A biosensor toolbox for monitoring recombinant protein production in Escherichia coli.
- 1Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
- 2The Novo Nordisk Foundation Biotechnology Research Institute for the Green Transition (BRIGHT), Technical University of Denmark, Lyngby, Denmark.
Abstract
Recombinant protein production can be a challenging process. To help users troubleshoot and optimize experiments in Escherichia coli we have compiled and characterized a toolbox of biosensor plasmids. It contains a biosensor that monitors the translational efficiency of the recombinant protein and quality control biosensors that detect unwanted events, such as protein misfolding in the cytoplasm, inefficient translocation through the Sec translocon, or protein misfolding in the periplasm. The biosensors were combined with different fluorescent proteins so that multiple events could be measured in parallel, in the same cell. The toolbox is a resource for diagnosing protein production problems and evaluating solutions that mitigate these problems.
Related
- An L-threonine-inducible biosensor derived from the tryptophan-repressed trp promoter of Escherichia coli for high-throughput screening of L-threonine overproducers
- The Tryptophan Operator Acts as a Portable Downstream Enhancer for Recombinant Protein Expression in Escherichia coli
- Comparison of Wildtype and Recombinant Adeno-Associated Virus (AAV) Production Reveals Distinct Productivity and Content Ratio Profiles.
- Recombinant bacterial β-galactosidases for lactose bioconversion and galacto-oligosaccharide production: a comparative overview
- Streamlined Production of Recombinant Adeno-Associated Viruses Using Gateway Cloning Technology for Neural Circuit All-Optical Interrogation
BibTeX
@article{murkoster2026biosensor,
title = {{A biosensor toolbox for monitoring recombinant protein production in Escherichia coli.}},
author = {Maurice Mürköster and Mateusz Balka and Christina J Kamp and Judit Källström and Linus Karlsson and Claudia A Rodriguez Aparicio and Erik Tunäng and Hanna-Louise Ögren and Silvia Theopold and Alister J Cumming and Alex Toftgaard Nielsen and Daniel O Daley},
journal = {Frontiers in bioengineering and biotechnology},
year = {2026},
month = oct,
doi = {10.3389/fbioe.2026.1956994},
url = {https://europepmc.org/article/MED/42909106},
note = {CC BY},
}
RIS
TY - JOUR
TI - A biosensor toolbox for monitoring recombinant protein production in Escherichia coli.
AU - Maurice Mürköster
AU - Mateusz Balka
AU - Christina J Kamp
AU - Judit Källström
AU - Linus Karlsson
AU - Claudia A Rodriguez Aparicio
AU - Erik Tunäng
AU - Hanna-Louise Ögren
AU - Silvia Theopold
AU - Alister J Cumming
AU - Alex Toftgaard Nielsen
AU - Daniel O Daley
PY - 2026
DA - 2026/10/07
JO - Frontiers in bioengineering and biotechnology
DO - 10.3389/fbioe.2026.1956994
UR - https://europepmc.org/article/MED/42909106
AB - Recombinant protein production can be a challenging process. To help users troubleshoot and optimize experiments in Escherichia coli we have compiled and characterized a toolbox of biosensor plasmids. It contains a biosensor that monitors the translational efficiency of the recombinant protein and quality control biosensors that detect unwanted events, such as protein misfolding in the cytoplasm, inefficient translocation through the Sec translocon, or protein misfolding in the periplasm. The biosensors were combined with different fluorescent proteins so that multiple events could be measured in parallel, in the same cell. The toolbox is a resource for diagnosing protein production problems and evaluating solutions that mitigate these problems.
SN - 2296-4185
ER -