Time-Dependent UV/Ozone Treatment Modulates Platelet Adhesion on Polystyrene and Cyclo-Olefin Polymer through Fibrinogen Orientation.
- 1Department of Materials Science and Engineering, School of Materials and Chemical Technology, Institute of Science Tokyo, Yokohama226-8502, Japan.
- 2Institute for Materials Chemistry and Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka819-0395, Japan.
Abstract
UV/ozone (UVO) treatment is a versatile, solvent-free approach for modifying polymer surfaces in biomedical applications. Although its effects on RGD-dependent cell adhesion have been extensively studied, the mechanisms underlying non-RGD-dependent interactions, specifically platelet adhesion, remain poorly understood. In this study, we investigated the adhesion and activation of human platelets on polystyrene (PS) and cyclo-olefin polymer (COP) surfaces modified with 172 nm vacuum ultraviolet (VUV) light-induced UVO treatment. A distinctive non-monotonic relationship between UVO irradiation time and platelet response was observed: short-term treatment (1-2 min) maximized platelet density, with a substrate-dependent increase in platelet activation that was most pronounced on PS, whereas prolonged treatment (>10 min) slightly reduced platelet density compared to short-term treatment. Surface analyses by X-ray photoelectron spectroscopy (XPS), water contact angle (WCA), and atomic force microscopy (AFM) showed monotonic increases in oxygen content, hydrophilicity, and nanoscale roughness, and were accompanied by a two-orders-of-magnitude decrease in Young's modulus (from approximately 1500 MPa to 10 MPa). Quartz Crystal Microbalance with Dissipation (QCM-D) measurements of fibrinogen (Fg) adsorption indicated that short-term treatment promotes a "side-on" orientation of Fg, maximizing the exposure of γ-chain binding sites relevant to platelet integrin recognition. In contrast, long-term treatment leads to a crowded interfacial environment, likely disrupting stable protein presentation. Furthermore, excessive surface softening may impair platelet mechanosensing. These findings provide mechanistic insight into time-dependent UVO surface modification and offer a rational framework for optimizing the hemocompatibility of polymer-based medical devices.
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BibTeX
@article{kaizu2026timedependent,
title = {{Time-Dependent UV/Ozone Treatment Modulates Platelet Adhesion on Polystyrene and Cyclo-Olefin Polymer through Fibrinogen Orientation.}},
author = {Riko Kaizu and Aki Yamamoto and Masaru Tanaka and Tomohiro Hayashi},
journal = {Langmuir : the ACS journal of surfaces and colloids},
year = {2026},
month = sep,
doi = {10.1021/acs.langmuir.6c02349},
url = {https://europepmc.org/article/MED/42916823},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Time-Dependent UV/Ozone Treatment Modulates Platelet Adhesion on Polystyrene and Cyclo-Olefin Polymer through Fibrinogen Orientation.
AU - Riko Kaizu
AU - Aki Yamamoto
AU - Masaru Tanaka
AU - Tomohiro Hayashi
PY - 2026
DA - 2026/09/01
JO - Langmuir : the ACS journal of surfaces and colloids
DO - 10.1021/acs.langmuir.6c02349
UR - https://europepmc.org/article/MED/42916823
AB - UV/ozone (UVO) treatment is a versatile, solvent-free approach for modifying polymer surfaces in biomedical applications. Although its effects on RGD-dependent cell adhesion have been extensively studied, the mechanisms underlying non-RGD-dependent interactions, specifically platelet adhesion, remain poorly understood. In this study, we investigated the adhesion and activation of human platelets on polystyrene (PS) and cyclo-olefin polymer (COP) surfaces modified with 172 nm vacuum ultraviolet (VUV) light-induced UVO treatment. A distinctive non-monotonic relationship between UVO irradiation time and platelet response was observed: short-term treatment (1-2 min) maximized platelet density, with a substrate-dependent increase in platelet activation that was most pronounced on PS, whereas prolonged treatment (>10 min) slightly reduced platelet density compared to short-term treatment. Surface analyses by X-ray photoelectron spectroscopy (XPS), water contact angle (WCA), and atomic force microscopy (AFM) showed monotonic increases in oxygen content, hydrophilicity, and nanoscale roughness, and were accompanied by a two-orders-of-magnitude decrease in Young's modulus (from approximately 1500 MPa to 10 MPa). Quartz Crystal Microbalance with Dissipation (QCM-D) measurements of fibrinogen (Fg) adsorption indicated that short-term treatment promotes a "side-on" orientation of Fg, maximizing the exposure of γ-chain binding sites relevant to platelet integrin recognition. In contrast, long-term treatment leads to a crowded interfacial environment, likely disrupting stable protein presentation. Furthermore, excessive surface softening may impair platelet mechanosensing. These findings provide mechanistic insight into time-dependent UVO surface modification and offer a rational framework for optimizing the hemocompatibility of polymer-based medical devices.
SN - 0743-7463
SN - 1520-5827
ER -