Antibiofilm and Quorum-sensing Agents Against Pseudomonas aeruginosa and Staphylococcus aureus: a Systematic Review of Preclinical Studies, 2022-2026
- 1Department of Medical Laboratory Sciences, Faculty of Science, Al-Balqa Applied University, Al-Salt, Jordan.
Abstract
Background: Biofilm formation and quorum sensing (QS) make Pseudomonas aeruginosa and Staphylococcus aureus hard to treat, and many preclinical agents are reported to act against both.
Objective: We mapped preclinical antibiofilm and anti-QS agents against these pathogens and graded how directly each study tested QS.
Methods: PubMed and Scopus were searched without a date limit (3,349 records). Eligible in vitro and in vivo studies were published from 2022 onward, tested an exogenous agent and named either organism in the title. Full-text data were extracted, each study received a QS evidence tier, and risk of bias was appraised with an adapted checklist (in vitro) or SYRCLE's tool (vertebrate studies); two reviewers verified the extraction.
Results: We included 252 studies, led by natural products (115). A QS assay in the target pathogen was present in 140 studies (56%), a surrogate reporter alone in 27 (11%), and 31 (12%) used a vertebrate infection model. In vitro risk of bias was low in 143, moderate in 93 and high in 16. No meta-analysis was done, and title-only organism matching may have missed studies.
Conclusion: Direct QS evidence is common for P. aeruginosa but thinner for S. aureus, and animal and cytotoxicity data are sparse.
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BibTeX
@article{maaitah2026antibiofilm,
title = {{Antibiofilm and Quorum-sensing Agents Against Pseudomonas aeruginosa and Staphylococcus aureus: a Systematic Review of Preclinical Studies, 2022-2026}},
author = {Sham Ma’aitah and Batool AlAmairyin},
journal = {Journal of Basic and Applied Research in Biomedicine},
year = {2026},
month = oct,
doi = {10.51152/jbarbiomed.v12i1.267},
url = {https://doi.org/10.51152/jbarbiomed.v12i1.267},
note = {CC BY},
}
RIS
TY - JOUR
TI - Antibiofilm and Quorum-sensing Agents Against Pseudomonas aeruginosa and Staphylococcus aureus: a Systematic Review of Preclinical Studies, 2022-2026
AU - Sham Ma’aitah
AU - Batool AlAmairyin
PY - 2026
DA - 2026/10/01
JO - Journal of Basic and Applied Research in Biomedicine
DO - 10.51152/jbarbiomed.v12i1.267
UR - https://doi.org/10.51152/jbarbiomed.v12i1.267
AB - Background: Biofilm formation and quorum sensing (QS) make Pseudomonas aeruginosa and Staphylococcus aureus hard to treat, and many preclinical agents are reported to act against both. Objective: We mapped preclinical antibiofilm and anti-QS agents against these pathogens and graded how directly each study tested QS. Methods: PubMed and Scopus were searched without a date limit (3,349 records). Eligible in vitro and in vivo studies were published from 2022 onward, tested an exogenous agent and named either organism in the title. Full-text data were extracted, each study received a QS evidence tier, and risk of bias was appraised with an adapted checklist (in vitro) or SYRCLE's tool (vertebrate studies); two reviewers verified the extraction. Results: We included 252 studies, led by natural products (115). A QS assay in the target pathogen was present in 140 studies (56%), a surrogate reporter alone in 27 (11%), and 31 (12%) used a vertebrate infection model. In vitro risk of bias was low in 143, moderate in 93 and high in 16. No meta-analysis was done, and title-only organism matching may have missed studies. Conclusion: Direct QS evidence is common for P. aeruginosa but thinner for S. aureus, and animal and cytotoxicity data are sparse.
SN - 2710-1479
ER -