Carriage and susceptibility of Enterobacteria isolated from the local chickens at markets and peri-urban areas of Bobo-Dioulasso, Burkina Faso
4 affiliations
- 1Laboratoire de Recherche et d’Enseignement en Santé et Biotechnologies Animales, Université Nazi BONI (UNB)
- 2Laboratoire de Recherche en Bactériologie, INSP/Centre MURAZ
- 3Laboratoire de Médicine et Pharmacopée Traditionnelle, Institut de Recherche en Sciences de la Santé, Direction Régionale de l’Ouest
- 4Department of Clinical Nutrition, Faculty of Applied Medical Sciences, Umm Al-Qura University
Abstract
Background Salmonella and Escherichia coli are among the main causes of foodborne illness and negatively impact poultry industry. The aim of this study is to determine the carriage rate and sensitivity of Enterobacteria isolated from the cloacae of chickens. Samples were taken only from healthy local chickens of markets (55 samples) and outlying areas (45 samples) using the swabbing technique. The swabs were pre-enriched and inoculated onto Hektoen agar at 37 °C for 24 h. Bacteria and resistance genes were identified through molecular biology. Antimicrobial susceptibility was tested by Kirby Bauer method. Results The overall prevalence was 65% for Escherichia coli and 27% for Salmonella sp. after biochemical tests and these carrying rates of these strains were 10% for Salmonella and 53% for Escherichia coli after molecular confirmation tests. E. coli isolates (n = 53), 91.43% (32/35) originated from farms, while 75% (21/28) were collected from markets. The detection of the 16S rRNA gene was higher in market samples (25%) compared to farm samples (8.57%). Antibiotic susceptibility testing showed extremely high resistance rates to amoxicillin (> 93%) and tetracycline (> 80%) in both bacterial species. In contrast, high susceptibility rates were recorded to ceftriaxone (> 90%) and norfloxacin (> 89%). Moderate resistance levels were observed to cotrimoxazole and amoxicillin-clavulanic acid Augmentin (> 41%). Comparative statistical analysis between bacterial species showed no statistically significant difference for most antibiotics (p > 0.05). Among Escherichia coli isolates, 58.49% (31/53) exhibited a penicillinase phenotype, compared with 60% (6/10) of Salmonella spp. and ESBL phenotypes were detected in 35.80% (19/53) of E. coli isolates and 40% (4/10) of Salmonella spp. isolates. No statistically significant difference was observed between the species. The bla TEM gene was detected in 41.5% (22/53) of E. coli isolates and in 60% (6/10) of Salmonella spp. isolates. Similarly, the bla CTX-M-9 gene was identified in 35.58% (19/53) of E. coli isolates and 30% (3/10) of Salmonella spp. isolates although this difference was not statistically significant (p > 0.05). Conclusion This study highlighted the presence of strains Enterobacteria resistant to many antimicrobials of therapeutic importance in healthy chickens. For the well-being of consumers, it is necessary to strengthen surveillance and prevention systems.
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BibTeX
@article{zon2026carriage,
title = {{Carriage and susceptibility of Enterobacteria isolated from the local chickens at markets and peri-urban areas of Bobo-Dioulasso, Burkina Faso}},
author = {Drissa Zon and Victorien Yaméogo and Roland Nâg-Tiero Meda and Soufiane Sanou and Benjamin Kouliga Koama and Yerbanga R. Serge and Sami Eric Kam and Eliasse Zongo and Kobo Gnada and Georges Anicet Ouédraogo and Mohamed Fawzy Ramadan},
journal = {BMC Microbiology},
year = {2026},
month = jul,
doi = {10.1186/s12866-026-05394-w},
url = {https://doi.org/10.1186/s12866-026-05394-w},
note = {CC BY},
}
RIS
TY - JOUR
TI - Carriage and susceptibility of Enterobacteria isolated from the local chickens at markets and peri-urban areas of Bobo-Dioulasso, Burkina Faso
AU - Drissa Zon
AU - Victorien Yaméogo
AU - Roland Nâg-Tiero Meda
AU - Soufiane Sanou
AU - Benjamin Kouliga Koama
AU - Yerbanga R. Serge
AU - Sami Eric Kam
AU - Eliasse Zongo
AU - Kobo Gnada
AU - Georges Anicet Ouédraogo
AU - Mohamed Fawzy Ramadan
PY - 2026
DA - 2026/07/01
JO - BMC Microbiology
DO - 10.1186/s12866-026-05394-w
UR - https://doi.org/10.1186/s12866-026-05394-w
AB - Background Salmonella and Escherichia coli are among the main causes of foodborne illness and negatively impact poultry industry. The aim of this study is to determine the carriage rate and sensitivity of Enterobacteria isolated from the cloacae of chickens. Samples were taken only from healthy local chickens of markets (55 samples) and outlying areas (45 samples) using the swabbing technique. The swabs were pre-enriched and inoculated onto Hektoen agar at 37 °C for 24 h. Bacteria and resistance genes were identified through molecular biology. Antimicrobial susceptibility was tested by Kirby Bauer method. Results The overall prevalence was 65% for Escherichia coli and 27% for Salmonella sp. after biochemical tests and these carrying rates of these strains were 10% for Salmonella and 53% for Escherichia coli after molecular confirmation tests. E. coli isolates (n = 53), 91.43% (32/35) originated from farms, while 75% (21/28) were collected from markets. The detection of the 16S rRNA gene was higher in market samples (25%) compared to farm samples (8.57%). Antibiotic susceptibility testing showed extremely high resistance rates to amoxicillin (> 93%) and tetracycline (> 80%) in both bacterial species. In contrast, high susceptibility rates were recorded to ceftriaxone (> 90%) and norfloxacin (> 89%). Moderate resistance levels were observed to cotrimoxazole and amoxicillin-clavulanic acid Augmentin (> 41%). Comparative statistical analysis between bacterial species showed no statistically significant difference for most antibiotics (p > 0.05). Among Escherichia coli isolates, 58.49% (31/53) exhibited a penicillinase phenotype, compared with 60% (6/10) of Salmonella spp. and ESBL phenotypes were detected in 35.80% (19/53) of E. coli isolates and 40% (4/10) of Salmonella spp. isolates. No statistically significant difference was observed between the species. The bla TEM gene was detected in 41.5% (22/53) of E. coli isolates and in 60% (6/10) of Salmonella spp. isolates. Similarly, the bla CTX-M-9 gene was identified in 35.58% (19/53) of E. coli isolates and 30% (3/10) of Salmonella spp. isolates although this difference was not statistically significant (p > 0.05). Conclusion This study highlighted the presence of strains Enterobacteria resistant to many antimicrobials of therapeutic importance in healthy chickens. For the well-being of consumers, it is necessary to strengthen surveillance and prevention systems.
SN - 1471-2180
ER -