Host-triggered prophage induction drives Lactiplantibacillus plantarum symbiosis through bacterial lysis

Abstract

The release of bacterial bioactive molecules across the gut barrier is a crucial yet poorly understood step in host-microbe communication. Here, we identify a phage-driven mechanism associated with this process in the beneficial symbiont Lactiplantibacillus plantarum NC8 (LpNC8). We show that a stress-responsive prophage, pp2, undergoes activation and triggers holin-lysin-mediated bacterial lysis, leading to the production of infectious phage particles and increased extracellular recovery of bacterial vesicles carrying lipoteichoic acids. In a model of nutritional symbiosis, pp2-dependent lysis is required for LpNC8 to promote juvenile growth in undernourished Drosophila melanogaster. Disruption of prophage-mediated lysis impairs vesicle recovery and host growth promotion. Furthermore, we demonstrate that the copper cell region of the Drosophila midgut acts as a physiological trigger of prophage induction. Together, our findings link host intestinal physiology to prophage activity and bacterial lysis, revealing phage-driven lysis as a mechanism contributing to beneficial bacterial-host interactions during nutritional stress.

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Cite

BibTeX RIS

BibTeX

@article{zhu2026hosttriggered,
  title = {{Host-triggered prophage induction drives Lactiplantibacillus plantarum symbiosis through bacterial lysis}},
  author = {Chenxu Zhu and Sylvie Manuse and Quentin Perrier and Subhasree Venkatasubramaniyan and Houssam Akherraz and Norbert Lodej and Cathy I. Ramos and Meiling Zhang and Christophe Grangeasse and Agnieszka Razim and François Leulier and Renata C. Matos},
  journal = {Cell Reports},
  year = {2026},
  month = oct,
  doi = {10.1016/j.celrep.2026.118110},
  url = {https://hal.science/hal-05788076v1},
  note = {CC BY 4.0},
}

RIS

TY  - JOUR
TI  - Host-triggered prophage induction drives Lactiplantibacillus plantarum symbiosis through bacterial lysis
AU  - Chenxu Zhu
AU  - Sylvie Manuse
AU  - Quentin Perrier
AU  - Subhasree Venkatasubramaniyan
AU  - Houssam Akherraz
AU  - Norbert Lodej
AU  - Cathy I. Ramos
AU  - Meiling Zhang
AU  - Christophe Grangeasse
AU  - Agnieszka Razim
AU  - François Leulier
AU  - Renata C. Matos
PY  - 2026
DA  - 2026/10/01
JO  - Cell Reports
DO  - 10.1016/j.celrep.2026.118110
UR  - https://hal.science/hal-05788076v1
AB  - The release of bacterial bioactive molecules across the gut barrier is a crucial yet poorly understood step in host-microbe communication. Here, we identify a phage-driven mechanism associated with this process in the beneficial symbiont Lactiplantibacillus plantarum NC8 (LpNC8). We show that a stress-responsive prophage, pp2, undergoes activation and triggers holin-lysin-mediated bacterial lysis, leading to the production of infectious phage particles and increased extracellular recovery of bacterial vesicles carrying lipoteichoic acids. In a model of nutritional symbiosis, pp2-dependent lysis is required for LpNC8 to promote juvenile growth in undernourished Drosophila melanogaster. Disruption of prophage-mediated lysis impairs vesicle recovery and host growth promotion. Furthermore, we demonstrate that the copper cell region of the Drosophila midgut acts as a physiological trigger of prophage induction. Together, our findings link host intestinal physiology to prophage activity and bacterial lysis, revealing phage-driven lysis as a mechanism contributing to beneficial bacterial-host interactions during nutritional stress.
SN  - 2211-1247
SN  - 2211-1247
ER  -

Abstract

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