Type VII secretion system promotes Streptococcus agalactiae virulence through magnesium acquisition and capsule maintenance
Abstract
Streptococcus agalactiae, also known as group B Streptococcus (GBS), is a major pathogen causing substantial economic losses in global tilapia aquaculture. The type VII secretion system (T7SS), present in Actinobacteria and Firmicutes, secretes effector proteins implicated in bacterial virulence, yet its functional mechanisms remain poorly defined. Here, we constructed an essC deletion mutant (∆essC) in S. agalactiae strain HN016 to investigate the role of T7SS in virulence. The ∆essC exhibited impaired growth and declined intracellular magnesium ion concentration in THB or magnesium-limited chemically defined medium (1 mM Mg2+-CDM); these defects were complemented by addition of wild-type culture supernatant. Transcriptomic and quantitative reverse transcription polymerase chain reaction (qRT–PCR) analyses revealed altered sugar metabolism and significant downregulation of capsule biosynthesis genes (cpsA, cpsB, and cpsD) in ∆essC. Consistent with this, the mutant produced markedly less capsular polysaccharide and displayed impaired capsule integrity. Furthermore, cellular assays confirmed that compared with HN016, the ∆essC mutant exhibited significantly reduced adhesion capacity, immune evasion ability, and cytotoxicity. More importantly, the ∆essC mutant showed attenuated virulence in vivo, with reduced bacterial loads in host tissues and diminished ability to cross the blood–brain barrier (BBB). Our findings provide the first evidence that the T7SS influences magnesium homeostasis and is essential for maintaining capsule integrity, both of which contribute critically to pathogenicity. This study identifies T7SS as a potential target for novel therapeutic strategies against streptococcal disease in aquaculture.
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BibTeX
@article{li2026type,
title = {{Type VII secretion system promotes Streptococcus agalactiae virulence through magnesium acquisition and capsule maintenance}},
author = {Fengyang Li and Chen Xu and Weiyi Ma and Yong Zhou and Yang Zhou and Yong-An Zhang and Hui Zeng},
journal = {Veterinary Research},
year = {2026},
month = aug,
doi = {10.1186/s13567-026-01816-9},
url = {https://hal.science/hal-05731886v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Type VII secretion system promotes Streptococcus agalactiae virulence through magnesium acquisition and capsule maintenance
AU - Fengyang Li
AU - Chen Xu
AU - Weiyi Ma
AU - Yong Zhou
AU - Yang Zhou
AU - Yong-An Zhang
AU - Hui Zeng
PY - 2026
DA - 2026/08/31
JO - Veterinary Research
DO - 10.1186/s13567-026-01816-9
UR - https://hal.science/hal-05731886v1
AB - Streptococcus agalactiae, also known as group B Streptococcus (GBS), is a major pathogen causing substantial economic losses in global tilapia aquaculture. The type VII secretion system (T7SS), present in Actinobacteria and Firmicutes, secretes effector proteins implicated in bacterial virulence, yet its functional mechanisms remain poorly defined. Here, we constructed an essC deletion mutant (∆essC) in S. agalactiae strain HN016 to investigate the role of T7SS in virulence. The ∆essC exhibited impaired growth and declined intracellular magnesium ion concentration in THB or magnesium-limited chemically defined medium (1 mM Mg2+-CDM); these defects were complemented by addition of wild-type culture supernatant. Transcriptomic and quantitative reverse transcription polymerase chain reaction (qRT–PCR) analyses revealed altered sugar metabolism and significant downregulation of capsule biosynthesis genes (cpsA, cpsB, and cpsD) in ∆essC. Consistent with this, the mutant produced markedly less capsular polysaccharide and displayed impaired capsule integrity. Furthermore, cellular assays confirmed that compared with HN016, the ∆essC mutant exhibited significantly reduced adhesion capacity, immune evasion ability, and cytotoxicity. More importantly, the ∆essC mutant showed attenuated virulence in vivo, with reduced bacterial loads in host tissues and diminished ability to cross the blood–brain barrier (BBB). Our findings provide the first evidence that the T7SS influences magnesium homeostasis and is essential for maintaining capsule integrity, both of which contribute critically to pathogenicity. This study identifies T7SS as a potential target for novel therapeutic strategies against streptococcal disease in aquaculture.
SN - 0928-4249
SN - 1297-9716
ER -