Xinjiang Brown Cattle-Derived β-Defensin EBD Alleviates Enterococcus faecalis-Induced Mammary Inflammation in Mice
7 affiliations
- 1College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China
- 2Urumqi Field Scientific Observation and Research Station for Animal Diseases, Ministry of Agriculture and Rural Affairs, Urumqi 830052, China
- 3Xinjiang Key Laboratory of New Drug Research and Development for Herbivorous Animals, Urumqi 830052, China
- 4Xinjiang Regional Key Laboratory of Clinical Veterinary Medicine Research, Urumqi 830052, China
- 5Herbivorous Animal Biomedicine and Diagnostic Technology Engineering Research Center, Xinjiang Uygur Autonomous Region, Urumqi 830052, China
- 6College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
- 7State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi 830011, China
Abstract
Bovine mastitis is a major infectious disease in dairy cattle, and the increasing prevalence of antimicrobial-resistant pathogens has created an urgent need for the development of effective antimicrobial agents. In this study, bovine enteric β-defensin (EBD) from Xinjiang Brown Cattle was cloned, expressed, and preliminarily evaluated for its antibacterial and protective effects against Enterococcus faecalis-induced mastitis. Bioinformatic analysis identified EBD as a secreted protein belonging to the bovine β-defensin family. The recombinant PCDH-EBD construct was successfully transfected into 293T cells, resulting in markedly increased EBD mRNA expression and detectable recombinant EBD protein expression, as confirmed by SDS-PAGE and Western blotting. In vitro agar diffusion assays showed that the EBD-containing preparation exhibited inhibitory activity against E. faecalis. In a murine mastitis model, EBD treatment significantly attenuated mammary histopathological lesions and reduced the expression of Mpo, Tnf, and Il6 compared with the mastitis group. 16S rRNA sequencing of mammary gland tissues showed that EBD treatment was associated with a reduced relative abundance of Enterococcus and partial restoration of microbial diversity. Transcriptomic analysis further revealed that EBD treatment was associated with alterations in NOD-like receptor signaling- and phagosome-related pathways, suggesting that these processes may contribute to its protective effects. Collectively, these findings indicate that enteric β-defensin EBD from Xinjiang Brown Cattle possesses antibacterial and anti-inflammatory potential, making it a candidate for developing antimicrobial agents against bovine mastitis.
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BibTeX
@article{song2026xinjiang,
title = {{Xinjiang Brown Cattle-Derived β-Defensin EBD Alleviates Enterococcus faecalis-Induced Mammary Inflammation in Mice}},
author = {Yaolong Song and Yingchao Li and Mingshuai Chen and Chenyang Shi and Qirui Zang and Wanjing Jin and Qian Ma and Binhuan Ma and Panpan Tong and Shicheng Wan and Aili Aierken and Mengfei Zhang},
journal = {Cells},
year = {2026},
month = oct,
doi = {10.3390/cells15211980},
url = {https://www.mdpi.com/2073-4409/15/21/1980},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Xinjiang Brown Cattle-Derived β-Defensin EBD Alleviates Enterococcus faecalis-Induced Mammary Inflammation in Mice
AU - Yaolong Song
AU - Yingchao Li
AU - Mingshuai Chen
AU - Chenyang Shi
AU - Qirui Zang
AU - Wanjing Jin
AU - Qian Ma
AU - Binhuan Ma
AU - Panpan Tong
AU - Shicheng Wan
AU - Aili Aierken
AU - Mengfei Zhang
PY - 2026
DA - 2026/10/30
JO - Cells
DO - 10.3390/cells15211980
UR - https://www.mdpi.com/2073-4409/15/21/1980
AB - Bovine mastitis is a major infectious disease in dairy cattle, and the increasing prevalence of antimicrobial-resistant pathogens has created an urgent need for the development of effective antimicrobial agents. In this study, bovine enteric β-defensin (EBD) from Xinjiang Brown Cattle was cloned, expressed, and preliminarily evaluated for its antibacterial and protective effects against Enterococcus faecalis-induced mastitis. Bioinformatic analysis identified EBD as a secreted protein belonging to the bovine β-defensin family. The recombinant PCDH-EBD construct was successfully transfected into 293T cells, resulting in markedly increased EBD mRNA expression and detectable recombinant EBD protein expression, as confirmed by SDS-PAGE and Western blotting. In vitro agar diffusion assays showed that the EBD-containing preparation exhibited inhibitory activity against E. faecalis. In a murine mastitis model, EBD treatment significantly attenuated mammary histopathological lesions and reduced the expression of Mpo, Tnf, and Il6 compared with the mastitis group. 16S rRNA sequencing of mammary gland tissues showed that EBD treatment was associated with a reduced relative abundance of Enterococcus and partial restoration of microbial diversity. Transcriptomic analysis further revealed that EBD treatment was associated with alterations in NOD-like receptor signaling- and phagosome-related pathways, suggesting that these processes may contribute to its protective effects. Collectively, these findings indicate that enteric β-defensin EBD from Xinjiang Brown Cattle possesses antibacterial and anti-inflammatory potential, making it a candidate for developing antimicrobial agents against bovine mastitis.
SN - 2073-4409
ER -