric mucosal protection.Objective
This study aimed to evaluate the gastroprotective and gastric tissue-repair effects of the F. chica-TRF combination.Materials and methods
Dry F. chica standardized crude extract alone or combined with TRF at different proportions was tested in vitro in HaCaT keratinocytes and 3T3 fibroblasts to assess cell viability and select a suitable combination ratio for subsequent in vivo investigation. The selected ratio was then investigated in acute and chronic gastric ulcer models in female Wistar rats.Results
Among the proportions tested in vitro, the 90:10 (F. chica:TRF) ratio showed the most favorable cell-viability profile and was selected for in vivo evaluation. The combination reduced gastric lesions and significantly increased gastric mucus production. In the chronic ulcer model, treatment was associated with reduced TNF-α and IL-1β levels and increased IL-10 levels. Histopathological and second-harmonic generation analyses qualitatively indicated mucosal preservation, tissue organization, and collagen deposition.Conclusions
The F. chica-TRF combination exhibited gastroprotective effects associated with increased mucus production and modulation of inflammatory mediators. Qualitative morphological findings further indicated preservation of gastric tissue architecture and collagen organization, supporting the potential of this combination as a natural product-based approach to gastric mucosal protection.
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BibTeX
@article{freitas2026fridericia,
title = {{Fridericia chica standardized extract combined with tocotrienol-enriched fraction from Bixa orellana promotes gastric mucosal protection: in vitro combination selection and in vivo evaluation.}},
author = {Ailane Souza de Freitas and Ilza Maria de Oliveira Sousa and Fabricio de Faveri Favero and Josélia Cristina de Oliveira and Robson da Silva Pontes and Alex Vinicius de Campos and Clara Giuliana Magossi and Mayra Gabriella Biccigo and Ana Lucia Tasca Gois Ruiz and João Ernesto de Carvalho and Mary Ann Foglio},
journal = {Pharmaceutical biology},
year = {2026},
month = oct,
doi = {10.1080/13880209.2026.2751273},
url = {https://europepmc.org/article/MED/42921817},
note = {CC BY},
}
RIS
TY - JOUR
TI - Fridericia chica standardized extract combined with tocotrienol-enriched fraction from Bixa orellana promotes gastric mucosal protection: in vitro combination selection and in vivo evaluation.
AU - Ailane Souza de Freitas
AU - Ilza Maria de Oliveira Sousa
AU - Fabricio de Faveri Favero
AU - Josélia Cristina de Oliveira
AU - Robson da Silva Pontes
AU - Alex Vinicius de Campos
AU - Clara Giuliana Magossi
AU - Mayra Gabriella Biccigo
AU - Ana Lucia Tasca Gois Ruiz
AU - João Ernesto de Carvalho
AU - Mary Ann Foglio
PY - 2026
DA - 2026/10/24
JO - Pharmaceutical biology
DO - 10.1080/13880209.2026.2751273
UR - https://europepmc.org/article/MED/42921817
AB - Context Fridericia chica (Bonpl.) L.G. Lohmann is a medicinal plant rich in bioactive 3-deoxyanthocyanidins with reported wound-healing and gastroprotective properties. A 70% tocotrienol-enriched fraction (TRF) from Bixa orellana L. exhibits antioxidant and anti-inflammatory activities that may complement those of F. chica in gastric mucosal protection.Objective This study aimed to evaluate the gastroprotective and gastric tissue-repair effects of the F. chica-TRF combination.Materials and methods Dry F. chica standardized crude extract alone or combined with TRF at different proportions was tested in vitro in HaCaT keratinocytes and 3T3 fibroblasts to assess cell viability and select a suitable combination ratio for subsequent in vivo investigation. The selected ratio was then investigated in acute and chronic gastric ulcer models in female Wistar rats.Results Among the proportions tested in vitro, the 90:10 (F. chica:TRF) ratio showed the most favorable cell-viability profile and was selected for in vivo evaluation. The combination reduced gastric lesions and significantly increased gastric mucus production. In the chronic ulcer model, treatment was associated with reduced TNF-α and IL-1β levels and increased IL-10 levels. Histopathological and second-harmonic generation analyses qualitatively indicated mucosal preservation, tissue organization, and collagen deposition.Conclusions The F. chica-TRF combination exhibited gastroprotective effects associated with increased mucus production and modulation of inflammatory mediators. Qualitative morphological findings further indicated preservation of gastric tissue architecture and collagen organization, supporting the potential of this combination as a natural product-based approach to gastric mucosal protection.
SN - 1388-0209
SN - 1744-5116
ER -