Exploratory Analysis of the Innate Immune System in Aortic Stenosis Patients With and Without ASCVD.
10 affiliations
- 1Department of Cardiology, Radboud University Medical Center, Nijmegen, The Netherlands.
- 2Department of Cardiology, Radboud University Medical Center, Nijmegen, The Netherlands; Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
- 3Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
- 4Department of IQ Health, Radboud University Medical Center, Nijmegen, The Netherlands.
- 5Acibadem Heart Center, Acibadem International Medical Center, Rotterdam, The Netherlands.
- 6Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands; Department of Immunology and Metabolism, Life and Medical Sciences Center, University of Bonn, Bonn, Germany.
- 7Amsterdam Cardiovascular Sciences, Amsterdam, The Netherlands; Department of Cardiac Surgery, Amsterdam University Medical Center, Amsterdam, The Netherlands.
- 8Amsterdam Cardiovascular Sciences, Amsterdam, The Netherlands; Department of Pathology, Amsterdam University Medical Center, Amsterdam, The Netherlands.
- 9Department of Cardiology, Radboud University Medical Center, Nijmegen, The Netherlands; Dutch Network for Cardiovascular Research (WCN), Utrecht, The Netherlands; Department of Cardiology, Northwest Clinics, Alkmaar, The Netherlands.
- 10Department of Cardiology, Radboud University Medical Center, Nijmegen, The Netherlands. Electronic address: [email protected].
Abstract
Calcific aortic valve disease (CAVD) leads to aortic stenosis (AS) through inflammation and fibro-calcific remodeling. Although CAVD and atherosclerosis share features, not all patients with AS develop atherosclerotic cardiovascular disease (ASCVD), suggesting mechanistic heterogeneity. This case-control study evaluated inflammatory profiles and valvular remodeling in 128 patients with AS (59.4% with ASCVD) and 105 controls. Immune cell composition and monocyte markers were analyzed by flow cytometry, whereas cytokine production was assessed after peripheral blood mononuclear cell (PBMC) stimulation. Both AS groups had higher interleukin (IL)-6, leukocyte, monocyte, and neutrophil counts than controls, with lower monocyte human leukocyte antigen-DR isotype and higher C-C chemokine receptor (CCR)2 expression. Cytokine responses were also elevated, particularly in AS patients with ASCVD. They also showed higher IL-6 concentrations, higher proportions of classical monocytes, and lower intermediate and non-classical monocytes compared with patients without ASCVD. Overall, AS was associated with a pro-inflammatory monocyte phenotype and function, most prominently in patients with ASCVD. ClinicalTrials.gov ID: NCT04717219.
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BibTeX
@article{broekhoven2026exploratory,
title = {{Exploratory Analysis of the Innate Immune System in Aortic Stenosis Patients With and Without ASCVD.}},
author = {Amber van Broekhoven and Eveline P M van Doorn and Wieteke Broeders and Aysun Cetinyurek-Yavuz and Erwin S Zegers and Mihai G Netea and Alexander B A Vonk and Niels van Royen and Hans W M Niessen and Jan H Cornel and Niels P Riksen and Saloua El Messaoudi},
journal = {JACC. Basic to translational science},
year = {2026},
month = oct,
doi = {10.1016/j.jacbts.2026.101692},
url = {https://europepmc.org/article/MED/42874162},
note = {CC BY},
}
RIS
TY - JOUR
TI - Exploratory Analysis of the Innate Immune System in Aortic Stenosis Patients With and Without ASCVD.
AU - Amber van Broekhoven
AU - Eveline P M van Doorn
AU - Wieteke Broeders
AU - Aysun Cetinyurek-Yavuz
AU - Erwin S Zegers
AU - Mihai G Netea
AU - Alexander B A Vonk
AU - Niels van Royen
AU - Hans W M Niessen
AU - Jan H Cornel
AU - Niels P Riksen
AU - Saloua El Messaoudi
PY - 2026
DA - 2026/10/14
JO - JACC. Basic to translational science
DO - 10.1016/j.jacbts.2026.101692
UR - https://europepmc.org/article/MED/42874162
AB - Calcific aortic valve disease (CAVD) leads to aortic stenosis (AS) through inflammation and fibro-calcific remodeling. Although CAVD and atherosclerosis share features, not all patients with AS develop atherosclerotic cardiovascular disease (ASCVD), suggesting mechanistic heterogeneity. This case-control study evaluated inflammatory profiles and valvular remodeling in 128 patients with AS (59.4% with ASCVD) and 105 controls. Immune cell composition and monocyte markers were analyzed by flow cytometry, whereas cytokine production was assessed after peripheral blood mononuclear cell (PBMC) stimulation. Both AS groups had higher interleukin (IL)-6, leukocyte, monocyte, and neutrophil counts than controls, with lower monocyte human leukocyte antigen-DR isotype and higher C-C chemokine receptor (CCR)2 expression. Cytokine responses were also elevated, particularly in AS patients with ASCVD. They also showed higher IL-6 concentrations, higher proportions of classical monocytes, and lower intermediate and non-classical monocytes compared with patients without ASCVD. Overall, AS was associated with a pro-inflammatory monocyte phenotype and function, most prominently in patients with ASCVD. ClinicalTrials.gov ID: NCT04717219.
SN - 2452-302X
ER -