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Peer-reviewedCC BY 4.0Frontiers in Immunology

Oligopeptide-binding protein A is a potential target for combating Salmonella infection

  • Ken Yoshii1,2
  • Koichiro Suzuki1,3,4
  • Jun Adachi5
  • Zhenyu Piao3
  • Eri Node1
  • Zilai Liu1,6
  • Takahiro Nagatake1,7
  • Koji Hosomi1,8
  • Jun Kunisawa1,6,9,10,11,12,13,14,15
15 affiliations
  1. 1Laboratory of Vaccine Materials and Laboratory of Gut Environmental System, Microbial Research Center for Health and Medicine, National Institutes of Biomedical Innovation, Health and Nutrition (NIBN)
  2. 2Biomanufacturing Process Research Center, National Institute of Advanced and Industrial Science and Technology (AIST)
  3. 3The Research Foundation for Microbial Diseases of The University of Osaka
  4. 4BIKEN-RIMD NGS Laboratory, Research Institute for Microbial Diseases, The University of Osaka
  5. 5Laboratory of Proteomics for Drug Discovery, National Institutes of Biomedical Innovation, Health and Nutrition (NIBN)
  6. 6Graduate School of Pharmaceutical Sciences, The University of Osaka
  7. 7Laboratory of Functional Anatomy, Department of Life Sciences, School of Agriculture, Meiji University
  8. 8Graduate School of Veterinary Science, Osaka Metropolitan University
  9. 9Graduate School of Medicine, The University of Osaka
  10. 10Graduate School of Dentistry, The University of Osaka
  11. 11Graduate School of Science, The University of Osaka
  12. 12International Vaccine Design Center, The Institute of Medical Science, The University of Tokyo
  13. 13Department of Microbiology and Immunology, Kobe University Graduate School of Medicine
  14. 14Graduate School of Biomedical and Health Sciences, Hiroshima University
  15. 15Research Organization for Nano and Life Innovation, Waseda University
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Abstract

Introduction: Salmonella infection remains a pervasive diarrheal illness, particularly affecting populations in developing countries. The lack of an effective vaccine against non-typhoidal Salmonella is attributable to antigenic diversity among serotypes, incomplete understanding of protective immune mechanisms, and the difficulty in identifying conserved antigens capable of eliciting broad cross-protective immunity.

Methods: To identify conserved Salmonella antigens, we established a Salmonella -specific monoclonal antibody library using heat-killed bacteria. Candidate antigens recognized by monoclonal antibodies were characterized, and their functional relevance was evaluated through analyses of bacterial growth, respiratory activity, and protective effects in a murine Salmonella infection model.

Results: We identified L2-1H11, a monoclonal antibody that recognizes OppA, a conserved antigen present across multiple Salmonella serotypes. L2-1H11 was associated with suppression of bacterial growth and respiratory activity and attenuated enteritis pathology in Salmonella -infected mice.

Conclusion: These findings identify OppA as a conserved Salmonella antigen and suggest that it may represent a promising target for the development of subcutaneous and oral vaccines against Salmonella .

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Cite

BibTeXRIS
BibTeX
@article{yoshii2026oligopeptidebinding,
  title = {{Oligopeptide-binding protein A is a potential target for combating Salmonella infection}},
  author = {Ken Yoshii and Koichiro Suzuki and Jun Adachi and Zhenyu Piao and Eri Node and Zilai Liu and Takahiro Nagatake and Koji Hosomi and Jun Kunisawa},
  journal = {Frontiers in Immunology},
  year = {2026},
  month = oct,
  doi = {10.3389/fimmu.2026.1943609},
  url = {https://www.frontiersin.org/articles/10.3389/fimmu.2026.1943609/full},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Oligopeptide-binding protein A is a potential target for combating Salmonella infection
AU  - Ken Yoshii
AU  - Koichiro Suzuki
AU  - Jun Adachi
AU  - Zhenyu Piao
AU  - Eri Node
AU  - Zilai Liu
AU  - Takahiro Nagatake
AU  - Koji Hosomi
AU  - Jun Kunisawa
PY  - 2026
DA  - 2026/10/09
JO  - Frontiers in Immunology
DO  - 10.3389/fimmu.2026.1943609
UR  - https://www.frontiersin.org/articles/10.3389/fimmu.2026.1943609/full
AB  - Introduction: Salmonella infection remains a pervasive diarrheal illness, particularly affecting populations in developing countries. The lack of an effective vaccine against non-typhoidal Salmonella is attributable to antigenic diversity among serotypes, incomplete understanding of protective immune mechanisms, and the difficulty in identifying conserved antigens capable of eliciting broad cross-protective immunity.  Methods: To identify conserved Salmonella antigens, we established a Salmonella -specific monoclonal antibody library using heat-killed bacteria. Candidate antigens recognized by monoclonal antibodies were characterized, and their functional relevance was evaluated through analyses of bacterial growth, respiratory activity, and protective effects in a murine Salmonella infection model.  Results: We identified L2-1H11, a monoclonal antibody that recognizes OppA, a conserved antigen present across multiple Salmonella serotypes. L2-1H11 was associated with suppression of bacterial growth and respiratory activity and attenuated enteritis pathology in Salmonella -infected mice.  Conclusion: These findings identify OppA as a conserved Salmonella antigen and suggest that it may represent a promising target for the development of subcutaneous and oral vaccines against Salmonella .
SN  - 1664-3224
ER  -