Effect of Green‐Synthesized Silver Nanoparticles From Nepeta pogonosperma and Astrodaucus persicus on the Reduction of Bap Gene Expression in Strong Biofilm‐Producing Acinetobacter baumannii Clinical Isolates
5 affiliations
- 1Immunogenetics Research Centre, Faculty of Medicine Mazandaran University of Medical Sciences Sari Iran
- 2Department of Medical Microbiology and Virology, Faculty of Medicine Mazandaran University of Medical Sciences Sari Iran
- 3Pharmaceutical Sciences Research Center, School of Pharmacy Mazandaran University of Medical Sciences Sari Iran
- 4Department of Biostatistics and Epidemiology, Faculty of Health Mazandaran University of Medical Sciences Sari Iran
- 5Molecular and Cell Biology Research Center, Hemoglobinopathy Institute, Faculty of Medicine Mazandaran University of Medical Sciences Sari Iran
Abstract
Given the critical role of the bap gene in biofilm formation of Acinetobacter baumannii, this study aimed to evaluate the efficacy of green‐synthesized silver nanoparticles (AgNPs) derived from Nepeta pogonosperma and Astrodaucus persicus (Boiss) in reducing the expression of this gene and inhibiting biofilm production in clinical isolates of A. baumannii. AgNPs were synthesized using the leaf extracts of the plants and characterized using standard techniques. The minimum inhibitory concentration of the AgNPs was determined by the microbroth dilution method. Biofilm production and the anti‐biofilm effects of the nanoparticles were assessed using a microtiter plate assay. Quantitative real‐time PCR was employed to evaluate bap gene expression levels before and after treatment with the AgNPs. The AgNPs significantly inhibited biofilm formation in A. baumannii. Furthermore, a marked reduction in bap gene expression was observed following treatment. Moreover, 90% and 10% of the isolates showed up to 60% and 61%–80% of biofilm disruption after treatment with A. persicus AgNP, respectively. In addition, 80%, 15%, and 5% of the isolates showed up to 60%, 61%–80%, and more than 80% of biofilm disruption after treatment with N. pogonosperma AgNP, respectively. Green synthesis of silver nanoparticles represent a promising strategy to counteract antimicrobial resistance. The AgNPs investigated in this study demonstrated significant potential in inhibiting biofilm formation and reducing bap gene expression in strong biofilm‐producing clinical isolates of A. baumannii. Further research is warranted to elucidate the underlying mechanisms and optimize the clinical application of these nanoparticles.
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BibTeX
@article{kakavan2026effect,
title = {{Effect of Green‐Synthesized Silver Nanoparticles From Nepeta pogonosperma and Astrodaucus persicus on the Reduction of Bap Gene Expression in Strong Biofilm‐Producing Acinetobacter baumannii Clinical Isolates}},
author = {Maedeh Kakavan and Mehrdad Gholami and Mohammad Ahanjan and Mohammad Ali Ebrahimzadeh and Abolfazl Hossein Nataj and Tahoora Mousavi and Hamid Reza Goli},
journal = {MicrobiologyOpen},
year = {2026},
month = oct,
doi = {10.1002/mbo3.70391},
url = {https://doi.org/10.1002/mbo3.70391},
note = {CC BY},
}
RIS
TY - JOUR
TI - Effect of Green‐Synthesized Silver Nanoparticles From Nepeta pogonosperma and Astrodaucus persicus on the Reduction of Bap Gene Expression in Strong Biofilm‐Producing Acinetobacter baumannii Clinical Isolates
AU - Maedeh Kakavan
AU - Mehrdad Gholami
AU - Mohammad Ahanjan
AU - Mohammad Ali Ebrahimzadeh
AU - Abolfazl Hossein Nataj
AU - Tahoora Mousavi
AU - Hamid Reza Goli
PY - 2026
DA - 2026/10/27
JO - MicrobiologyOpen
DO - 10.1002/mbo3.70391
UR - https://doi.org/10.1002/mbo3.70391
AB - Given the critical role of the bap gene in biofilm formation of Acinetobacter baumannii, this study aimed to evaluate the efficacy of green‐synthesized silver nanoparticles (AgNPs) derived from Nepeta pogonosperma and Astrodaucus persicus (Boiss) in reducing the expression of this gene and inhibiting biofilm production in clinical isolates of A. baumannii. AgNPs were synthesized using the leaf extracts of the plants and characterized using standard techniques. The minimum inhibitory concentration of the AgNPs was determined by the microbroth dilution method. Biofilm production and the anti‐biofilm effects of the nanoparticles were assessed using a microtiter plate assay. Quantitative real‐time PCR was employed to evaluate bap gene expression levels before and after treatment with the AgNPs. The AgNPs significantly inhibited biofilm formation in A. baumannii. Furthermore, a marked reduction in bap gene expression was observed following treatment. Moreover, 90% and 10% of the isolates showed up to 60% and 61%–80% of biofilm disruption after treatment with A. persicus AgNP, respectively. In addition, 80%, 15%, and 5% of the isolates showed up to 60%, 61%–80%, and more than 80% of biofilm disruption after treatment with N. pogonosperma AgNP, respectively. Green synthesis of silver nanoparticles represent a promising strategy to counteract antimicrobial resistance. The AgNPs investigated in this study demonstrated significant potential in inhibiting biofilm formation and reducing bap gene expression in strong biofilm‐producing clinical isolates of A. baumannii. Further research is warranted to elucidate the underlying mechanisms and optimize the clinical application of these nanoparticles.
SN - 2045-8827
ER -