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Peer-reviewedCC BY-NC-NDInfection prevention in practice

Molecular epidemiological analysis of a suspected nosocomial outbreak of carbapenem-resistant Klebsiella aerogenes.

  • Mahoko Ikeda1,2
  • Sachiko Hirose2
  • Satomi Takei3
  • Gene Igawa4
  • Satoshi Hori2,5
  • Yuki Uehara1,3
  • Akio Kanazawa1,2
  • Mai Suzuki1
  • Yoko Tabe3,4
  • Toshio Naito1
5 affiliations
  1. 1Department of General Medicine, Juntendo University Faculty of Medicine, Tokyo, Japan.
  2. 2Infection Prevention and Control Unit, Department of Infection Prevention and Control, Juntendo University Hospital, Tokyo, Japan.
  3. 3Department of Clinical Laboratory Medicine, Juntendo University Faculty of Medicine, Tokyo, Japan.
  4. 4Department of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan.
  5. 5Department of Infection Control Science, Juntendo University Graduate School of Medicine, Tokyo, Japan.
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Abstract

Objectives

Carbapenem-resistant Enterobacterales (CRE) are a common source of healthcare-associated infections and nosocomial outbreaks. We examined a suspected nosocomial outbreak of carbapenem-resistant Klebsiella aerogenes in a surgical ward over a period of 11 months.Methods

We used whole-genome sequencing (WGS) to assess the genetic relatedness of eight detected isolates.Results

Molecular epidemiological analyses confirmed the outbreak as a pseudo-outbreak. During the period of assessment, the hospital infection control team implemented multiple interventions to promote stringent hand hygiene compliance, appropriate use of personal protective equipment, and adherence to best practices for wound care and body fluid management. Despite these measures, intermittent CRE isolation continued. Notably, WGS analyses revealed that all assessed isolates were genetically unique, with no evidence to indicate clonal relatedness.Conclusions

The suspected nosocomial outbreak comprised sporadic, unrelated cases of CRE. Our findings in this study highlight the significance of molecular epidemiological tools in precisely evaluating suspected outbreaks of CRE and in directing the effective deployment of infection control resources.

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Cite

BibTeXRIS
BibTeX
@article{ikeda2026molecular,
  title = {{Molecular epidemiological analysis of a suspected nosocomial outbreak of carbapenem-resistant Klebsiella aerogenes.}},
  author = {Mahoko Ikeda and Sachiko Hirose and Satomi Takei and Gene Igawa and Satoshi Hori and Yuki Uehara and Akio Kanazawa and Mai Suzuki and Yoko Tabe and Toshio Naito},
  journal = {Infection prevention in practice},
  year = {2026},
  month = sep,
  doi = {10.1016/j.infpip.2026.100596},
  url = {https://europepmc.org/article/MED/42904077},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Molecular epidemiological analysis of a suspected nosocomial outbreak of carbapenem-resistant Klebsiella aerogenes.
AU  - Mahoko Ikeda
AU  - Sachiko Hirose
AU  - Satomi Takei
AU  - Gene Igawa
AU  - Satoshi Hori
AU  - Yuki Uehara
AU  - Akio Kanazawa
AU  - Mai Suzuki
AU  - Yoko Tabe
AU  - Toshio Naito
PY  - 2026
DA  - 2026/09/21
JO  - Infection prevention in practice
DO  - 10.1016/j.infpip.2026.100596
UR  - https://europepmc.org/article/MED/42904077
AB  - Objectives  Carbapenem-resistant Enterobacterales (CRE) are a common source of healthcare-associated infections and nosocomial outbreaks. We examined a suspected nosocomial outbreak of carbapenem-resistant Klebsiella aerogenes in a surgical ward over a period of 11 months.Methods  We used whole-genome sequencing (WGS) to assess the genetic relatedness of eight detected isolates.Results  Molecular epidemiological analyses confirmed the outbreak as a pseudo-outbreak. During the period of assessment, the hospital infection control team implemented multiple interventions to promote stringent hand hygiene compliance, appropriate use of personal protective equipment, and adherence to best practices for wound care and body fluid management. Despite these measures, intermittent CRE isolation continued. Notably, WGS analyses revealed that all assessed isolates were genetically unique, with no evidence to indicate clonal relatedness.Conclusions  The suspected nosocomial outbreak comprised sporadic, unrelated cases of CRE. Our findings in this study highlight the significance of molecular epidemiological tools in precisely evaluating suspected outbreaks of CRE and in directing the effective deployment of infection control resources.
SN  - 2590-0889
ER  -