Optimization of ultrasound-assisted extraction of polyphenols from Mansoa alliacea flowers and evaluation of their antioxidant, anti-inflammatory-related, and digestive enzyme inhibitory activities.
- 1Faculty of Chemistry, University of Science, Ho Chi Minh City, 700000, Viet Nam.
- 2Vietnam National University, Ho Chi Minh City, 700000, Viet Nam.
- 3Faculty of Medicine, College of Health Sciences, Nam Can Tho University, 168 Nguyen Van Cu Extension Street, An Binh Ward, Can Tho, 94000, Viet Nam.
Abstract
Mansoa alliacea is traditionally used to treat inflammation, infections, and metabolic disorders. However, the phytochemical and biological potential of its flowers remains poorly characterized. This study optimized ultrasound-assisted extraction (UAE) of polyphenols from M. alliacea flowers and evaluated the in vitro antioxidant, anti-inflammatory-related, and digestive enzyme inhibitory activities of the optimized extract. Four extraction parameters (ethanol concentration, extraction temperature, extraction time, and solid-to-solvent ratio) were initially screened by single-factor experiments. Based on these results, three key factors (temperature, time, and ethanol concentration) were selected and further optimized using a Box-Behnken design to maximize polyphenol yield. Total polyphenol content was quantified using the Folin-Ciocalteu method. UHPLC-MS profiling was conducted for tentative phytochemical characterization. The optimized extract was evaluated for in vitro antioxidant activity (DPPH, ABTS assays), anti-inflammatory activity (protein denaturation and RBC membrane stabilization), and digestive enzyme inhibitory activities (inhibition of α-amylase and α-glucosidase). Optimal extraction conditions (60% ethanol, 79 °C, 15 min, 1:10 w/v) yielded 275.37 ± 1.55 mg GAE/g extract. UHPLC-MS analysis revealed nine major peaks, among which two compounds were provisionally assigned as luteolin-7-O-glucoside and luteolin-7-O-glucuronide. Compared with the unoptimized extract, the optimized extract exhibited markedly stronger in vitro biological activities, with IC50 values of 11.88 ± 0.28 and 15.58 ± 0.33 μg/mL in DPPH and ABTS assays, respectively; 38.77 ± 0.93 μg/mL for inhibition of protein denaturation; 30.74 ± 0.18 μg/mL for RBC membrane stabilization; and 30.94 ± 0.54 and 13.32 ± 0.19 μg/mL for α-amylase and α-glucosidase inhibition, respectively. Optimized UAE significantly enhanced polyphenol enrichment in the extract and improved the in vitro antioxidant capacity, protective effects against protein denaturation and RBC hemolysis, and carbohydrate-hydrolyzing enzyme inhibitory activities of M. alliacea flower extract. These findings provide preliminary scientific support for the traditional use of M. alliacea and suggest that its polyphenol-rich extract may serve as a promising candidate for further investigation as a functional or phytopharmaceutical ingredient.
Related
- Ultrasound-Assisted Deep Eutectic Solvent Extraction of Flavonoids from Hovenia dulcis Thunb: Optimization, Phytochemical Profiling, and Antioxidant and Anti-Inflammatory Activity Evaluation.
- Efficient Extraction of Polyphenols from Hamamelis virginiana Bark and Leaves: A Design-of-Experiments Approach to Accelerated Solvent- and Ultrasound-Assisted Extraction
- Acoustic cavitation-mediated ultrasound-assisted extraction enhances recovery of antioxidant and antidiabetic phytochemicals from Tanacetum gracile: Stabilization through alginate–whey protein microencapsulation
- PEG 200-Assisted Ultrasound Extraction and Characterization of Mulberry Anthocyanins: Optimization, Bioactivities, and Stability.
- Application of Ultrasound-Assisted Extraction for Isolating Rhamnetin from Clove (Syzygium aromaticum) Flower Buds: Process Optimization, Identification, and Bioactivity Assessment
BibTeX
@article{nguyen2026optimization,
title = {{Optimization of ultrasound-assisted extraction of polyphenols from Mansoa alliacea flowers and evaluation of their antioxidant, anti-inflammatory-related, and digestive enzyme inhibitory activities.}},
author = {Quoc Trung Nguyen and Tran Chi Linh},
journal = {Biochemistry and biophysics reports},
year = {2026},
month = oct,
doi = {10.1016/j.bbrep.2026.102851},
url = {https://europepmc.org/article/MED/42999665},
note = {CC BY},
}
RIS
TY - JOUR
TI - Optimization of ultrasound-assisted extraction of polyphenols from Mansoa alliacea flowers and evaluation of their antioxidant, anti-inflammatory-related, and digestive enzyme inhibitory activities.
AU - Quoc Trung Nguyen
AU - Tran Chi Linh
PY - 2026
DA - 2026/10/31
JO - Biochemistry and biophysics reports
DO - 10.1016/j.bbrep.2026.102851
UR - https://europepmc.org/article/MED/42999665
AB - Mansoa alliacea is traditionally used to treat inflammation, infections, and metabolic disorders. However, the phytochemical and biological potential of its flowers remains poorly characterized. This study optimized ultrasound-assisted extraction (UAE) of polyphenols from M. alliacea flowers and evaluated the in vitro antioxidant, anti-inflammatory-related, and digestive enzyme inhibitory activities of the optimized extract. Four extraction parameters (ethanol concentration, extraction temperature, extraction time, and solid-to-solvent ratio) were initially screened by single-factor experiments. Based on these results, three key factors (temperature, time, and ethanol concentration) were selected and further optimized using a Box-Behnken design to maximize polyphenol yield. Total polyphenol content was quantified using the Folin-Ciocalteu method. UHPLC-MS profiling was conducted for tentative phytochemical characterization. The optimized extract was evaluated for in vitro antioxidant activity (DPPH, ABTS assays), anti-inflammatory activity (protein denaturation and RBC membrane stabilization), and digestive enzyme inhibitory activities (inhibition of α-amylase and α-glucosidase). Optimal extraction conditions (60% ethanol, 79 °C, 15 min, 1:10 w/v) yielded 275.37 ± 1.55 mg GAE/g extract. UHPLC-MS analysis revealed nine major peaks, among which two compounds were provisionally assigned as luteolin-7-O-glucoside and luteolin-7-O-glucuronide. Compared with the unoptimized extract, the optimized extract exhibited markedly stronger in vitro biological activities, with IC50 values of 11.88 ± 0.28 and 15.58 ± 0.33 μg/mL in DPPH and ABTS assays, respectively; 38.77 ± 0.93 μg/mL for inhibition of protein denaturation; 30.74 ± 0.18 μg/mL for RBC membrane stabilization; and 30.94 ± 0.54 and 13.32 ± 0.19 μg/mL for α-amylase and α-glucosidase inhibition, respectively. Optimized UAE significantly enhanced polyphenol enrichment in the extract and improved the in vitro antioxidant capacity, protective effects against protein denaturation and RBC hemolysis, and carbohydrate-hydrolyzing enzyme inhibitory activities of M. alliacea flower extract. These findings provide preliminary scientific support for the traditional use of M. alliacea and suggest that its polyphenol-rich extract may serve as a promising candidate for further investigation as a functional or phytopharmaceutical ingredient.
SN - 2405-5808
ER -