Pantethine Restores Epidermal Homeostasis and Modulates Vesicle-Associated Proteins in Psoriasis.
7 affiliations
- 1Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
- 2Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
- 3Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences and Center of Excellence in Bioactive Resources for Innovative Clinical Applications, Chulalongkorn University, Bangkok, Thailand.
- 4The Academy of Science, The Royal Society of Thailand, Bangkok, Thailand.
- 5Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand.
- 6Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
- 7Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Abstract
Psoriasis is a chronic inflammatory skin disease characterized by disrupted epidermal homeostasis driven by interconnected immuno-inflammatory, metabolic, and redox signalling networks. Vesicle-associated intercellular communication has emerged as an important contributor to inflammatory amplification and tissue remodelling within the epidermal microenvironment. Pantethine, a stable vitamin B5 derivative with lipid-modulatory and anti-inflammatory properties, has been implicated in regulation of cellular stress responses and membrane-associated signalling pathways, suggesting potential therapeutic relevance in psoriasis. Here, we investigated the effects of pantethine using a full-thickness 3D human psoriatic skin model following keratinocyte safety assessment. Integrated histopathological, immunohistochemical, quantitative RT-PCR, and proteomic analyses were employed to interrogate inflammatory, oxidative, proliferative, apoptotic, differentiation-associated and vesicle-related responses. Pantethine significantly reduced epidermal thickening and normalized multiple psoriasis-associated molecular signatures. Proteomic profiling revealed coordinated modulation of vesicle-associated, cytoskeletal and extracellular matrix-related proteins, accompanied by reduced expression of exosome-associated markers CD63 and CD9. Pantethine suppressed pro-inflammatory signalling involving IL-1β, TNF-α, IL-17, S100 family members, and hyperproliferative keratin-associated pathways while reducing caspase-3-associated apoptotic activity. Concurrently, pantethine enhanced antioxidative and immunoregulatory responses through activation of Nrf-2 and TGF-β, restored differentiation and barrier-related markers including filaggrin, caspase-14, and involucrin, and reduced hypoxia- and tissue-remodelling-associated mediators including HIF-1α, VEGF, COL6A3, and COL1A2. Network-based proteomic analyses further identified computationally inferred associations with SLIT/ROBO-related pathways, although these associations require further functional validation. Collectively, these findings support pantethine as a promising candidate that modulates vesicle-associated proteins together with inflammatory and tissue-remodelling responses involved in epidermal homeostasis.
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BibTeX
@article{sukphopetch2026pantethine,
title = {{Pantethine Restores Epidermal Homeostasis and Modulates Vesicle-Associated Proteins in Psoriasis.}},
author = {Passanesh Sukphopetch and Kamonpan Fongsodsri and Pornanong Aramwit and Onrapak Reamtong and Tipparat Thiangtrongjit and Tapanee Kanjanapruthipong and Watcharamat Muangkaew and Sumate Ampawong},
journal = {Experimental dermatology},
year = {2026},
month = oct,
doi = {10.1111/exd.70381},
url = {https://europepmc.org/article/MED/42963826},
note = {CC BY},
}
RIS
TY - JOUR
TI - Pantethine Restores Epidermal Homeostasis and Modulates Vesicle-Associated Proteins in Psoriasis.
AU - Passanesh Sukphopetch
AU - Kamonpan Fongsodsri
AU - Pornanong Aramwit
AU - Onrapak Reamtong
AU - Tipparat Thiangtrongjit
AU - Tapanee Kanjanapruthipong
AU - Watcharamat Muangkaew
AU - Sumate Ampawong
PY - 2026
DA - 2026/10/30
JO - Experimental dermatology
DO - 10.1111/exd.70381
UR - https://europepmc.org/article/MED/42963826
AB - Psoriasis is a chronic inflammatory skin disease characterized by disrupted epidermal homeostasis driven by interconnected immuno-inflammatory, metabolic, and redox signalling networks. Vesicle-associated intercellular communication has emerged as an important contributor to inflammatory amplification and tissue remodelling within the epidermal microenvironment. Pantethine, a stable vitamin B5 derivative with lipid-modulatory and anti-inflammatory properties, has been implicated in regulation of cellular stress responses and membrane-associated signalling pathways, suggesting potential therapeutic relevance in psoriasis. Here, we investigated the effects of pantethine using a full-thickness 3D human psoriatic skin model following keratinocyte safety assessment. Integrated histopathological, immunohistochemical, quantitative RT-PCR, and proteomic analyses were employed to interrogate inflammatory, oxidative, proliferative, apoptotic, differentiation-associated and vesicle-related responses. Pantethine significantly reduced epidermal thickening and normalized multiple psoriasis-associated molecular signatures. Proteomic profiling revealed coordinated modulation of vesicle-associated, cytoskeletal and extracellular matrix-related proteins, accompanied by reduced expression of exosome-associated markers CD63 and CD9. Pantethine suppressed pro-inflammatory signalling involving IL-1β, TNF-α, IL-17, S100 family members, and hyperproliferative keratin-associated pathways while reducing caspase-3-associated apoptotic activity. Concurrently, pantethine enhanced antioxidative and immunoregulatory responses through activation of Nrf-2 and TGF-β, restored differentiation and barrier-related markers including filaggrin, caspase-14, and involucrin, and reduced hypoxia- and tissue-remodelling-associated mediators including HIF-1α, VEGF, COL6A3, and COL1A2. Network-based proteomic analyses further identified computationally inferred associations with SLIT/ROBO-related pathways, although these associations require further functional validation. Collectively, these findings support pantethine as a promising candidate that modulates vesicle-associated proteins together with inflammatory and tissue-remodelling responses involved in epidermal homeostasis.
SN - 0906-6705
SN - 1600-0625
ER -