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Peer-reviewedCC BY-NC-NDBMC plant biology

Rapid visual detection technologies for Botrytis cinerea resistance to QoI and SDHI fungicides based on RPA-CRISPR/Cas12a.

  • Yafeng Yuan1,2
  • Mei Liu1
  • Baoyu Wang1
  • Xuncheng Wang1
  • Qikai Xing1
  • Wenwen Liang1
  • Gan Wang1
  • Wei Zhang3,4
  • Jiye Yan5
5 affiliations
  1. 1Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
  2. 2College of Plant Protection, China Agricultural University, Beijing, 100193, China.
  3. 3Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. [email protected].
  4. 4College of Plant Protection, China Agricultural University, Beijing, 100193, China. [email protected].
  5. 5Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. [email protected].
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Abstract

Grape gray mold caused by Botrytis cinerea is one of the most important fungal diseases affecting grape production and postharvest quality. The extensive use of fungicides has led to the emergence of resistance in B. cinerea, particularly to quinone outside inhibitors (QoIs) and succinate dehydrogenase inhibitors (SDHI), which compromises the efficacy of disease control. Therefore, detection techniques for fungicide resistance in B. cinerea are needed to provide scientific guidance for disease management. Based on recombinase polymerase amplification (RPA)-CRISPR/Cas12a technology, this study designed specific RPA primer pairs and crRNA targeting resistance-related mutation sites in fungicide target genes cytb and SdhB, incorporated single-stranded fluorescent reporter molecules, and optimized key reaction components. This study achieved rapid fluorescence-based resistance detection within 1 h, with detection sensitivities of 450 and 430 pg/µL for the two fungicide genes.

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Cite

BibTeXRIS
BibTeX
@article{yuan2026rapid,
  title = {{Rapid visual detection technologies for Botrytis cinerea resistance to QoI and SDHI fungicides based on RPA-CRISPR/Cas12a.}},
  author = {Yafeng Yuan and Mei Liu and Baoyu Wang and Xuncheng Wang and Qikai Xing and Wenwen Liang and Gan Wang and Wei Zhang and Jiye Yan},
  journal = {BMC plant biology},
  year = {2026},
  month = aug,
  doi = {10.1186/s12870-026-09628-x},
  url = {https://europepmc.org/article/MED/42958025},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Rapid visual detection technologies for Botrytis cinerea resistance to QoI and SDHI fungicides based on RPA-CRISPR/Cas12a.
AU  - Yafeng Yuan
AU  - Mei Liu
AU  - Baoyu Wang
AU  - Xuncheng Wang
AU  - Qikai Xing
AU  - Wenwen Liang
AU  - Gan Wang
AU  - Wei Zhang
AU  - Jiye Yan
PY  - 2026
DA  - 2026/08/03
JO  - BMC plant biology
DO  - 10.1186/s12870-026-09628-x
UR  - https://europepmc.org/article/MED/42958025
AB  - Grape gray mold caused by Botrytis cinerea is one of the most important fungal diseases affecting grape production and postharvest quality. The extensive use of fungicides has led to the emergence of resistance in B. cinerea, particularly to quinone outside inhibitors (QoIs) and succinate dehydrogenase inhibitors (SDHI), which compromises the efficacy of disease control. Therefore, detection techniques for fungicide resistance in B. cinerea are needed to provide scientific guidance for disease management. Based on recombinase polymerase amplification (RPA)-CRISPR/Cas12a technology, this study designed specific RPA primer pairs and crRNA targeting resistance-related mutation sites in fungicide target genes cytb and SdhB, incorporated single-stranded fluorescent reporter molecules, and optimized key reaction components. This study achieved rapid fluorescence-based resistance detection within 1 h, with detection sensitivities of 450 and 430 pg/µL for the two fungicide genes.
SN  - 1471-2229
ER  -