Stereospecific Characterization of Triacylglycerols in Vegetable Oils by Chiral Supercritical Fluid Chromatography and Mass Spectrometry
- 1Food Sciences Unit, Department of Life Technologies, University of Turku, FI-20014 Turku, Finland
Abstract
Although triacylglycerols (TGs) in vegetable oils are comparatively well characterized at the molecular species level, the detailed stereospecific composition and species-dependent variation remain largely unexplored. This study examined the separation and chiral elution behavior of eight asymmetric TG molecular species using supercritical fluid chromatography mass spectrometry (SFC-MS) with two CHIRALPAK IG-U columns and an acetonitrile/methanol modifier. Following evaluation using TG standards, the chiral SFC-MS method was applied to separate and characterize regioisomers and enantiomers of five asymmetric TG molecular species containing both saturated and unsaturated fatty acyls across 12 vegetable oils. Stereospecific profiles of these TG molecular species varied markedly among plant species, revealing nonrandom patterns and interspecies differences in fatty acyl distribution within the glycerol backbone of TGs. These findings contribute to understanding the chirality in TG biosynthesis in plants and offer valuable information to support targeted food formulation and product development.
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BibTeX
@article{kalpio2026stereospecific,
title = {{Stereospecific Characterization of Triacylglycerols in Vegetable Oils by Chiral Supercritical Fluid Chromatography and Mass Spectrometry}},
author = {Marika Kalpio and Dilakshi Naotunna Palliya Guruge and Mikael Fabritius and Qizhu Zhao and Baoru Yang},
journal = {Journal of Agricultural and Food Chemistry},
year = {2026},
month = oct,
doi = {10.1021/acs.jafc.6c07006},
url = {https://doi.org/10.1021/acs.jafc.6c07006},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Stereospecific Characterization of Triacylglycerols in Vegetable Oils by Chiral Supercritical Fluid Chromatography and Mass Spectrometry
AU - Marika Kalpio
AU - Dilakshi Naotunna Palliya Guruge
AU - Mikael Fabritius
AU - Qizhu Zhao
AU - Baoru Yang
PY - 2026
DA - 2026/10/24
JO - Journal of Agricultural and Food Chemistry
DO - 10.1021/acs.jafc.6c07006
UR - https://doi.org/10.1021/acs.jafc.6c07006
AB - Although triacylglycerols (TGs) in vegetable oils are comparatively well characterized at the molecular species level, the detailed stereospecific composition and species-dependent variation remain largely unexplored. This study examined the separation and chiral elution behavior of eight asymmetric TG molecular species using supercritical fluid chromatography mass spectrometry (SFC-MS) with two CHIRALPAK IG-U columns and an acetonitrile/methanol modifier. Following evaluation using TG standards, the chiral SFC-MS method was applied to separate and characterize regioisomers and enantiomers of five asymmetric TG molecular species containing both saturated and unsaturated fatty acyls across 12 vegetable oils. Stereospecific profiles of these TG molecular species varied markedly among plant species, revealing nonrandom patterns and interspecies differences in fatty acyl distribution within the glycerol backbone of TGs. These findings contribute to understanding the chirality in TG biosynthesis in plants and offer valuable information to support targeted food formulation and product development.
SN - 0021-8561
SN - 1520-5118
ER -