Emergence of Bacterial Leaf Blight of Rice in Madagascar: A Recent Introduction from Asia
Abstract
Xanthomonas oryzae pv. oryzae ( Xoo) causes bacterial leaf blight (BLB), a major rice disease causing up to 70% yield loss in Asia and West Africa. First described in Japan in 1884 and later reported in West Africa in the 1970s, BLB recently emerged in East Africa, with an epidemic reported in Tanzania in 2019. Remarkably, the disease was detected for the first time in Madagascar the same year, representing a serious threat to food security. To investigate the origin of BLB in Madagascar, we isolated 73 Xoo strains from symptomatic rice leaves collected between 2019 and 2023. Multilocus variable-number of tandem-repeats analysis genotyping revealed 19 haplotypes forming a single clonal complex, indicating low diversity and a likely recent introduction. To come up with disease control strategies, IRBB-based race-typing was achieved and identified four resistance genes ( Xa8, xa13, Xa21, and Xa23) that confer resistance to all Malagasy strains tested, whereas the 19 Malagasy varieties assessed were susceptible. The analysis of SWEET knockout lines confirmed that Malagasy strains rely on the susceptibility gene OsSWEET11 for full virulence. Whole-genome sequencing and transcription activator-like effector repertoire analyses of two strains allowed for the identification of a PthXo1 ortholog predicted to induce OsSWEET11. Single-nucleotide polymorphism-based phylogenetic analyses clustered Malagasy strains within Asian lineages, most closely related to strains originated from India. Malagasy strains did not cluster with recently reported Tanzanian Xoo, suggesting independent introductions. Overall, our study demonstrates that BLB in Madagascar results from a recent and single introduction from Asia and identifies effective resistance genes for deployment.
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BibTeX
@article{rabekijana2026emergence,
title = {{Emergence of Bacterial Leaf Blight of Rice in Madagascar: A Recent Introduction from Asia}},
author = {Ravonantenaina Rabekijana and Harinjaka Raveloson and Alexis Dereeper and Coline Sciallano and Gabriel Boulard and Florence Auguy and Natolotra Rakotonanahary and Herizo Rakotonary and Emilie Thomas and Laurent Brottier and Sebastien Cunnac and Boris Szurek and Mathilde Hutin},
journal = {Phytopathology},
year = {2026},
month = jun,
doi = {10.1094/phyto-10-25-0336-sc},
url = {https://institut-agro-montpellier.hal.science/hal-05839342v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Emergence of Bacterial Leaf Blight of Rice in Madagascar: A Recent Introduction from Asia
AU - Ravonantenaina Rabekijana
AU - Harinjaka Raveloson
AU - Alexis Dereeper
AU - Coline Sciallano
AU - Gabriel Boulard
AU - Florence Auguy
AU - Natolotra Rakotonanahary
AU - Herizo Rakotonary
AU - Emilie Thomas
AU - Laurent Brottier
AU - Sebastien Cunnac
AU - Boris Szurek
AU - Mathilde Hutin
PY - 2026
DA - 2026/06/01
JO - Phytopathology
DO - 10.1094/phyto-10-25-0336-sc
UR - https://institut-agro-montpellier.hal.science/hal-05839342v1
AB - Xanthomonas oryzae pv. oryzae ( Xoo) causes bacterial leaf blight (BLB), a major rice disease causing up to 70% yield loss in Asia and West Africa. First described in Japan in 1884 and later reported in West Africa in the 1970s, BLB recently emerged in East Africa, with an epidemic reported in Tanzania in 2019. Remarkably, the disease was detected for the first time in Madagascar the same year, representing a serious threat to food security. To investigate the origin of BLB in Madagascar, we isolated 73 Xoo strains from symptomatic rice leaves collected between 2019 and 2023. Multilocus variable-number of tandem-repeats analysis genotyping revealed 19 haplotypes forming a single clonal complex, indicating low diversity and a likely recent introduction. To come up with disease control strategies, IRBB-based race-typing was achieved and identified four resistance genes ( Xa8, xa13, Xa21, and Xa23) that confer resistance to all Malagasy strains tested, whereas the 19 Malagasy varieties assessed were susceptible. The analysis of SWEET knockout lines confirmed that Malagasy strains rely on the susceptibility gene OsSWEET11 for full virulence. Whole-genome sequencing and transcription activator-like effector repertoire analyses of two strains allowed for the identification of a PthXo1 ortholog predicted to induce OsSWEET11. Single-nucleotide polymorphism-based phylogenetic analyses clustered Malagasy strains within Asian lineages, most closely related to strains originated from India. Malagasy strains did not cluster with recently reported Tanzanian Xoo, suggesting independent introductions. Overall, our study demonstrates that BLB in Madagascar results from a recent and single introduction from Asia and identifies effective resistance genes for deployment.
SN - 0031-949X
SN - 0031-949X
ER -