Casein Hydrolysate Peptides Attenuate Immunosenescence and Inflammaging in a D-Galactose-Induced Mouse Model of Aging.
- 1College of Food Engineering, East University of Heilongjiang, Harbin 150100, China.
- 2Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150036, China.
- 3Heilongjiang Feihe Dairy Co., Ltd., Harbin 150100, China.
Abstract
Aging populations worldwide face increasing burdens from physiological deterioration. Bioactive peptides derived from milk proteins have attracted interest for their biological activities, yet systematic evidence against aging remains limited. Here, we investigated casein hydrolysates in a D-galactose-induced murine aging model. Following eight weeks of oral administration at graded doses, we quantified systemic oxidative stress markers, inflammatory cytokine profiles, hepatic and cerebral histopathology, and multiple immune competence parameters. Our data demonstrate that casein peptides dose-dependently suppressed lipid peroxidation, elevated endogenous antioxidant enzyme activities, and rebalanced cytokine networks toward an anti-inflammatory state. Concurrently, peptide treatment attenuated tissue lesions in both liver and brain, while restoring thymus and spleen indices, normalizing T-cell subset distributions, and recovering serum immunoglobulin levels. These findings indicate that oral administration of the casein hydrolysate peptide preparation was associated with improvements in redox homeostasis, inflammatory status, tissue integrity, and immune function in D-galactose-induced aging mice. This work provides mechanistic insights into casein peptides as a multi-target protective agent against age-associated physiological decline, supporting further exploration of their therapeutic potential in aging biology.
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BibTeX
@article{cui2026casein,
title = {{Casein Hydrolysate Peptides Attenuate Immunosenescence and Inflammaging in a D-Galactose-Induced Mouse Model of Aging.}},
author = {Mengni Cui and Zheng Xu and Jianbo Cheng and Jikun Zhang and Caihong Hu and Yun Zhang},
journal = {Biology},
year = {2026},
month = oct,
doi = {10.3390/biology15191741},
url = {https://europepmc.org/article/MED/42883414},
note = {CC BY},
}
RIS
TY - JOUR
TI - Casein Hydrolysate Peptides Attenuate Immunosenescence and Inflammaging in a D-Galactose-Induced Mouse Model of Aging.
AU - Mengni Cui
AU - Zheng Xu
AU - Jianbo Cheng
AU - Jikun Zhang
AU - Caihong Hu
AU - Yun Zhang
PY - 2026
DA - 2026/10/01
JO - Biology
DO - 10.3390/biology15191741
UR - https://europepmc.org/article/MED/42883414
AB - Aging populations worldwide face increasing burdens from physiological deterioration. Bioactive peptides derived from milk proteins have attracted interest for their biological activities, yet systematic evidence against aging remains limited. Here, we investigated casein hydrolysates in a D-galactose-induced murine aging model. Following eight weeks of oral administration at graded doses, we quantified systemic oxidative stress markers, inflammatory cytokine profiles, hepatic and cerebral histopathology, and multiple immune competence parameters. Our data demonstrate that casein peptides dose-dependently suppressed lipid peroxidation, elevated endogenous antioxidant enzyme activities, and rebalanced cytokine networks toward an anti-inflammatory state. Concurrently, peptide treatment attenuated tissue lesions in both liver and brain, while restoring thymus and spleen indices, normalizing T-cell subset distributions, and recovering serum immunoglobulin levels. These findings indicate that oral administration of the casein hydrolysate peptide preparation was associated with improvements in redox homeostasis, inflammatory status, tissue integrity, and immune function in D-galactose-induced aging mice. This work provides mechanistic insights into casein peptides as a multi-target protective agent against age-associated physiological decline, supporting further exploration of their therapeutic potential in aging biology.
SN - 2079-7737
ER -