Proteomic and Molecular Biological Analyses of Response Mechanism in Rice (Oryza sativa L. cv. Koshihikari) Under Drought Stress
4 affiliations
- 1Department of Applied Sciences and Engineering, Fukui University of Technology, Fukui 910-8505, Japan
- 2Department of Management Nutrition, Aichi Gakusen University, Okazaki 444-8520, Japan
- 3Center for Medical Science, Fujita Health University, Toyoake 470-1192, Japan
- 4Faculty of Environment and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan
Abstract
Drought stress severely limits rice growth, while roots play a key role in sensing water deficits and triggering adaptive responses. To investigate time-dependent molecular responses in rice (Oryza sativa L. cv. Koshihikari) under drought stress induced by polyethylene glycol (PEG), seedlings were exposed to 10 and 20% PEG for 4 and 8 days, followed by morphological, proteomic, immunoblot, polymerase chain reaction, and ATP content analyses. Primary root length and total root fresh weight were significantly decreased with 20% PEG for 8 days compared with untreated. Proteomic analysis revealed changes in the abundance of rice root proteins under 20% PEG treatment between 4 and 8 days, with differentially abundant proteins enriched in Gene Ontology categories related to nuclear components, protein phosphorylation, and ATP binding. Selected proteins related to protein protection, redox-associated processes, and cytoskeletal responses were examined using immunoblot and polymerase chain reaction analyses. Glutathione S-transferase decreased and heat shock protein 70 increased with PEG for 8 days. The expression of NADPH-dependent codeinone reductase was upregulated and heat shock protein 17.9 was downregulated by PEG for 8 days. ATP content per unit fresh weight decreased in roots after PEG treatment. These results suggest that prolonged PEG-induced osmotic stress inhibits rice root growth through alterations in cellular energy status, the abundance of proteins involved in protein protection and glutathione S-transferase-mediated detoxification, and the expression of genes associated with reactive carbonyl species metabolism.
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BibTeX
@article{shohag2026proteomic,
title = {{Proteomic and Molecular Biological Analyses of Response Mechanism in Rice (Oryza sativa L. cv. Koshihikari) Under Drought Stress}},
author = {Sheikh Shohag and Hisateru Yamaguchi and Keisuke Hitachi and Kunihiro Tsuchida and Setsuko Komatsu},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27198895},
url = {https://www.mdpi.com/1422-0067/27/19/8895},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Proteomic and Molecular Biological Analyses of Response Mechanism in Rice (Oryza sativa L. cv. Koshihikari) Under Drought Stress
AU - Sheikh Shohag
AU - Hisateru Yamaguchi
AU - Keisuke Hitachi
AU - Kunihiro Tsuchida
AU - Setsuko Komatsu
PY - 2026
DA - 2026/10/07
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27198895
UR - https://www.mdpi.com/1422-0067/27/19/8895
AB - Drought stress severely limits rice growth, while roots play a key role in sensing water deficits and triggering adaptive responses. To investigate time-dependent molecular responses in rice (Oryza sativa L. cv. Koshihikari) under drought stress induced by polyethylene glycol (PEG), seedlings were exposed to 10 and 20% PEG for 4 and 8 days, followed by morphological, proteomic, immunoblot, polymerase chain reaction, and ATP content analyses. Primary root length and total root fresh weight were significantly decreased with 20% PEG for 8 days compared with untreated. Proteomic analysis revealed changes in the abundance of rice root proteins under 20% PEG treatment between 4 and 8 days, with differentially abundant proteins enriched in Gene Ontology categories related to nuclear components, protein phosphorylation, and ATP binding. Selected proteins related to protein protection, redox-associated processes, and cytoskeletal responses were examined using immunoblot and polymerase chain reaction analyses. Glutathione S-transferase decreased and heat shock protein 70 increased with PEG for 8 days. The expression of NADPH-dependent codeinone reductase was upregulated and heat shock protein 17.9 was downregulated by PEG for 8 days. ATP content per unit fresh weight decreased in roots after PEG treatment. These results suggest that prolonged PEG-induced osmotic stress inhibits rice root growth through alterations in cellular energy status, the abundance of proteins involved in protein protection and glutathione S-transferase-mediated detoxification, and the expression of genes associated with reactive carbonyl species metabolism.
SN - 1422-0067
ER -