Bioprocess optimization of
4 affiliations
- 1Agroscope, Department of Food Microbial Systems, Posieux, 1725, Switzerland; ETH Zürich, Institute of Food, Nutrition, and Health, Department of Health Sciences and Technology, Zurich, 8092, Switzerland; Corresponding authors at: Agroscope, Department of Food Microbial Systems, Posieux, 1725, Switzerland.
- 2ETH Zürich, Institute of Food, Nutrition, and Health, Department of Health Sciences and Technology, Zurich, 8092, Switzerland
- 3Agroscope, Department of Plant Protection, Nyon, 1260, Switzerland
- 4Agroscope, Department of Food Microbial Systems, Posieux, 1725, Switzerland; Corresponding authors at: Agroscope, Department of Food Microbial Systems, Posieux, 1725, Switzerland.
Abstract
Although Metschnikowia pulcherrima shows promise as a biocontrol agent, its upstream development remains limited. We maximized the viable cell count of M. pulcherrima strain APC 1.2 by employing a Central Composite Design (CCD) to optimize pH and temperature. Preliminary screening identified sucrose at 20 g/L and 1600 mg YAN/L yeast extract as optimal for viable cell count (CFU/mL), with further increases in YAN providing no additional benefit. The highest viable cell count was achieved at milder temperatures (18–22 °C), where the strain grew robustly across a wide pH range (3.59–6.41), while higher temperatures increased sensitivity to acidic pH and reduced viable cell count. The strain was confirmed to be Crabtree-negative, showing no ethanol production during aerobic growth on glucose. The proposed workflow enabled reproducible optimization of cultivation parameters, providing a scalable framework for industrial applications, and laying the groundwork for future studies on non-conventional yeasts in biocontrol and industrial biotechnology.
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BibTeX
@article{dinic2026bioprocess,
title = {{Bioprocess optimization of}},
author = {Bogdan T. Dinić and Nicholas A. Bokulich and Florian M. Freimoser and Ueli von Ah},
journal = {Biotechnology Reports},
year = {2026},
month = oct,
doi = {10.1016/j.btre.2026.e00984},
url = {https://doi.org/10.1016/j.btre.2026.e00984},
note = {CC BY},
}
RIS
TY - JOUR
TI - Bioprocess optimization of
AU - Bogdan T. Dinić
AU - Nicholas A. Bokulich
AU - Florian M. Freimoser
AU - Ueli von Ah
PY - 2026
DA - 2026/10/10
JO - Biotechnology Reports
DO - 10.1016/j.btre.2026.e00984
UR - https://doi.org/10.1016/j.btre.2026.e00984
AB - Although Metschnikowia pulcherrima shows promise as a biocontrol agent, its upstream development remains limited. We maximized the viable cell count of M. pulcherrima strain APC 1.2 by employing a Central Composite Design (CCD) to optimize pH and temperature. Preliminary screening identified sucrose at 20 g/L and 1600 mg YAN/L yeast extract as optimal for viable cell count (CFU/mL), with further increases in YAN providing no additional benefit. The highest viable cell count was achieved at milder temperatures (18–22 °C), where the strain grew robustly across a wide pH range (3.59–6.41), while higher temperatures increased sensitivity to acidic pH and reduced viable cell count. The strain was confirmed to be Crabtree-negative, showing no ethanol production during aerobic growth on glucose. The proposed workflow enabled reproducible optimization of cultivation parameters, providing a scalable framework for industrial applications, and laying the groundwork for future studies on non-conventional yeasts in biocontrol and industrial biotechnology.
SN - 2215-017X
ER -