Skip to content
DraconiticResearch
Search Sources API
201,818 studies31 sourcesUpdated 49 min ago
Peer-reviewedCC BY-NC-NDBMC cancer

Association of regulatory B, T cells and cytokine production with disease stage in non-small cell lung cancer patients: prospective case control study.

  • Helal F Hetta1
  • Asmaa M Zahran2
  • Emad Eldin Nabil3
  • Azza A Elkady4
  • Asmaa M Goda5
  • Tamer M Soliman6
  • Abeer Elkady7
  • Hend M Esmaeel8,9
  • Abdelazeem M Algammal10
  • Nahed A Mohamed11
  • Hala M ElBadre11
  • Hani Alrefai12
2 more authors
  • Ashraf Elyamany13
  • Ola A Alkady14
14 affiliations
  1. 1Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, 71526, Egypt.
  2. 2Clinical Pathology Department, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
  3. 3Department of Clinical Oncology, Faculty of medicine, Sohag University, Sohag, Egypt.
  4. 4Sohag University medical administration, Sohag, Egypt.
  5. 5Department of Medical Microbiology and Immunology, Faculty of Medicine, Sohag University, Sohag, Egypt.
  6. 6Department of Clinical Pathology, Faculty of Medicine, Sohag University, Sohag, Egypt.
  7. 7Department of Clinical and Chemical Pathology, Faculty of Medicine, Qena University, Qena, Egypt.
  8. 8Department of Chest Diseases and Tuberculosis, Faculty of Medicine, Sohag University, Sohag, Egypt. [email protected].
  9. 9Department of Chest Diseases and Tuberculosis, Faulty of medicine, Sohag University, Sohag, Egypt. [email protected].
  10. 10Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
  11. 11Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Assiut, Egypt.
  12. 12Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura City, 35516, Egypt.
  13. 13Department of Medical oncology south Egypt cancer institute Assiut University, Assiut, Egypt.
  14. 14Department of Chest Diseases and Tuberculosis, Faculty of Medicine, Sohag University, Sohag, Egypt.
Read full textPDFDOICite

Abstract

Background and aim

The role of regulatory B cells (Bregs) cells and regulatory T cells (Tregs) has been little investigated in non-small cell lung cancer (NSCLC). Therefore, we investigated the frequency of circulating Bregs, Tregs cells, and the serum cytokine profile in NSCLC and their association with disease progression.Materials and methods

In this prospective case-control study, peripheral blood from 40 patients with NSCLC and 40 healthy controls was examined, the frequency of various lymphocyte subsets including Bregs and Tregs was evaluated using flow cytometry. Serum cytokine profile including IL1β, IL6, IL-17, TNF-α, IL10, IL35 and TGF-β1 was measured using Luminex assay.Results

An increased proportion of Bregs, Tregs, CD8 + T cells, and decreased CD4+/CD8 + ratio was observed in NSCLC patients compared to healthy controls. NSCLC patients with metastasis had a higher frequency of Bregs and Tregs than patients without metastasis. The frequency of Bregs and Tregs was related to the stage and histopathological status. NSCLC patients had significantly higher levels of serum cytokine profile when compared to healthy controls along with a significant association with lymph node metastasis and different TNM stages.Conclusions

Peripheral Bregs, Tregs, and serum cytokine profiles, including proinflammatory and anti-inflammatory cytokines were increased among NSCLC patients and may be associated with disease progression and metastasis.

Related

  1. Genome-wide association study identifies candidate loci and a functional regulatory variant associated with egg-production traits in Wenchang chickens
    Lingzhi Fan, Xing Hong, Mingze Li +4
    Poultry sciencePeer-reviewedCC BY-NC-NDFull textGenetics & genomicsEurope PMCPMC
  2. Effects of rhinovirus species on viral replication and cytokine production
    K. Nakagome, Y. A. Bochkov, S. Ashraf +4
    Journal of Allergy and Clinical ImmunologyPeer-reviewedLicence not stated117 citedMicrobiologyHAL
  3. FLT3LG induction by γ-chain cytokine promotes regulatory-like CD4+ T cell features in breast cancer
    Yeo-Jin Im, Hyeon-Ji Hwang, Chae-Yi Kim +1
    Cancer Immunology, Immunotherapy : CIIPeer-reviewedCC BY-NC-ND 4.0Full textImmunologyPMC
  4. Effect of bacteriophages on cytokine production by human peripheral blood mononuclear cells and their subpopulations
    Hubert Kasprzak, Monika Kniotek, Katarzyna Zielniok +3
    Frontiers in ImmunologyPeer-reviewedCC BY 4.0Full textEcologyCrossrefPMC
  5. Joint association of cytokine gene variants and circulating inflammatory markers with increased breast cancer risk
    Shuya Zheng, Fuqiang Cui, Jun Qin +1
    Scientific ReportsPeer-reviewedCC BY 4.0Full textGenetics & genomicsCrossref

Cite

BibTeXRIS
BibTeX
@article{hetta2026association,
  title = {{Association of regulatory B, T cells and cytokine production with disease stage in non-small cell lung cancer patients: prospective case control study.}},
  author = {Helal F Hetta and Asmaa M Zahran and Emad Eldin Nabil and Azza A Elkady and Asmaa M Goda and Tamer M Soliman and Abeer Elkady and Hend M Esmaeel and Abdelazeem M Algammal and Nahed A Mohamed and Hala M ElBadre and Hani Alrefai and Ashraf Elyamany and Ola A Alkady},
  journal = {BMC cancer},
  year = {2026},
  month = oct,
  doi = {10.1186/s12885-026-16464-7},
  url = {https://europepmc.org/article/MED/42875749},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Association of regulatory B, T cells and cytokine production with disease stage in non-small cell lung cancer patients: prospective case control study.
AU  - Helal F Hetta
AU  - Asmaa M Zahran
AU  - Emad Eldin Nabil
AU  - Azza A Elkady
AU  - Asmaa M Goda
AU  - Tamer M Soliman
AU  - Abeer Elkady
AU  - Hend M Esmaeel
AU  - Abdelazeem M Algammal
AU  - Nahed A Mohamed
AU  - Hala M ElBadre
AU  - Hani Alrefai
AU  - Ashraf Elyamany
AU  - Ola A Alkady
PY  - 2026
DA  - 2026/10/14
JO  - BMC cancer
DO  - 10.1186/s12885-026-16464-7
UR  - https://europepmc.org/article/MED/42875749
AB  - Background and aim  The role of regulatory B cells (Bregs) cells and regulatory T cells (Tregs) has been little investigated in non-small cell lung cancer (NSCLC). Therefore, we investigated the frequency of circulating Bregs, Tregs cells, and the serum cytokine profile in NSCLC and their association with disease progression.Materials and methods  In this prospective case-control study, peripheral blood from 40 patients with NSCLC and 40 healthy controls was examined, the frequency of various lymphocyte subsets including Bregs and Tregs was evaluated using flow cytometry. Serum cytokine profile including IL1β, IL6, IL-17, TNF-α, IL10, IL35 and TGF-β1 was measured using Luminex assay.Results  An increased proportion of Bregs, Tregs, CD8 + T cells, and decreased CD4+/CD8 + ratio was observed in NSCLC patients compared to healthy controls. NSCLC patients with metastasis had a higher frequency of Bregs and Tregs than patients without metastasis. The frequency of Bregs and Tregs was related to the stage and histopathological status. NSCLC patients had significantly higher levels of serum cytokine profile when compared to healthy controls along with a significant association with lymph node metastasis and different TNM stages.Conclusions  Peripheral Bregs, Tregs, and serum cytokine profiles, including proinflammatory and anti-inflammatory cytokines were increased among NSCLC patients and may be associated with disease progression and metastasis.
SN  - 1471-2407
ER  -